載體噬菌體 的英文怎麼說
中文拼音 [zǎitǐshìjūntǐ]
載體噬菌體
英文
carrier phage-
High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上,使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。To further enhance protein productivity of expression vectors, two artificial transcription activating domains, ah and vp2, were linked to cl represser protein of phage through a soft linker, respectively, and thus two artificial transcription activators were created
為了進一步提高表達載體表達外源蛋白的能力,我們把兩個人工轉錄激活結構域ah和vp2分別通過一個柔性的linker融合到噬菌體ci蛋白的c末端,構建了兩個人工轉錄激活因子。Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer
運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較,從中篩選13段顯示表位特徵的氨基酸殘基序列,用pcr技術擴增相應的核苷酸片段,將其插入到噬菌體表達載體m13ke ,結果預測的13個表位可在噬菌體表面得以展示。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術,從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ,克隆抗體重鏈和輕鏈可變區基因,加入連接肽引物( linkerprimer )組裝成單鏈抗體scfv ( singlechainfragmentvariable )基因並用rs引物進行擴增, sfi 、 not酶切,回收后與pcantab5e載體相連,轉化e . colitg1宿主菌,構建單鏈抗體文庫。In genetic engneering, nonviral dna can be inserted into a phage, which is then used as a cloning vector
在基因工程中,沒有病毒的dna可以被插入到噬菌體中,用作克隆載體。分享友人