轉錄組 的英文怎麼說
中文拼音 [zhuǎnlùzǔ]
轉錄組
英文
transcriptome-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。It refers to the release of the viral genome from its protective capsid to enable the nucleic acid to be transported within the cell and transcribed to form new progeny virions
它指的是將病毒基因組從它的保護性衣殼中釋放出來,使核酸能在細胞內轉運並能轉錄以形成新的子代病毒。For functional genomics, huge est databases from multiple tissues of a number of tree species have been rapidly accumulated, and molecular analyses on secondary growth and wood formation, flowering, and cold hardiness have given some insights into the metabolic pathways of those tree - specific development processes
功能基因組學方面,已分析了主要造林樹種多種組織的轉錄組est序列,對林木次生生長與木材形成、開花和抗寒性的形成等過程開展了功能基因組學研究。Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly
結果表明, tn5gusa5插入位點有一定的規律性: tn5gusa5在xcc8004基因組上傾向于插入低gc含量( 50左右的區域插入密度最高)區段;插入位點的靶序列有一定的特異性,在靶序列的首位鳥嘌呤出現的幾率高,而在靶序列的末位胞嘧啶出現幾率高; tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。Seven pairs of degeneracy primers for cloning are schemed out by using bioinformatics software
建立白樺花芽組織rna的逆轉錄體系。Advances in retrotransposon in animal genome
動物基因組中反轉錄轉座子的研究進展In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應( rt - pcr )獲得兩株新城疫病毒( ndv )血凝素-神經氨酸酶( hn )基因cdna ,然後定向插入真核表達質粒pcdna3中,構建了含hn基因的重組質粒。Notch interaction with its ligands induces the cleavage of its intracellular domain ( ic ), and the notch ic translocates to the nucleus and binds to rbp - j, the mammalian homolog of su ( h ), to transactivate transcription of target genes such as e ( spl ) ( enhancer of split ), hesl ( hairy and enhancer of split ) and hes5 four notch receptors and their ligands are differentially and redundantly expressed in a variety of vertebrate tissues
它通過其識別序列( cogtgggaa )結合於受調控基因的啟動子區,在轉錄激活因子的驅動下調節細胞分化和個體發育相關基因的表達。在沒有n 。 tch胞內段的情況下, rbpj可與包含sm盯( silencingmediatorforretlnoldandthyroidhormonereceptor )和組蛋白去乙酞化酶的轉錄輔助抑制復合物結合,當notch信號被激活時; rbpj可與n 。It is inferred that its active transcription occurs in the same region, not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rna polymeraes
這一結果不僅直觀地向人們表明了rna聚合酶在真核細胞核中的轉錄位點,而且對於人們進一步認識和理解rna聚合酶的轉錄機制、其轉錄產物的加工運輸途徑、以及真核細胞當中不同的rna聚合酶間的組織和調控關系都將有著重要的理論意義。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。K01458, 1284bp ), we designed the primers and regard the pcr productions as the internal standard. then by using rt - pcr analysis, high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected, low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard
K01458 , 1284bp )設計引物,以其pcr產物作為內標,分別以鴯鶓各個組織反轉錄產物為模板,通過多重pcr的檢測ghrelin的mrna在鴯鶓的各個組織中表達情況,結果發現ghrelin的mrna在鴯鶓ghrelin的mrna除在腺胃特異表達外,在肺、肌肉、回腸、十二指腸、紋狀體、小腦、腦干、肌胃也有少量表達。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上,根據國內外已發表的ibv基因序列,分別設計特異性引物,應用不同引物進行反轉錄合成cdna ,分片段對ibv的主要結構基因進行pcr擴增,並分別將各個目的片段克隆到puc19載體上,在大腸桿菌dh5中實現目的基因的分子克隆,經藍白斑篩選、限制性內切酶分析、 pcr鑒定,篩選出重組陽性質粒,並對各個目的基因片段進行序列測定,從而獲得ibv主要結構基因全序列。Zygotic genome activation ( zga ) is a nuclear reprogramming event that transforms the genome from transcriptional quiescence at fertilization to robust transcriptional activity shortly thereafter
合子基因組激活( zga )是一個細胞核重編程事件,使基因組從受精時的轉錄靜止轉變為立即的活躍轉錄。As a member of an important transcription factor family, ap - 2 a expresses in specific tissue and binds with specific dna sequence
Ap - 2作為一個重要的轉錄因子ap - 2家族的成員,具有組織表達特異性和dna結合特異性。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively
提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄,剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3 - dctp or cy5 - dctp for the experimental and control group respectively. the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h
提取as _ 2o _ 3作用k562細胞前後的總rna ,用superscript逆轉錄酶逆轉錄成cdna第一鏈,並在逆轉錄的過程中,用cy3 cy5熒光染料分別標記對照組處理組,與自製的k562細胞基因表達譜晶元雜交。It implies that gene expression in plant cells might have changed under the stimulation of sound wave. thirdly, the cell cycle of protoplast of control group and stimulated group ( stimulating for 9 days ) was estimated by flow cytometer. the results showed that the number of cells of stimulated group decreased in g0 / g1 phase and increased in s phase
圍繞中心法則分別測定了dna 、 rna和可溶蛋白質的含量,發現聲波刺激組的dna含量變化不明顯,而rna和可溶蛋白質的含量都有所升高,且以刺激9天的實驗組最為突出,表明在強聲波的作用下,有關應力響應的轉錄因子被啟動,轉錄水平提高,從而翻譯合成較多的蛋白質,促進植物的生長發育。On the one hand, the theoretics of drug discovery become more comprehensive and more profound as the development of numerous new disciplines such as genomics, proteomics, transcriptomics, metabonomics, bioinformatics and system biology ; on the other hand, the pathways for new drug discovery are broadened by the continuous improvement of technological platforms including computer - aided drug design, high throughput screening, high content screening, biochip, transgenic and rnai technology
一方面,基因組學、蛋白質組學、轉錄組學、代謝組學、生物信息學、系統生物學等新興學科的崛起與發展,為藥物發現提供更為廣泛而深刻的理論基礎;另一方面,計算機輔助藥物設計、高通量篩選、高內涵篩選、生物晶元、轉基因和rna干擾等高新技術的發展和完善,為藥物發現提供了新的技術手段和有力工具,極大地拓寬了藥物發現的途徑。Biomicroarray is an important technique in the medical research at postgenome era, especially in the transcriptomic, proteomic and oncogenomic research
摘要生物晶元是后基因組時代醫學研究中的重要手段,其在轉錄組、蛋白質組與腫瘤功能基因組研究中的作用日益突出。分享友人