轉亞氨基酶 的英文怎麼說

中文拼音 [zhuǎnān]
轉亞氨基酶 英文
transaminase
  • : 轉構詞成分。
  • : 名詞[化學] (氮和氫的化合物) ammonia; hydrogen nitride
  • : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
  • 氨基 : amido group
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,化大腸桿菌dh5菌株,篩選芐青霉素抗性菌落,提取質粒經切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,克隆3abc因至原核表達載體ptriex - 4neo中,通過切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc因708bp處出現了17bp的缺失,碰巧在3ab因后形成一終止密碼子,但3ab因的閱讀框架完整,選出含有3ab因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
  2. The a - transglucosidase was selectively modified by pcmb, me, edc, clac, acetyl acetone and nbs, and changes in the activities of the enzyme have been detected. the reaction of a - transglucosidase with pcmb, me, edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. the acetyl acetone and nbs were found without inhibition effect

    酚丙酮、小涅代唬拍酷胺剛b引等化學修飾劑對a葡萄糖的幾種酸殘進行選擇修飾,並測定其活力變化,結果表明: pcmb 、 me 、 edc 、 ciac能顯著抑制的活性,活力的下降與修飾劑的濃度有關,乙酚丙酮、 nbs白巾飾對的奪製作用不明顯。
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