載體鏈 的英文怎麼說
中文拼音 [zǎitǐliàn]
載體鏈
英文
chain of carriers-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed. 3
通過合成六條長鏈引物，經兩次pcr擴增，獲得了238bp的植物防禦素afp基因全長，並構建了植物表達載體peafp 。Early last year, articles extolling the virtues of the new economy abounded as " techno - mania " gripped global equity markets. much has changed since then : following the subsequent plummeting of the tech - heavy nasdaq index, the average american has seen his net worth decline for the first time in more than fifty years. the network economy is not the end of history
網路經濟並沒有從根本上改變實物經濟的運行規則，但是，在網路這個載體上，實物經濟的增值鏈被鏡象到網路上形成虛擬的增值鏈，而整個增值過程依靠電子信息流來連接，它的好處是使增值的成本下降、速度加快、范圍擴大。To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces
為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。The electrons are transferred along the chain to a carrier molecule ( coenzyme q ), and then in sequence to a series of cytochromes, finally acting with the enzyme cytochrome oxidase to reduce an oxygen atom, which combines with two h + ions to form water
電子沿呼吸鏈傳遞到載體分子? ?輔酶q上，然後依次經過一系列細胞色素分子的傳遞，最後與細胞色素氧化酶反應，氧原子結合兩個氫形成水從而被還原。The competition in automobile industries has shifted from technology and quality to customer services. distribution channels modes is becoming an important factor in competition. the relationship between the manufacturers and the distributors and the clients has become inseparable
汽車行業的競爭已由技術競爭、質量競爭進入服務競爭階段，作為服務的載體和銷售鏈的終端，分銷渠道的建設已成為汽車企業競爭優勢的重要來源，決勝在於終端已成為各汽車公司的共識。This vector, including sbe2b gene, was 6. 06kb long, containing endosperm specific promoter and a intron that enhanced the transcript efficiency. both sense and antisense cdna were transformed to upregulate and downregulate amylose content and relative branching characters
正向連接的表達載體用於增加胚乳中支鏈澱粉的含量，反向連接用於減少胚乳中支鏈澱粉的含量，增加直鏈澱粉的含量。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Fluorescein and porphyrin have been linked by an alkyl chain, and been firstly used as a fluorescence carrier of opto - chemical sensors. based on the principle of energy transfer, it examined the iodine in the aqueous solution with high sensitivity and wide detection range
通過脂肪鏈把熒光素和卟啉連接起來，並首次把它作為熒光載體應用於光化學傳感器的研製，基於分子內能量轉移原理檢測了水溶液中的單質碘，該傳感器靈敏度高，檢測范圍寬。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。It ' s a modern manufacturer engaged in production of various of door, established in 2001, after years ' development and making progress, with philosophy of “ as the guide of market ”, and under the guide of science and technology, with the theory of talent is the carriers, we specialize in making the unstandard door, steel enter - door, anti - theft door, anti - theft window, unstandard hinge and etc
企業創辦於2001年，經過幾年的開拓進取，本著以市場為導向，以科技為先導，以人才為載體的企業理念，專業生產非標，鋼質進戶門，防盜門、防盜窗、非標專用鉸鏈等。In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226
將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。Firstly, a vector pllz1112 for disruption of sc6a9. 34 and a vector phz1102 containing the whole sc6a9. 34 were constructed
首先，構建了用於sc6a9 . 34的基因中斷載體phz1112和包括了完整sc6a9 . 34並能在鏈黴菌中自主復制的載體dhz1102 。At last, hau3r gene was inserted downstream of ptipa of pij6021, a streptomyces expression vector
將hau3 ~ r基因克隆到鏈黴菌表達載體pij6021的ptipa下游，獲得重組子phz2085 。They found that np30 had partial cross - reaction with sea and maa and could be used as " antigen reagent " in the immunodiagnostic assays based on antibody detection of schistosomasis japonica. it is difficult to obtian gaa by genetic engineering methods, because gaa is glycoprotein
基固，擴增產物鑒定后，將其克隆apuc19載體，重組子用san驢r 』 s雙脫氧鏈終止法和自動測序法測定其序列，序列與genebank中及已發表的抗體序列進行比較分析。Strain sa - coo by southern hybridization. a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357, a streptomyces - e. coli bifunctional vector carrying two cohesive sites
為了獲得膽固醇氧化酶基因，以大腸桿菌-鏈黴菌雙功能柯斯質粒phz1357為載體，構建了灰色鏈黴菌atcc14811的基因組文庫。Logistics delivery enterprise play the important role in the side of the cost control and providing rapid and accurate service as the carrier of material flowing, the problem of the size of the logistics delivery truck fleet come out
物流配送企業作為供應鏈物資流動的載體和組織管理者，在供應鏈成本控制和提供快速準確服務方面發揮著重要作用，根據客戶需求組建一支何等規模的配送車隊的問題也就隨之產生了。分享友人