解鏈酶 的英文怎麼說
中文拼音 [jiěliàn]
解鏈酶
英文
desmolase-
Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明, arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝: ( 1 ) arg可能影響胞外蛋白酶的活性,進而促進含氮大分子物質的分解代謝,補充發酵過程中的氮素來源。Like light meromyosin, tropomyosin contains two peptide chains in a coiled coil conformation.
象輕酶解肌球蛋白一樣,原肌球蛋白含有兩個肽鏈,其構型為盤旋螺旋。Elevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus
這樣,可能使得萎縮比目魚肌細胞內鈣離子水平升高,細胞內鈣離子靜息濃度的增加一方面激活calpain ,增加收縮蛋白的降解,使肌肉萎縮;草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶,增加快型mhc基因的表達,使骨骼肌肌球蛋白重鏈( mhc )發生由慢型向快型的轉化。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Helicase any of a group of enzymes that catalyze the unwinding of double - stranded nucleic acids such as dna, using energy from atp
解螺旋酶(蝸牛酶) :一組可以催化象dna這樣雙鏈核酸解螺旋的酶,由atp提供能量。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體形成和再生的主要因素,確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶菌酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生菌落;後者採用二級菌絲培養,用1mg ml的溶菌酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生菌。A. enzymes transform complex, recalcitrant aromatic and aliphatic hydrocarbons that are not easily biodegradable to simple fatty acids, thereby accelerating biological cleanup
答:酶能轉化復雜的,難降解的芳烴和直鏈烴類化合物,並不是輕易地將它們生物降解為為簡單的脂肪酸,而是加速生物清除的過程。However, the ubiquitylated substrate protein of these ubcs remains unknown, and the nature of the dna to which the repair factors bind is still unclear
這三種酶協同作用把泛素分子鏈作為標簽連接在目的蛋白上使之被26s蛋白酶體識別而降解。A defective protein, which binds polyubiquitin chain ( so - called " degrading label " ) through its - amino group of lys residue, is degraded in the proteasome
對于需要清除的蛋白質,通過其賴氨酸殘基側鏈-氨基連接多聚泛素鏈(降解標簽) ,繼而在蛋白酶體中被降解。Protease ( proteinase ) an enzyme that catalyzes the hydrolysis of peptide bonds in proteins to produce peptide chains and amino acids
蛋白酶:指催化蛋白質中肽鍵水解為多肽鏈和氨基酸的酶。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白( f )基因裂解位點的核苷酸序列與其毒力的相關規律,分別設計合成了四條寡核苷酸引物,建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應( rt - pcr )技術。Tests were carried out to examine the substrate characteristics of the esterase and the results showed that the esterase could hydrolyze not only lactic acetate, triacetin, and so on, with low molecular weight, but also the esters with high molecular weight
酯酶的底物特異性試驗的結果看出,該酯酶不但能分解乳酸乙酯、三乙酸甘油酯等低分子量的酯,也能水解多碳鏈酯,所以含有脂肪酶的性質。分享友人