連續培養 的英文怎麼說

中文拼音 [liánxùpéiyǎng]
連續培養 英文
continous flow culture
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 續 : Ⅰ形容詞（連接不斷） continuous; successive Ⅱ動詞1 （接在原有的后頭） continue; extend; join 2 （...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 連續 : continuation; succession; series; continuity; continuing; running; continuous; successive; contin...
  1. Cercariae were collected, cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum. the schistosomula were cultured in conditional media up to 96 hours. the number of schistosomula was counted and the death rate was calculated

    將條件培養基與童蟲共培養， 96h內連續觀察計數，計算童蟲死亡率，與陰性對照比較，童蟲死亡率最高的基因池進入下一輪篩選。
  2. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  3. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  4. Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna

    中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。
  5. 2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage

    Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。
  6. The chemostat is an important device used for growing micro - organisms in a continuous cultured environment, and a medium of great importance between principles and applications

    利用恆化器連續培養微生物已是微生物學研究中的一項重要的研究手段；是原理和應用之間的一個極其重要的中介。
  7. Potato dextrose agar and grain medium were also used to identify fungi which were not determined by the primary culture. fungi were all secondarily cultured on sabouraud medium to observe the colony ' s texture, colour, growth rate, surface status and reverse pigment. the fungi should be examined by microscope to inspect their microscopic structure from 7th day to 21st day

    使用的培養基有沙氏培養基、土豆培養基、真菌試驗培養基和5種種子培養基，連續培養4周，並隨時觀察菌落的色彩、生長速度、表面狀態、背面顏色等，並從第7天?第21天連續鏡檢以檢查真菌的顯微結構，綜合菌落形態和顯微結構，以確定真菌的種屬。
  8. The time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. for a successful formation of primmorphs, it is mandatory to use healthy sponge tissues and a minimum inoculate cell density is required. culture temperature will need to be adapted to the environmental temperature where the sponges are collected

    對mcp - primmorph的生長，增殖和代謝特徵進行5天連續培養考察，發現海綿細胞團離體培養方法的建立及其成團增殖和代謝規律研究了mcp一prllnmorph培養中存在有絲分裂細胞，但增殖細胞比例僅為巧%左右，且上述培養基對增殖無顯著影響。
  9. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  10. Locations of limit cycles in a nonlinear continuous culture model

    一個非線性連續培養模型極限環的位置
  11. Stability analysis of equilibrium for microorganisms in continuous culture

    微生物連續培養過程平衡點的穩定分析
  12. Study of dynamic behavior in culture of continuous cultivation of microorganisms

    微生物連續培養過程中動態行為研究
  13. The qualitative analysis of a model of predator - prey system with holling ' s functional response

    一類微生物連續培養競爭系統的定性分析
  14. Measuring retention time with static growth curve during continuous culture of high efficient bacteria

    高效菌連續培養中通過靜態生長曲線確定停留時間
  15. In this paper, oscillations for the system of continuous cultivation of microorganism with delay are studied

    本文主要建立了具有時滯的微生物連續培養模型，並進行了分析和計算。
  16. In the first, the significance of the continuous cultivation of microorganism and the problem that will be studied is presented

    第一章緒論，主要介紹了微生物連續培養的意義和本文將要研究的問題。
  17. Methods : isolated rabbit bmsc by adhesion method and cultured it at 37c, 5 % co2 incubator. graphic the growth curve after observed the growth of primary cells and passage cells. cultured bmsc in calcified solution and observe its potential of osteogenic

    方法：貼壁法分離第四軍醫大學碩士學位論文兔骨髓基質幹細胞， 37 ， 5 % co :培養箱培養，觀察原代及傳代細胞的生長發育情況，並繪制生長曲線;在礦化液中連續培養，觀察其骨形成能力。
  18. 3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days, newborn cells derived from regenerative neurospheres were immunostained. 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells, which were randomly divided into one of three groups of 24 wells cells each

    4將傳代培養的神經球離心收集，轉移至24孔培養板，隨機分為3組，每組24孔細胞，置5 % coz 、 370c培養箱，連續培養7d ，觀察神經球的誘導分化。
  19. Applied constant keeper can enable the microorganism to continuously culture in double periods of “ constant flowing method speed ”. ? it has a process as such : according to the multiplying speed that the bacteria cultures in double growing periods, and the speed that it consumes the nutritious material, fresh culturing liquid is continuously added. ? at the same time, the same amount of well - cultured bacteria liquid is continuously drawn out, so that the next two items are kept relatively constant : the added material and the consuming material ; the amount that the bacteria have multiplied and that has been drawn out

    應用恆化器，能使微生物以對數期「恆流法速度」連續培養，即根據菌種在對數生長期繁殖的速度和消耗營養物質的速度，不斷加入新鮮培養液，同時不斷抽出等量培養好的菌液，使加入物與消耗物、細胞繁殖量與細胞物質抽出量，保持相對穩定均恆。
  20. Effect of continuous fertilizing for shajiang black soil on crop yield and soil nutrien content

    砂姜黑土連續培肥對作物產量和土壤養分含量的影響
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