連接轉導 的英文怎麼說

中文拼音 [liánjiēzhuǎndǎo]
連接轉導 英文
co transduction
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 轉 : 轉構詞成分。
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 連接 : connect; fit together; link; marry; mate; joint; association trail; linkage; concatenate; concate...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. 7 - 10 days " seedlings after the seeds germinated, the meristem in the stem apex functioned to form true leaf primordium, meanwhile, the vessel elements which differentiated from parenchymatous cells of the middle and upper portion of the cnz connected with the vessel elements from procambium of epcotyl - shoot region

    種子萌發7 - 10天，幼苗的莖端分生組織活動形成真葉雛形，此時由子葉節區中、上部薄壁細胞的轉分化形成的導管分子與上胚軸-苗區原形層形成的導管分子發生連接。
  3. Analysis for magnetic field of srm is very complicated since the flux waveforms in different parts of the motor vary with motor phase, stator pole and rotor number, also with the winding connections, etc, which results in the srm losses, especially iron loss calculation difficult. the temperature analysis due to the losses has become a study hot spot

    Sr電機鐵心的磁通波形不僅與電機的相數有關，還與定、轉子齒極數、定子繞組連接方式等密切相關，從而使得sr電機磁場分析很復雜，復雜的磁通密度波形導致電機損耗特別是鐵損的計算非常困難。電機的損耗又會引起電機的溫升，因此高性能的sr電機的熱分析也成為目前的研究熱點。
  4. As an up - to - date applied tech, ecu hardware - in - loop simulation system ( ecu hilss ) can be regarded as a tool with integrated software and hardware, which is adaptive for real - time simulation. during debugging, the hilss is connected to ecu directly as a virtual engine, receiving the control from the ecu and sending out the homologous running message of the engine, which reduce the tests and cost of all kinds. besides, operators are provided with a convenient way of conditioning and observing the whole system because of its software controlled mechanism of imparting information

    作為一門新興的應用技術， ecu硬體在環模擬系統（ ecuhilss ）可以看成是一套軟硬體集成的實時模擬工具，在對外部ecu系統進行調試時，它作為虛擬發動機的載體直接與ecu系統相連，接受ecu的控制調節，並向ecu提供相應控制下的發動機實時運轉工況信息，這樣就相應地減少了臺架試驗，在節省各種人力物力的同時縮短了調試時間；此外由於hilss是通過編制軟體來實現各種信息傳遞，它能給操作者提供方便友好的調節觀測環境，從而大大增強ecu調試過程的靈活性和可靠性，消除由於某些試驗因素可能導致的危險性。
  5. It is possible to make the strap connecting handle to pod very short, and if the handle is poorly positioned it may get hooked by a steering toggle in flight, causing an accidental deployment

    側置方式能夠是手柄到備份傘包皮之間的連接帶最到最短，需要主意的是，不合適的手柄安裝位置回導致在拉下轉彎剎車的時候碰到手柄，可能導致意外開傘。
  6. In practical application, the m10 180 bolts, which are installed in the head cover of the torque divider of ts5560 special automobile, sometimes fracture all of a sudden. according to the tests of acceleration, stress and strain, and the exciting test, it is resonance vibration that leads to the compound - bending fatigue fracture of the bolts. the exciting force caused by drive system, transmitted through the torque divider, produces a 1000 - 1500hz cyclical excitation at the junction of the bolts and head cover, which causes the first order bending resonance vibration of the bolts

    通過加速度、應力應變測試實驗和激振試驗的研究，發現ts5560特種車分動器取力器氣缸端蓋m10 180螺栓斷裂問題是由於共振引起的雙向彎曲疲勞斷裂：傳動系統工作所產生的激勵，經過分動器結構的傳遞（特別是分動器一階扭轉模態的放大） ，在螺栓的連接處產生1000 1500hz的振動激勵，其導致螺栓一階彎曲共振，在螺栓一階彎曲模態的阻尼比較小的情況下，使得螺栓產生較大的共振響應，導致螺紋處的彎曲動應力較大，疲勞壽命較低。
  7. The sensing chip is connected with special amplification circuit of the transmitter by the conductor, and using piezo - resistance effect of semi - conductor silicon material to realize the transformation between pressure and electric signal

