連通因子 的英文怎麼說

中文拼音 [liántōngyīnzi]
連通因子 英文
connectivity factor
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 通 : 通量詞（用於動作）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 連通 : feed through; connection; connected; intercommunication
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. A single - factor experiment was designed to research the effect of nutrition level on body weight of multiparous sows during gestation through investigating the effect of gestation nutrition on the reproduction performance of multiparous sows in subsequent 3 parities and based on regression analysis of feed intake as well as lactation weight loss and reproduction performance during gestation

    摘要採用單因子試驗設計，通過對妊娠期不同營養水平連續3胎經產母豬生產的研究，建立妊娠期採食量與泌乳損失及繁殖性能等之間關系的回歸方程，研究妊娠期不同營養水平對經產母豬體重變化的影響。
  3. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  4. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。
  5. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  6. This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis

    將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。
  7. An idea, simulating the fuzzy model by establishing the fuzzy model and fuzzy control rule of the controlled object is proposed. generally, it is difficult to acquire the accurate mathematical description of the controlled object. a dynamic modulating factor is introduced in the designing of the fuzzy controller so as to improve the precision of the fuzzy control. and a more convenient defuzzify calculation method is adopted. the cart and the single inverted pendulum are taken so as to illustrate the simulation result. in addition, the detailed qualitative analysis of the fuzzy system is achieved. a kind of common method of qualitative analysis is proposed. it can be used to analyse the fuzzy system and describe the behavior of the system state precisely in the phase plane. the simulating results illustrated the method is feasible and available

    提出了在難以精確描述控制對象數學模型的情況下，通過建立控制對象的模糊模型來確定系統的模糊控制規則，並對模糊系統進行模擬分析的思想.利用連續系統定性分析的思想，提出一種對模糊系統進行定性分析的可行方法，在相平面內較為準確地刻畫模糊系統的動態行為.在模糊控制器的設計中，引入動態調節因子來提高模糊控制的精度，並採用了一種便於實際運算操作的解模糊運算方法.在一種簡單的控制對象情況下進行了具體的模擬計算，得到較理想的控制效果.此外對具有精確數學模型的一級倒立擺系統進行此種模糊控制，亦取得很好的模擬結果
  8. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  9. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  10. Secondly, the hyaluronate lyase gene ( hyl ) was cloned into the vector puc19 by pcr using the total ona sample ofs. equi as template and partially sequenced, too

    Equi的總dna為模板，通過pcr方法，克隆了透明質酸分解酶基因（ hyl ） ，測序后連接到表達載體pse380的trc啟動子下游，構建表達質粒pse380 - hyl 。
  11. On the other hand, the research of " forests and water " is from the aspects of the eco - hydrological models of forest ecosystem from, the viewpoint of forest ecosystem and combining with the research on forest structure, function, productivity, energy and material circulation, to explore the laws and internal relation of various forest hydrological phenomenon is the basic topic of research

    另一方面在祁連山水源林生態系統長期定位研究的基礎上，以祁連山青海雲杉為代表運用森林生態學、森林水文學、生態經濟學、生態水文學等學科理論，通過建立單一因子的水文環境因子模型研究森林水文效應；從生態系統觀點出發，結合森林的結構和功能以及生產力和系統能量及物質循環的研究，揭示各種森林水文現象發生和發展的規律及其內在聯系。
  12. Finally, the distribution discipline of the cyclic changing temperature damage factor under the conditions of low temperature through the equivalent assumption and energy equivalent model based on damage mechanics theory. the fatigue life of the 4cm depth sawed cracking road is obtained under the conditions of - 15 based on the shenyang - dalian highway. in this paper, the inhomogeniety of asphalt concrete resulting from temperature change is considered, when the influence of the temperature change on the semi - rigid asphalt pavement concrete is analyzed

