誘導性表達 的英文怎麼說

中文拼音 [yòudǎoxìngbiǎo]
誘導性表達 英文
inducible expression
  • : 動詞1. (誘導) guide; lead; induce 2. (使用手段引人隨從自己的意願) lure; seduce; entice
  • : 動詞1. (引導) lead; guide 2. (傳導) transmit; conduct 3. (開導) instruct; teach; give guidance to
  • : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
  • : Ⅰ名詞1 (外面;外表) outside; surface; external 2 (中表親戚) the relationship between the child...
  • : Ⅰ動詞1 (暢通) extend 2 (達到) reach; attain; amount to 3 (通曉; 明白) understand thoroughly...
  • 誘導 : guide; lead; induce; guidance; induction
  • 表達 : deliver; express; show; voice; convey; communicate
  1. The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins, gtx were detected with fluorescence microscope, and quantitatively measured with image - pro plus software

    ,抗細胞發出較強的綠色熒光,明重組質粒pegfp - c - fos在biu - 87細胞中成功
  2. We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system

    曰補體受體及其相關分子在modc上的不同分化階段的單核細胞衍生dc ( monocytes一deriveddc , modc )的:將新鮮分離的單核細胞mo ,在含有gm一csf和工l一4培養體系中5一7d ,即分化為未成熟modc ;對培養至sd的未成熟modc ,用tnfa刺激zd ,即分化為成熟modc ;此時再用lps刺激24h ,即為活化的modc 。
  3. Notably, a bile canaliculus was observed in the intercellular space of adjacent two cells. molecular markers, such as ck 19, cd 45, ck 14 and oc. 10, fail to be detected in the first five days of differentiation, while the expression of ov6 and c - met last even ten days later

    免疫細胞化學實驗明,后的細胞成熟肝細胞功能的分子標志,如白蛋白、 aldolaseb 、 apoa4 、李文林博士論文小鼠肝幹細胞系的建立及其生物學特研究apob 、 ao日等。
  4. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因,可負調控指數生長後期的一些代謝途徑,包括糖原的生物合成、糖原的分解代謝、糖異生,而對糖酵解的一些重要基因的則執行正調控功能, csra也調控直接參與pep代謝的三個酶的活水平。
  5. Protective immunity to schistosoma japonicum elicited by co - immunization of cathepsin b dna vaccine with eukaryotic plasmid encoding il

    4真核質粒聯合免疫小鼠保護的研究
  6. The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated

    Niptg,產物沉澱中於約45kda處顯見高合蛋白帶, westernblot分析明,該蛋白帶可被篩庫血清中特異lge抗體識別;而載體本身的26gst蛋白帶則否。
  7. And ha molecules were synthesized in vitro utilizing excessive active udp - glca and udp - glcnac sugar nucleotide precursors in the presence of a divalent mg2 + or mn2 + ion

    經轉化大腸桿菌dh5a和iptg,提取原生質膜在體外利用活前驅物合成了透明質酸。以s
  8. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗菌落,提取質粒經酶切鑒定、 pcr分析以及確證測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源在99以上。將重組質粒pgem - 3abc和載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽克隆,用iptg,收集菌液進行sds - page電泳、 westernblotting分析,結果明, 3ab基因在大腸桿菌中成功,其產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽血清識別。經薄層掃描分析,量占總蛋白量的26以上。
  9. According to the analysis and identification of differential displayed proteome, two key enzymes, adp - l - glycerol - d - mannoheptose - 6 - epimerase and mannosyltransferase, were induced in different salt stress and salt shock for rt19, which commonly participate in the biosynthesis of l - glycerol - d - manno - heptose, a major component of lipopolysaccharide

    通過對rt19的鹽激和鹽脅迫蛋白的比較分析,發現:其中12個蛋白在兩種不同的脅迫條件下都可被。進一步證實: rt19應答不同鹽脅迫刺激採取了不同的反應機制,但這些機制存在共,交織成復雜的調控網路。
  10. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論:截短的乙型肝炎面抗原分子的原核和真核』重組質粒成功被構建及分別在人腸桿菌efl得到和存貞核細胞ifj,並檢測劍其產物的抗原特
  11. Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced. pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l. pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6. 0 - 6. 4 ) and its quantity in total mass of secrete protein exceeded 30 %