    敏感晶元通過導線與變送器專用放大電路相連接。它利用半導體硅材料的壓阻效應，實現壓力與電信號的轉換。
  8. After digested with ecori and noti, the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc. after positive transformants were sieved out by pcr, digesiting analysis and sequencing were also used to confirm the positive result more

    所得的dna片段經ecor和not雙酶切後用t _ 4dna連接酶與ppic9k載體進行連接，然後導入大腸桿菌dh5 ，用pcr法篩選陽性轉化子，並用雙酶切和序列測定方法鑒定重組質粒。
  9. Steel frame with welding joint easily occur brittle collapse because of having a low ductility at joints then ， a semi - rigid connected steel frame has large the energy absorption capacity which can resist dynamic loads and the using steel qualities of bracing system and joints are small so adopting a semi - rigid jointed steel frame is economical and stable but for semi - rigid connections are complex and variable ， in conventional analysis and design of steel structures ， it is usually assumed that the connections between columns and beams are either rigid or pinned the analysis of steel frames adopting the assumption can simplify the procedure of analysis and design ， but cannot precisely reflect structural practical circumstance and the errors of calculating results are large ， even ， get incorrect conclusions semi - rigid connection was referred to in chinese code for design of steel structures ( 2001 ， 10 ) ， however ， it isn ’ t specified how to apply semi - rigid joints in design in fact it cannot be carry out the purpose of the paper give a calculating method that accords practical engineering and easily put into effect worthwhile it is going to promote the development of semi - rigid jointed steel frame in design and heighten structural stability in the paper ， at first some commonly employed methods for the modeling of connection behavior are introduced richard abbott function modeling of connection is adopted for extended end plate bolted connection by the 34 test data comparing to regression analysis indicate richard - abbott function modeling of connection represents an excellent fit to test data then after a semi - rigid joint behavior can be modeled as a finite stiffness rotation spring ， base on rotation and displacement equation derive the element stiffness matrixes with semi - rigid connections where the effects ofj ointed flexibility geometric non - linearity and shear forces in the connection deformations have been considered in and fixed - end forces are modified finally, a program for calculating semi - rigid with incremental - iterative method has been

    本文的目的就是為半剛性連接鋼框架的設計提供一種既符合工程實際又簡便易行的計算方法，供規范使用過程的補充、延伸或參考；同時，也將促進半剛性連接鋼框架設計技術的發展，為提高結構安全性能、節省工程成本發揮應有的作用。本文首先介紹了常見的幾種應用較為廣泛的樑柱連接彎矩轉角關系模型，在分析比較的基礎上，選用richard ? abbott函數模型作為外伸端板連接彎矩轉角關系模型，通過對34個外伸端板連接的實驗數據與回歸分析得到的參數比較可知，經回歸分析得到的此模型參數與實驗數據符合較好。然後用彈簧表徵連接點的轉動剛度，根據梁的轉角位移方程推導出半剛性連接的剛度矩陣，在單元剛度矩陣中考慮了節點柔性、幾何非線性和剪切變形的影響，並對固端力進行了修正，最後用增量迭代法編制有限元程序進行計算和分析。
  10. Then a semi - rigid joint behavior can be modeled as a finite stiffness rotation spring. basing on rotation and displacement equation, we obtain the element stiffness matrixes with semi - rigid connections where the effects of material nonlinear, hysteresis behavior and shear forces in the connection deformations have been considered in and at the same time we modify the fixed - end forces. finally, a program for calculating the elastic - plastic earthquake seismic of semi - rigid connected steel frame structure by step and step integration of wilson -

    本文在介紹和分析半剛性連接彎矩與轉角連接關系模型的基礎上，用帶有轉動剛度的彈簧表徵半剛性連接，推導出半剛性連接的剛度矩陣，並對桿件固端力進行修正，同時在考慮剪切變形、材料彈塑性、及構件的動力滯回性能的基礎上，編制了結構彈塑性地震動力時程分析有限元計算程序。
  11. Then after a semi - rigid joint behavior can be modeled as a finite stiffness rotation spring, base on rotation and displacement equation derive the element stiffness matrixes with semi - rigid connections where the effects of jointed flexibility and shear forces in the connection deformations have been considered in and fixed - end forces are modified. finally, a program for calculating system reliability of semi - ridge steel frames with monte carlo method and finite element analysis has compiled for calculating and analyzing. by calculating results of several examples for steel frame in semi - rigid joints case show the change of joint stiffness and other kinds of factors affect the system