    最後，基於損傷力學理論，通過等效應變假設原理和能量等價模型，得到低溫下循環變溫損傷因子的分佈規律，以沈陽?大連高速公路的預鋸縫為工浙江大學博士學位論文廣義荷載作用下道路與軟基共同作用研究王金昌2003年5月程背景，預測了4cm深的鋸縫在一巧路表變溫作用下的疲勞壽命。
  13. Based on the observation data obtained from 69 sampling sites of different age class forests, and by using biomass expansion factor function, the regression equations of stand biomass and volume of the main forest forests in pearl river delta were built, and the regional forest biomass and its dynamics were estimated on the basis of forest inventory data

    摘要利用生物量轉換因子連續函數法，通過69組不同齡級的森林樣地實測數據，擬合了珠江三角洲主要森林類型的生物量和蓄積量之間的回歸方程，並結合3個時段森林清查資料，估算了區域森林生物量及其動態。
  14. At the point that threshold was crossed, the climate driver ? and thus the climate as well ? flipped to a new and different state and usually stayed there for a long time [ see box on next page ]

    一旦越過門檻，氣候驅動因子便連同氣候本身，翻轉至另一全新狀態，而且通常會持續好一陣子（詳見下頁跨越氣候遽變的門檻） 。
  15. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。
  16. Given that the relays are isolated from each other, the terminals of each channel on the switch card are independent from the terminals of the other channels

    由於繼電器是彼此間隔離的，因此開關卡上每個通道的連接端子與其它通道的連接端子是相互獨立的。
  17. By use of the stress free conditions on crack and the continuity conditions of stress and displacement on ideal bonded material interface, the stress field of an bi - material infinite plate with an elliptical inclusion and a deminfinite interface crack are given on the base of the complex potentials solutions obtained above. and the corresponding stress intensity factor k is given

    在該復勢解的基礎上，根據裂紋表面的零應力條件和理想粘接界面上的位移和應力連續條件，通過求解hilbert問題，得到了含有夾雜和半無限界面裂紋的無限大板的應力場，並由此給出了裂尖的應力強度因子k 。
  18. Thus the resistance factor and vibration modes of the soil layer are obtained and used to analysis the pile response. by considering the interaction between the soil layer and the pile with boundary condition of continuity of displacement and equilibrium of force at the interface of soil layer and pile, the dynamic equilibrium equation of pile is solved and an analytical solution for the pile response in frequency domain is yielded, which is used to define complex stiffness and mobility at the level of the pile head. based on the convolution theorem and inverse fourier transform, a semi - analytical solution of velocity response in time - domain subjected to a semi - sine exciting force is given

    首先通過引入勢函數方法對土體位移進行分解，從而將土體動力平衡方程解耦，求解得到了土層的振動模態和阻抗因子，然後利用該解以小應變條件下樁土接觸面上力平衡和位移連續條件來考慮樁土耦合作用，求解樁的動力平衡方程，得到了樁頂的頻域響應解析解、復剛度和速度導納，利用卷積定理和傅里葉逆變換，求得了半正弦脈沖激振力作用下樁頂速度時域響應半解析解。
  19. Combination of this lemma and the hamilton - connectivity involving neighborhood intersection obtains and improves or generalizes a series of classical results involving hamilton - connectivity, at the same time, we construct some extremal graphs to show the improved results to be the best possible ones. as for hamiltonicity, we omit the similar discussion

    由於每一個偶階哈密爾頓連通圖是o -因子-臨界的，我們在最後一章中我們主要涉及哈密爾頓連通性，利用一個重要的引理，我們得到了一些新結果，並且改進了或推廣了一些經典結論。
  20. In doing this, fragmentation is removed, disk space is reclaimed by compacting the pages using the specified or existing fill factor setting, and the index rows are reordered in contiguous pages allocating new pages as needed

    此過程中將刪除碎片，通過使用指定的或現有的填充因子設置壓縮頁來回收磁盤空間，並在連續頁中對索引行重新排序（根據需要分配新頁） 。
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