連接的糖基化 的英文怎麼說
中文拼音 [liánjiēdetángjīhuà]
連接的糖基化
英文
n-linked glycosylation- 連 : Ⅰ動詞1 (連接) link; join; connect 2 (連累) involve (in trouble); implicate 3 [方言] (縫) ...
- 接 : Ⅰ動詞1 (靠近;接觸) come into contact with; come close to 2 (連接; 使連接) connect; join; put ...
- 的 : 4次方是 The fourth power of 2 is direction
- 糖 : Ⅰ名詞1 [化學] (碳水化合物) sugar 2 (食糖的統稱) sugar 3 (糖果) sweets; candy; sweety Ⅱ形容...
- 連接 : connect; fit together; link; marry; mate; joint; association trail; linkage; concatenate; concate...
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Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成,當其易位穿過內質網( er )膜后,被gpi :蛋白質轉酰胺基酶( gpit )識別, gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system
動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ,末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變,應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達,測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 , 4 -糖苷鍵連接而成的寡糖,它具有良好的水溶性、組織兼容性,在生物體內易降解,同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能,因此已成為眾多領域的研究熱點。Effect of advanced glycosylation end products on the expression of connective tissue growth factor and fibronectin in cultured human mesangial cells
糖基化終產物對人腎小球系膜細胞結締組織生長因子及纖維連接蛋白基因表達的影響Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后,經瓊脂糖凝膠電泳鑒定后,與pcambia1303連接並轉化大腸桿菌dh5a ,陽性重組子經pcr和限制性內切酶酶切圖譜分析,表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先,以成體牛蛙的腦垂體總rna為模板,進行rt ? pcr擴增得到約660bp的特異性pcr產物帶,切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收,將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后,進行菌落pcr 、質粒酶切鑒定,篩選出陽性菌株,測序結果經blast分析,與已報道的牛蛙生長激素基因高度同源,證實此陽性克隆為牛蛙生長激素基因轉化子,命名為pbfgh 。The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測,將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中,再轉化到jm109感受態細胞,得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆,結果表明,得到的陽性重組子中含有a節段全長基因,插入到載體中的方向正確。These results indicated that chemical treatments may cleavage the linkages between lignin and polysaccharides, and between monomers of lignin, which results in the decrease of compositions of lignin in primary and secondary wall of rice straw, and hence increases the degradation rate of secondary wall and degree of primary wall degradation observed under histological investigation
上述結果表明,化學預處理通過破壞木質素與結構性多糖、木質素單體之間的連接,導致細胞壁各層的木質素組分不同程度的下降是化學預處理后稻草亞細胞結構變化的化學基礎所在。分享友人