轉座因子 的英文怎麼說
中文拼音 [zhuǎnzuòyīnzi]
轉座因子
英文
transposable element-
Bacterial transposons carry genes encoding antibiotic resistance.
細菌轉座子可以攜帶對抗生素的抗性基因。Advances in retrotransposon in animal genome
動物基因組中反轉錄轉座子的研究進展Further, their luciferase activities were determined in ty medium, and showed that tn5 - 1063 was inserted directly into loci downstream of promoters in 042bm genome
它們都表現發光酶活性,表明轉座子正向插入到基因組中的某個啟動子下游。Cloning and sequencing of the fragment of human endostatin gene
來自中間偃麥草基因組的類反轉錄轉座子片段的克隆及其特徵分析Rna silencing is a common phenomenon of rna degradation that is induced by homologous sequences. virus and transposon invasions and various kinds of aberrant rnas can provoke rna silencing
Rna沉默是生物體中普遍存在的一種由同源序列引起的rna降解過程,病毒或轉座子入侵、以及體內產生的各種異常結構rna都可能成為誘導rna沉默發生的因素。A new resolution vector based on tnpi - mediated site - specific recombination system of b. thuringiensis transposon tn4430 was developed. the crylac10 or other target dna, and the gene ori1030, from a plasmid of wide type b. thitringiensis subsp
利用轉座因子構建解離載體的可行性利用蘇雲金芽胞桿菌轉座子tn4430的解離區構建了解離載體。This paper summarizes the commonly used methods for the research of gene function of filamentous fungi, such as transposon tagging, gene knockout, rna interference, over - expression and yeast hybrid system, and provides a discussion on the advantages and disadvantages of those methods
本文總結了目前絲狀真菌基因功能研究中常用的方法,如轉座子標簽法、基因敲除技術、 rna干擾、超表達及酵母雜交系統等,並對各方法在絲狀真菌研究中的優缺點進行了闡述。Cloning and sequencing of a retrotransposon gene fragment from eggplant
茄子反轉錄轉座子基因片段的克隆及序列分析In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。Based on the blast analysis and other studies, osddl mutant was a multi - copy - insertion mutant, and one of the insertion sites was in an nptii like transposase gene, whereas osdd2, a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor
Osdd1突變體可能是多拷貝插入,其中一個插入到nptii轉座酶類似基因。 osdd2突變體為單拷貝、插入到一個wrky類轉錄因子基因5翻譯起始區附近。The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變,獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較,發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接,獲得亞克隆pgxn201c ,對pgxn201c進行測序分析,發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。Some important and creative conclusions are achieved from study as follow : 1. through a lot of experiments, we find out the effect between two rotors " unbalanced vibrations is weak, so we can get four inner rotor ' s amplitude value from left and right bearings by trial - weight twice, and get four external rotor ' s amplitude value from left and right bearings by trial - weight twice. it is said that when get eight amplitude value and effect coefficient by trial - weight four times, we can deal with whole machine balancing for dual - rotor system with little rotating speed difference
研究發現微速差雙轉子系統內、外轉子不平衡量之間的相互影響很小,因此可以對內轉子兩次試重,從左右軸承座上獲得4個對應于內轉子振動值;對外轉子兩次試重,從左右軸承座上獲得4個對應于外轉子振動值,即通過四次試重,獲得8個振動值, 8個影響系數,就可解決該類轉子的動平衡問題。分享友人