連接序列 的英文怎麼說

中文拼音 [liánjiēxùliè]
連接序列 英文
catenation sequence
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 連接 : connect; fit together; link; marry; mate; joint; association trail; linkage; concatenate; concate...
  1. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon

    對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。
  5. Ghrelin regulates pituitary growth hormone ( gh ) secretion. in the study, we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck, goose and emu by using rt - pcr and 3 " - race methods. and then deduced the amino acid sequences of preproghrelin and ghrelin in duck. goose and emu. sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no. ab075215, 836bp ) and proventriculus cdna was performed as the template

    Ab075215 ， 836bp ）設計引物，以鴨、鵝、鴯鶓腺胃cdna為模板，通過rt - pcr及3 - race的方法，將各個產物經過回收，再經連接轉化，克隆測序，拼接后得到鴨、鵝、鴯鶓preproghrelin基因的cdna序列及ghrelin的cdna序列，並由此推測出preproghrelin及ghrelin的氨基酸序列。
  6. The egs can be covalently linked to ml rna, the catalytic rna subunit of rnase p, forming a new kind of ribozyme, m1gs

    將egs共價連接到m1rna的3末端成為附屬于m1rna的一段引導序列，稱為gs 。
  7. In the proposed join algorithms, although some algorithms such as mpmgin algorithm [ 23 ], outperform standard rdbms join algorithms, they perform a lot of unnecessary computation and i / o for matching basic structural relationships, especially in the case of parent - child relationships ; other algorithms such as the stack - tree - desc algorithm [ 24 ], represent the state - of - the - art in structural joins, however, they do not utilize indexed structures but sequentially scan the input lists. thus, i / o ' s can be wasted for scanning element that do not participate in the join, and join speed can be influenced

    另外，在所提出的演算法中，盡管有的演算法，如mpmgjn演算法[ 23 ]優于標準的rdbms連接演算法，但是該演算法為匹配基本的結構關系，特別是在父子關系情況下，執行了大量不必要的計算和佔用了大量的i / o資源；有的演算法雖然代表了結構連接演算法的先進水平，如stack - tree - desc [ 24 ]連接演算法，但是它沒有利用索引結構而是順序瀏覽輸入列表。
  8. The native expressed product from e. coli bl21 ( de3 ) strain, however, showed weak activity against tmv. gp609 and zwemu were inserted into pemu - mcs - n, an expression vector for monocotyledon. and rhxjb was inserted into pkylx71 : 35s2

    將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi （ de3 ）菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。
  9. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。
  10. At first, the text is segmented to words and converted to a sequence of part - of - speech tags ; then based on the pos tags sequence parameters and phrase - break distance information from training, markov model is used to get the most likely phrase break sequence

    首先，文本進行分詞，並轉換為一列由詞性標記所組成的序列；然後使用馬爾可夫模型，利用人工標注數據庫訓練詞語連接處詞性標注序列的概率分佈和連接類型序列的距離信息，得到輸入的詞性標記序列對應的具有最大似然概率的連接類型序列，最後利用后處理規則進行適當的糾錯。
  11. By blue - white screening and sequence analysis, we obtained the positive clone. 2. we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation

    我們從擬南芥基因組中克隆了dreb1c基因全長，並將其連接到中間載體pgem - t - easy上，進行了測序驗證，結果顯示所克隆的dreb1c序列完全正確。
  12. Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid

    Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。
  13. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  14. Message queuing communicates with such queues through a connector application

    消息隊列通過連接器應用程序與這類隊列通訊。
  15. Your implementation of the connections property can return either the list of connections defined in the current web application, or a list of connections defined at a global level, such as a list of data connections maintained by the design environment

    屬性的實現可以返回在當前web應用程序中定義的連接的列表，或是在全局級別上定義的連接的列表（如由設計環境維護的數據連接的列表） 。
  16. We designed a wireless asymmetric physical layer link model with a direct sequence spread spectrum system as a transmitted reference. uplink and downlink are both one - way links connected by an ip tunneling. uplink is consisted with the narrowband radio data link of the warfighter information networks ( win )

    研究了無線非對稱鏈路，提出了一種用參考法直接序列擴頻（ dsss ）實現的無線非對稱數據傳輸的物理層鏈路模型：利用現有戰斗員信息網電臺的窄帶數據鏈作為上行通道，通過ip隧道技術保證單向鏈路的連通性。
  17. Install this update to address an issue on computers running windows 2000 service pack 4 in which clients accessing a print device attached to a print server are no longer able to print when using the lexmark monolithic pcl driver after installing security bulletin ms05 - 043 kb896423

    在運行windows 2000 service pack 4的計算機中，在安裝安全公告ms05 - 043 （ kb896423 ）之後，訪問連接到列印服務器的列印設備且使用lexmark monolithic pcl驅動程序的客戶端將不再能夠進行列印。安裝本更新程序可以解決這一問題。
  18. Base on the encoding sequence of ast7 and gkr ( by which asts were concatenated ), two primers were synthesized and constructed them in a proved pcr method

    根據grb - ast _ 7和gkr （ ast神經肽之間的連接序列）的編碼序列設計合成兩個引物。
  19. Connect tasks and containers in the sequence container using precedence constraints

    使用優先約束連接序列容器中的任務和容器。
  20. The recombinated plasmid was named pu54. based on this material, 4 recombinated plasmids pu54 - a, pu54 - b, pu54 - c and pu54 - d were construted, which contained different cloning segments of ul54 gene. 11 m1gs ribozyme containing different gss designed to the target sequences of ul54 mrna were construted by pcr

    從hcmvdna聚合酶ul54基因序列中搜尋了11個適合gs互補的靶位，人工核酶m1gs轉錄模板dna包含有t7啟動子、 m1rna基因、連接序列以及gs序列四個組成部分。
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