誘導段 的英文怎麼說
中文拼音 [yòudǎoduàn]
誘導段
英文
induced flow-
We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system
曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。Improvement of shoot - tip micrografting through adventitious bud induction from citrus stem explants
利用柑橘莖段誘導不定芽改進微芽嫁接技術的研究In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。Larvae of s. canopus develop competence to initiate settlement and metamorphosis at least 3 hrs after hatching. but if not any induction, s. canopus larvae can retain a stage of plankton at least 3 - 4 days. 2
在孵化出后3h ，冠瘤海鞘才開始具有附著變態的能力，但在沒有任何誘導物的情況下，有的幼體能保持浮遊幼體階段長達3 - 4天。We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc
我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。But cluster buds can not be obtained from the callus of stems and embryo. it has likely relate to the inner structure of callus. lt is very difficult to get embryoid from mature embryo and its inducing rate is very low, and only 11. 5 % in the media containing n6 + ba 1. 5mg / l + naa 0. 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture
而莖段和胚誘導的愈傷組織基本上沒有叢生芽發生，這可能和不同愈傷組織的內部結構有關。成熟胚的胚狀體誘導率較低，誘導相當困難，在n6 baiThis time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Establishment of the model of cocaine - indued reproductive system injury and cocaine - induced apoptosis of germ cell in different age rat
可卡因誘導不同年齡段大鼠生殖系統損害模型的建立及生精細胞凋亡狀況的研究Abstract : in this paper, traffic data collection techniques for traffic flow guidance systems are analyzed. a modified licence plate recognition method, which is suitable for travel - time investigation in busy streets, is proposed
文摘:討論了交通流誘導系統的交通流量、行程時間和行程速度的採集技術，提出了一種改進的牌照識別法，該方法適合於交通流密集路段上的交通數據採集。The teaching of pass can be conducted in two stages, the first focusing on reducing difficulty by means of simplifying requirements, setting restrictions and taking warm - up exercise, the second focusing on improving students ' proprioceptive sense and controlling ability
摘要將排球傳球教學分為兩個階段：第一階段採用簡化條件、限制性、誘導性等練習，降低練習難度；第二階段注重增強本體感覺、控球能力的練習。Alleged heuristic education, it is the intellectual level according to education purpose, content, student and intellectual rule, use all sorts of teaching methods, use inspire revulsive method to impart ability of knowledge, education, make the pupil learns active and actively, develop in order to promote body and mind
所謂啟發式教學，就是根據教學目的、內容、學生的知識水平和知識規律，運用各種教學手段，採用啟發誘導辦法傳授知識、培養能力，使學生積極主動地學習，以促進身心發展。Rate of cluster buds induced by different gingkgo explants is not the same. among them, cluster buds can be obtained directly from stems and mostly from axillar bud, but the amount is little and differentiation rate is only 20 %
銀杏不同外植體對叢生芽的誘導不同，銀杏莖段培養可以直接得到叢生芽，但數量極少，而且大部分屬于腋芽萌發，分化率20左右。It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。The works we have done as below : the design of whole structure of the non - confocal lif detection system ; the design of the device to couple excitated light into the smf, transport 532nm and 635nm laser direct to the detection area ; designing the device to divide, filter and detect the fluorescence signal
本論文研究這種微流控生化分析系統的激光誘導熒光檢測部分。作為該系統的第一階段研究工作，本課題進行了以下工作： 1非共焦型lif檢測系統的總體結構設計。The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )
經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。These findings of the specific ctls epitopes in irbp and pedf may lead to improved understanding of the pathogenesis of uveitis. our study also provides a strategy to identify specific ctls epitopes within tumour antigen, which is helpful to make tumour vaccine for the patients
本研究也為尋找腫瘤抗原中具有誘導特異性ctls能力的肽片段提供了一種策略，為制備肽片段腫瘤疫苗提供了理論基礎。So, the rank of inducing callus from easy to hard is cotyledon, hypocotyls, stem, leafstalk, radicel, endosperm, leaf and root. 3
誘導的難易分別為：根段、葉片、胚乳、胚根、葉柄、莖段、胚軸、子葉。Calli were induced from internode segments of the methionine resistant plantlets of astragalus melilotoides. the highest yield of protoplasts ( 2. 1 x 106 / g f. wt. ) was obtained from 8 - day - old friable calli after subcultured on fresh medium
用草木樨狀黃芪甲硫氨酸抗性變異系植株莖切段誘導的松軟愈傷組織為材料，通過酶法分離出大量有活力的原生質體。Holding a " zero - sum " logic in mind, they would not offer any inducement and even would refuse to bargain with the enemy, and diplomatic efforts would focus on establishing international coalitions for isolating instead of communicating with its enemy. they would heavily capitalize on coercive means to compel the enemy to comply with its demands
危機期間一味使用強制性手段迫使對手接受己方提出的條件，不給對手任何承諾和利益誘導，甚至不惜發出最後通牒；討價還價和外交努力的著眼點在於獲得國際和國內支持，徹底孤立對手；一旦對手拒絕服從美方要求，美國則很可能憑借己方的絕對優勢訴諸武力解決危機。分享友人