Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設
計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
Your monthly salary amounts to $1500.
你的月薪總
計一千五百美元。
Designing an incentive and operative salary system which basic salary as it " s center is most important to attract core stuffs and bestir a great of employees
設
計一個以基本薪為中心的、具有激勵性和可操作性的薪酬體系對企業吸引保留核心員工以及激勵廣大員工極為重要。
The architect was given / presented with a blank cheque to design a new city centre
設
計師被授予全權設
計一個新的城市中心區
The capstone project in this course is the final design project in which each student designs a sailing yacht, complete in all major respects
本課程的最終
計劃是期末設
計專題，在這個專題內，每個學生要設
計一艘主要功能完備的遊艇。
So to economize capital and raise productivity, it is essential to design an advanced transport equipment in the carbamide be1t conveyor line transportation reforming project
因此，為節約資金、提高效率，設
計一套先進的運輸設備是必不可少的。
In the following months, he demanded a design for an armed caterpillar tractor, capable of crossing trenches and crashing obstacles
在隨后的月份里，他要求設
計一個裝備武器的能翻過壕溝和碾碎障礙物的履帶車。
In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設
計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
In lots of implementation fields of ai, expert system is a classis intelligent implement system, whose goal is researching how to design a computer program bases on knowledge, to simulate the ability that human expert solutes special problems
在眾多的人工智慧應用領域中，專家系統是近30年來發展起來的
一種極富代表性的智能應用系統，旨在研究如何設
計一種基於知識的
計算機程序系統來模仿人類專家求解專門問題的能力。
A trained clinician designs a treatment protocol based on the needs and particular behavioural problem of an individual patient.
一個經過訓練的醫生須根據具體病人的特殊行為問題的需要，來設
計一個治療方案。
Add up the figures in this column.
把這
一欄的數字合
計一下。
The coupons are valid for one year from the date of issue
有效期為發出日
計一年。
- whoa ! sheesh, man. - little ding. little ding
-看著點，伙
計-
一點點來，
一點點來
Whoa ! sheesh, man. - little ding. little ding
看著點，伙
計-
一點點來，
一點點來
For your information, we are planning a range of classical english dinner services which should do well in the north american market
本公司現正設
計一系列款式古典的英國餐具，適合北美市場需求.如感興趣，亦請賜知
In this paper, we design a kind of multi - discursion model based on episodic memory, construct the trading agent living in the flow of trade data stream in high - dimension capital market to research the market activeness inside
摘要設
計一種基於場景記憶的多層推理模型，構造生活在高維資本市場內部交易數據流中的感知交易主體，從市場的內部研究市場的交易行為。
This design plan will apply the above theory to design a duplex apartment, and it will focus on the planning of the basic layout according to the fung - shui theories
這個設
計計劃，就是運用這個理論，去設
計一套復式房。設
計的重點是根據風水學的理論做房屋的基本規劃。
I will give you a rough estimate of the costs.
我給你大致估計一下費用。
