誘導外測度 的英文怎麼說
中文拼音 [yòudǎowàicèdù]
誘導外測度
英文
induced outer measure- 誘 : 動詞1. (誘導) guide; lead; induce 2. (使用手段引人隨從自己的意願) lure; seduce; entice
- 導 : 動詞1. (引導) lead; guide 2. (傳導) transmit; conduct 3. (開導) instruct; teach; give guidance to
- 外 : Ⅰ名詞1 (外面) outside; external side 2 (外國) foreign country 3 (以外) besides; beyond; in ...
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 度 : 度動詞[書面語] (推測; 估計) surmise; estimate
- 誘導 : guide; lead; induce; guidance; induction
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Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Aiming at the present design fashion of domestic bigger tunnel, simulating a bigger tunnel with catholic characteristic, confirming the parameters of traffic inducement and controlling ( tunnel length, traffic, the selection and location of detection equipments, amount of roadway indicative lamp, etc ) on the base of the analog tunnel, discussing the controlling and revulsive mode of tunnel. briefly discussing the constitution of tunnel surveillance and controlling system and the executive means of traffic controlling and inducement subsystem and network structure of tunnel controlling system. finally discussing the conformation of emulational tunnel ' s database and detailed executive program by programming the computer emulation of controlling induce subsystem
論文圍繞「交通誘導與控制」這一中心展開,探討長大隧道交通誘導與控制設備及其控制誘導方式;結合國內外對交通流模型研究的成果,提出一套適合長大隧道交通流特點的交通流模型;並針對目前國內長大隧道的普遍設計方式,模擬一個帶有普遍性特徵的長大隧道,確定了關于交通誘導與控制方面的參數(隧道長度、交通量、檢測設備的選取和位置、車道指示燈的數目等) ;以此模擬隧道為基礎,進一步探討隧道的具體控制與誘導方式;簡要探討隧道監控系統的構成、交通控制與誘導子系統的實現方式,隧道控制系統的網路架構;最後通過編程實現控制與誘導子系統的計算機模擬,討論關于模擬隧道數據庫的構建,具體編程實現等。The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體,然後導入畢赤酵母( p8chianastoris )菌株gslls細胞中,在甲醇的誘導下,經過酵母高密度發酵進行pap的表達,經sds page分析,結果表明,在培養基上清液中含有一明顯的特異性蛋臼條帶,大小為34ku ,經western blotting分析,該蛋白與法國pap抗血清有特異性反應,體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性,說明該pap基因在畢赤酵母gs中也得到了正確表達。In natural seawater, the optimum k + is + 40mm tea ( tetraethylammonium chloride ), a potassium channel blocker, cannot inhibit the s. canopus larval settlement and metamorphosis induced by k + - elevated seawater. this appears possible that potassium channels mediate the induction of settlement and metamorphosis by k + in s. canopus larvae, but that k + conductance through these channels is insensitive to external tea
K +通道的阻遏劑tea ( tetraethylammoniumchloride )不能影響由高濃度k +誘導的冠瘤海鞘幼體的附著變態,因此猜測誘導冠瘤海鞘幼體附著和變態的k +通道屬于對外源tea無敏感性的那種類型。Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching
第一部分:等離子點ph ( 5 . 4 )條件下,用平衡透析法和紫外光譜,熒光光譜,共振散射光譜研究了ag ( )與人血清白蛋白( humanserumalbumin ,簡稱hsa )或牛血清白蛋白( bovineserumalbumin ,簡稱bsa )的結合。 scatchard圖分析表明, ag ( )在hsa或bsa中有強弱兩類結合部位,通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag ( )與hsa或bsa的結合存在滯後效應,表明ag ( )與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化( a - b ) ,測得並討論了這一構象變化的速度常數和活化參數。2 hybridization results show that hag1 is specifically expressed in stamens and carpels in vivo ; hag1 mrna was first detected in cultured explants at day 5 in the medium containing high levels of cytokinin and auxin, which could induce floral regeneration in vitro
Rna分子雜交結果表明: hag1特異性地在植株上花的雄蕊和心皮中表達:在高濃度激素培養誘導花芽形成過程中,第5天開始檢測到外植體中有該基因的mrna 。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。分享友人