轉錄弱化子 的英文怎麼說
中文拼音 [zhuǎnlùruòhuàzi]
轉錄弱化子
英文
transcriptional attenuator- 轉 : 轉構詞成分。
- 錄 : Ⅰ名1 (用做記載物的名稱) record; register; collection; selections 2 (姓氏) a surname Ⅱ動詞1 (...
- 弱 : Ⅰ形容詞1 (氣力小; 勢力差) weak; feeble 2 (年幼) young 3 (差; 不如) inferior 4 (接在分數或...
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 轉錄 : [電學] transcribe; transfer; [聲學] mixing; rerecording; [生物學] transcription; duplicating轉錄...
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
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