    接著本文在介紹和分析半剛性連接彎矩和轉角關系模型的基礎上，用帶有轉動剛度的彈簧表徵半剛性連接，推導出半剛性連接的剛度矩陣，對固端力進行修正，同時考慮了剪切變形的影響，編制了半剛接鋼結構平面框架的有限元程序bhpfl ，並在其基礎上編制了基於蒙特卡洛法的montecarlo - bhpfl有限元相結合的方法，進行體系可靠度的分析。
  12. The conductors placed in the slots of the stator or rotor are interconnected to form windings

    放置在定子或者轉子的槽中的導體相互連接，形成了繞組。
  13. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  14. In order to solve the multi - outgoing campus network ' s routing problem in network address translation ( nat ), a policy routing scheme based on connection tracking information was proposed

    摘要針對網路地址轉換（ nat ）環境下多出口園區網路存在的路由選擇問題，提出了利用連接跟蹤信息來指導路由判斷的方案。
  15. Nowadays the research on the performances of security investment funds in china is mainly concerned with two aspects. the one is the feasibility of the theoretical model of performances and the simple computation by means of indicator formulation, which can be used to compute certain performance indicators, such as average profit rate per week, p coefficient, johnson coefficient, sharp ratio, var, average profit rate per week / var and etc. the other one is the research on the degree to which funds holdings are concentrated and research on the tendency of industry selection in the portfolios of funds managers. every part of market is trying to connect the portfolios selection of funds managers with the mar ket focuses and development directions, hoping that it can lead to the conversion and maturity of the ideas of market investment

    目前，對于中國證券投資基金績效的研究與評估，基本上圍繞著兩個方面，即績效理論模型的可應用性探討或簡單的指標公式套算，計算某些績效指標，如平均周收益率，貝塔系數，詹森系數，特雷諾系數，夏普系數， var和平均周收益率var等幾項指標；和對基金持股集中度的研究，以及由此引伸的對基金經理的投資組合中的行業選擇傾向的研究，市場各方一直在試圖將基金經理的投資組合選擇與市場熱點和發展方向連接起來，並希望籍此引導市場投資理念的轉變與成熟。
  16. Three binary expression vectors were constructed, which harbored the cl - i - 2kl, r - 2kl, and d - l - 2kl - r - 2kl respectively, driven by the camv 35s promoter. then, agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum, w38 ) was carried out. transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis

    通過分子克隆技術，將c1 - i - 2k1 、 r - 2k1和二者的連接（ c1 - i - 2k1 ? r - 2k1 ）分別構建到雙元表達載體上，並使它們分別置於35s啟動子的驅動之下，然後通過農桿菌介導的技術分別對煙草（ nicotianatabacum ， w38 ）進行了轉化，利用組織培養技術再生植株。
  17. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  18. By the same method, the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion. after that, the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method. the pcr amplification indicated the lba4404 strain containning cpti gene. the lba4404 strain was used in genie transformation of mustard

    經酶切和pcr擴增驗證后，以xbai和saci雙酶切pgem ? cp和pbi121 ，將切下的cpti片段和pbi121載體片段連接構建成表達載體pbi121 - cp ，用凍融法導入農桿菌lba4404 ，提取質粒，經pcr擴增檢測，用於芥菜的遺傳轉化。
  19. Armigera was analysed and then registered in genbank and accession number was af515667. the cdna encodes a protein with a plltative 2l amino - acid signal peptide and a 425 amino - acid mature protein with molecular mass of 50 kda

    連接產物轉化jm ，提取質粒進行酶切及pcr分析，將此連接產物命名為pchto將pcht轉化blzi （ de3 ） ，經iptg誘導，提取菌體內容物sds page電泳。
  20. Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169

    將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
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