轉錄本 的英文怎麼說
中文拼音 [zhuǎnlùběn]
轉錄本
英文
transcript-
Dreb - type transcription factors can accept stress signals from environment and promote the expression of stress - tolerant genes. four dreb homolog genes, gmdreba, gmdrebb, gmdrebc and osdreb4 - 2 were isolated from soybean [ glycine max ( l. ) merr. ]
Dreb類轉錄因子能接受逆境信號並啟動逆境應答基因的表達。在本研究中，以大豆和水稻為材料，共克隆了4個dreb的同源基因，分別命名為gmdreba ， gmdrebb ， gmdrebc和osdreb4 - 2 。Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。From these results, it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region, but not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase iii transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rnapolymeraes
本實驗為rna聚合酶在真核生物細胞核中的轉錄位點提供了較為直接的證據，這對人們進一步了解rna聚合酶的轉錄機制、加工和運輸過程及三種rna聚合酶之間的結構與功能關系具有重要的意義。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。Their originally developed " super mpeg - 2 pre post processor " enables high quality picture recording playback, and equipped with their " intelligent 2 pass encode " technology, high picture quality dubbing from hdd to dvd is possible. mr. namiki explained that jvc will continue to appeal technology in their dvd recorders with the " 3 - in - 1 function, video quality, and easy use " points in developing their dvd recorders. cyberlink, inc
他對使用自行開發的" super mpeg - 2 encode pre post processor "進行錄像和播放時的高畫質技術進行了說明，還介紹了日本victor株式會社正在開發的著眼于"三合一功能畫質方便性"的dvd錄像機，該錄像機採用" intelligent 2 bus encode "技術，以使hdd能向dvd進行高畫質轉錄的這些技術為基礎。Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas. its latex is the unique source of crude rubber used in current industry. because of its special and important use, the rubber tree has been extensively planted in tropical areas. increase production is always the main target in rubber tree cultivation. since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant, not only the latex production had been increased largely, but also a new set of rubber tapping system had been established, leading to a series of economic benefit. owing to ethrel " s extensive application, its side effects had been found more and more obviously, such as tapping dry, speeding up senescence, shortening the life span of rubber tree etc. in order to overcome the side effects and increase production more availably, for a long time, people had carried out lots of research work on cell level, membrane level, physiology and biochemistry of laticifer contents. but the mech anism why ethrel increased latex production was not yet understood completely. this study had cloned the ethylene receptor gene ( efrl ) from rubber tree, and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level. the results were as follows : 1
但是，由於乙烯利應用的普及，乙烯利刺激割膠引起橡膠樹發生死皮病及加速膠樹衰老，縮短膠樹壽命等副作用也越來越明顯。為了克服這些副作用，使乙烯利能更有效地刺激增產，長期以來，人們在細胞水平、膜水平和乳管細胞內含物的生理生化層面上進行了大量的研究，但仍未完全了解乙烯對膠樹的作用機制。本研究從分子水平入手，克隆橡膠樹的乙烯受體基因（ etr1 ） ，並在轉錄水平和蛋白質翻譯水平上研究etr1基因在乳管細胞中的表達及與乙烯刺激的關系，取得了以下結果： 1Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。The sequence of ck2 a from lily pollen have been deposited in the genebank datebase ( accession number, af517838 ) by pcr, we also proved the persence of two transcripts in germinated lily pollen
通過使用兩對不同引物進行pcr反應還發現在萌發的百合花粉中存在兩個ck2的轉錄本。Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified
為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。The centre for health protection of the department of health today november 18 received a report from the university of hong kong that laboratory tests using reverse transcription polymerase chain reaction rt - pcr method on three out of the seven specimens taken from patients of the caritas medical centre outbreak yielded positive results to parainfluenza virus, but all the seven specimens were negative for other common respiratory pathogens
生署生防護中心今日十一月十八日收到香港大學的報告，明愛醫院病童的七個樣本經大學完成反轉錄-聚合鏈反應rt - pcr測試后，其中三個樣本證實對副流感病毒呈陽性反應，但是所有七個樣本均對其他常見的呼吸道感染病原體呈陰性反應。The technology and methods of the study on molecular mechanism of cytoplasm male sterility ( cms ) are introduced in regard to mitochondrial genome, mitochondrial gene, mitochondrial rna, mitochondrial protein, transformed plants and abortion of pollen
摘要本文從線粒體基因組、線粒體基因、線粒體轉錄rna 、線粒體蛋白、轉基因植物以及花粉敗育機理六個方面詳細介紹了植物細胞質雄性不育分子生物學研究的技術和方法。The main difference between the two transcripts is lie in the 5 ' - untranslated region. one is 62bp larger than the other. this region may be jp related to the regulation of protein translation.
它們不同之處主要是在5非翻譯區，一個轉錄本在5非翻譯區比另一個多62bp ，推測此區域可能與ck2翻譯水平上的調控有關。These alleles showed wg - like phenotype ( notch et al. ), the germline clone embryo showed cuticle fusion phenotype
這些突變或是無義突變（ b158和b140 ）或是破壞原初轉錄本剪接的突變（ b173 ） 。The bands of ~ 6. 0kb and ~ 7. 5kb are the novel isofoms that have n ' t been cloned
Okb和刁skb的條帶可能是新的hse基因的轉錄本。Our research was mainly focused on the transcription pattern of xist in preimplantation mouse embryos.
但是由於胚胎發育早期xist轉錄本數量的稀少而難以達到很好的效果。The clpg mutation is a a g substitution 32. 8 kb upstream of gtl2, which was later shown to be the only difference between the callipyge and wild - type chromosome in what is probably a long - range control element ( lrce )
2002年， freking等人發現了一個位於gtl2基因上游32 . 8kb的a g的突變，這一突變位於一個新發現的轉錄本clpg1內部，它是callipyge羊和正常羊的唯一不同之處。Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts. the aberrant expression of sh2a gene in some cancers was found. it suggests that sh2a gene relates to tumors
Rtpcr及northern印跡雜交發現sh人在多種組織中有表?二?達，有3個轉錄本，而且在癌及癌旁組織的比較中發現，腫瘤組織中表達較高，初步證明與腫瘤的發生有關。In the 317 unigenes of 592 clones, 42 novel full - length genes and 90 novel testis - specific transcripts were obtained. they were deposited in the genbank. one of testis - specific transcripts, testis - specific calpastatin ( tcast ) and two novel full - length genes, nyd - sp27 and human ubiquitin - like fusion protein ( hufp ), were selected for study
我們對其中的一個睪丸特異的轉錄本一鈣激活的中性蛋白酶古制物（ testi specificcalpastatintcast ） ，和兩個新的全長基因一nd七p27和人泛素樣融合蛋白山umanublqultln likeusionproteinhwp ）進行了進一步研究。分享友人