轉染 的英文怎麼說

中文拼音 [zhuǎnrǎn]
轉染 英文
transfection
  • 轉 : 轉構詞成分。
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Stable suppression of afp gel expression by rnai in smmc - 7721 cells

    表達質粒穩定轉染肝癌細胞克隆的構建
  3. The protective effect of bcl - 2 gene transfection on heat stressed cardiomyocytes

    2基因轉染對熱應激心肌細胞的保護作用
  4. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  5. 17 - hsd8 / pcdna3. 1 recombinant protein expressed in hek 293 cells and it can slightly interconvert estrone to estradiol. moreover, it can catalyze the conversion from testosterone to androstenedione. however, 17 - hsd3 did n ' t show any conversion to all the substrates checked in this study

    而17p一hsds轉染hek293細胞表達的17p一hsds / pcdna3 . 1重組蛋白能微弱地催化el和ez之間的相互轉化，同時也能催化從t到a的氧化反應。
  6. Protective effect of c - fos antisense gene recombinant transfection on ischemic hypoxic cardiomyocytes

    反義基因轉染對缺血缺氧心肌細胞的保護作用
  7. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  8. The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus

    另外，通過共轉染和篩選純化獲得了攜帶appa基因的重組家蠶桿狀病毒。
  9. The primers were in the sequence of vector pegfp - 1 outside the lox sites, and the whole sequence of the vector were transfected into the fibroblast cells

    引物設計的位置是在loxp和lox511之外的載體序列，整個載體序列都隨轉染而進入細胞。
  10. The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc

    當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。
  11. The binding characteristics of the opioid ligand

    受體激動劑對穩定轉染
  12. Transfection efficiency comparison of cationic liposome - dna complexes and lipid - protamine - dna complexes in vitro

    復合物體外細胞轉染率比較
  13. We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker

    在實驗中同時共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
  14. They are combination of co - transfection with rhe and gfp expressing plasmids, co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time. whichever the combination may be, we always observed the phenomena of chromatin bridge in co - transfected cells. these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits

    這時，存在三種可能的情況，即共轉染rhe和pedna3 . 1 * kc 、共轉染rhc和pedna3 . 1 + kg 、共轉染rhe和rhc和pc洲a3 . 1 + kg ，但都觀察到明顯的染色質橋現象，進一步證明這種表型是人集縮素中smc亞基的缺陷的特徵表型。
  15. 3. polyethylenimine ( pei ), a water - soluble cationic polyelectrolyte, has been extensively used as a deformable and mobile substrate for a biomembrane for it high transfection efficiency of chemical - based delivery systems. what ' s more, pei is good buffer in physiological environment ( ph < 9 )

    3 、聚乙烯亞胺（ pei ）是一種陽離子型的聚合物，由於它在動植物細胞內優良的轉染效率，而被廣泛的應用，並且在生理環境下（ ph 9 ）它具有很強的緩沖能力。
  16. Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro

    體外成功地完成了cd14 +單核細胞來源的樹突狀細胞（ dc ）的培養，並進一步研究人癌胚抗原重組痘苗病毒（ rv - cea ）轉染dc后體外誘導的cea特異性細胞免疫。
  17. Conclusion : dendritic cells ( dcs ) can be derived from peripheral blood monocytes in vitro successfully treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml. second, dcs from the patients of advanced carcinoma of large intestine were transfected with rv - cea, and then cocultured with autologous t cells. the cytotoxic activity against cea - secreting tumor cells were assessed and compared with those of t cells induced by dcs without rv - cea transfection

    其次，用rv - cea轉染晚期大腸癌患者的dc ，再激發自體t細胞，然後檢測t細胞對cea分泌性腫瘤細胞的體外殺傷活性，並與未經rv - cea轉染的dc激發的t細胞及野生型痘苗病毒轉染的dc激發的t細胞進行對比。
  18. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  19. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  20. Transfection of cell lines impaired for vzv infection with a plasmid expressing human ide resulted in increased entry and enhanced infection with cell - free and cell - associated virus

    Vzv感染受抑制的細胞系通過質粒表達轉染人類ide蛋白后可增加游離病毒顆粒以及細胞內病毒引起的感染。
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