    Sds - page分析結果明, mut ~ +組菌株在甲醇第四天後pap在培養液中積累量到最高水平,延長培養時間會致產量下降;在10g / l的甲醇濃度下, pap的到最高;培養基ph值在偏酸條件下( 6 . 0 - 6 . 4 ) pap的量都維持在較高的水平。
  12. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的及其對pc12基因工程細胞的影響用本教研室已構建好的質粒pet一gdnf轉化大腸桿菌bl21 ( de3 ) ,經lmmiptogdnf,並在niz氣nta柱上進行純化,稀釋復后,純度90 %以上。
  13. Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids

    Sj43b pgex 6p 1重組質粒的產物的鄉寸和免疫學質鑒定分析為獲得可溶的rsj43b月6gsta蟲合蛋白,對不同iptg劑濃度、溫度和時間等因素對融合蛋白可溶的影響進行了觀察。
  14. The engineered strain ghd - yz26 / e. coli bl21 ( de3 ) was incubated and induced at 30 for 8 hrs. the results show that the target fusion protein is soluble and active, which is about 15 % compared with the total proteins in cells, and the enzymatic activity reaches 5 u / ml that is about 8 folds compared with the activity of strain yz - 26

    Colibl21 ( de3 )中30培養8hrs ,可獲得可溶,融合蛋白的量約占總菌蛋白的15 ,其酶活力為5u ml ,約是原始菌株的d -海因酶觀活的8倍。
  15. The engineered strain ehd - yz26 / e. coli bl21 ( de3 ) was incubated at 30 for 10 hrs without adding any inducers. the results show that the target product is soluble and active, which account for about 20 % compared with the total proteins in cells, and the enzymatic activity reaches 13 u / ml that is about 13 folds compared with the activity of strain yz - 26

    Colibl21 ( de3 )中30生長10hrs ,在不需添加任何劑的情況下可獲得可溶,目的蛋白的量約占總菌蛋白的20 ,其酶活力為13u ml ,約是原始菌株的d -海因酶觀活的13倍。
  16. In this study we constructed two promoters ( dgp1 and sigp1 ) and analyzed their function in transgenic tobacc os. we combined the dehydration responsive element ( dre ) with guard cell specific element ( gcse ) to construct a novel drought - induced guard cell - specific promoter - dgp1

    受乾旱的保衛細胞特異啟動子( dgp1 , droughtinducedguardcellspecificpromoter )的構建:利用pcr的方法,擴增出乾旱應答元件和保衛細胞特異元件,然後組裝而成。
  17. Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene, and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector. the positive pbv220 - endostatin was transformed into dh5a, and expressed mouse endostatin protein successfully. this study is the basis of the endostatin gene - engineering production

    本文成功克隆鼠內皮抑素基因,構建了內皮抑素基因的原核載體pbv220一endostatin ,該載體為非融合載體,將pbv22o一endostatin成功轉化到大腸桿菌菌dh5a中,並了鼠內皮抑素蛋白,為基因工程方法生產內皮抑素奠定一定基礎。
  18. By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount

    利用基因融合技術,原核並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ,並通過條件的篩選到了可溶與大量純化his - taggedpeac1 - gfp的目的。
  19. Furthermore, suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes, and forward suppression subtractive cdna library of cold - regulated genes was constructed. the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver. subtractive cdna library was differentially screened through cdna macroarray, six hundreds and four cdna clones were identified as cold specifically induced or highly expressed

    ( 5 )應用抑制差減雜交( suppressionsubtractivehybridization , ssh )方法,構建冷的正向抑制差減cdna文庫,低溫處理的幼苗為tester ,常溫處理為driver ,通過cdna微陣列差異篩選cdna文庫,得到604個低溫增強的候選克隆,對其中的84克隆進行dna測序,去除冗餘的cdna ,在genbank中進行核酸和蛋白質同源的比較和功能分析,共有36個單一序列,其中12個cdna在genbank數據庫沒有同源的序列。
  20. Klac gene was ligated to the pet - 30a ( + ) vector for expression. then the recombinant plasmid petlac4 was transformed into e. coli bl21. the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm. sds - page analysis and lactase activity assay showed that klac gene was expressed in e. coli, and that the expression level at 28 was much higher than that at 37

    產物的sds - page分析和酶活測定明, klac基因在大腸桿菌中獲得活,其中低溫條件28下的水平明顯高於37 , 28的細胞經超聲波破碎所測酶活力為0 . 475u / ml , 37僅為0 . 134u / ml 。
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