連鏈位點 的英文怎麼說

中文拼音 [liánliànwèidiǎn]
連鏈位點 英文
linkage site
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 點 : Ⅰ名詞1 （液體的小滴） drop （of liquid） 2 （細小的痕跡） spot; dot; speck 3 （漢字的筆畫「、」）...
  1. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  2. This thesis is a case example the form, first half part introduced the united states california beef the new soldier for characteristics for big king ' s limited company at with ocean fast food in the fierce competition, then by oneself accurate market positioning, integrating our country food profession, practicing specially operating the strategy, making the business enterprise catena the development, most becoming our country fast food industry soldier rising part of textual empress half, from the company gain and loss the factor analysis commence, important function that manage of business enterprise strategy, combined to put great emphasis on to discuss the choice of the market strategied, and related problem, of development etc. of property is in the united states california beef big king ' s limited company, on from now on of the development way of thinking, have to follow of follow the virtuous cycle ' s orbit, and emerge out more the foreground of the organic vitality

    本論文為案例形式，前半部分介紹了美國加州牛肉麵大王有限公司在與洋快餐激烈競爭中，以自己準確的市場定位，整合了我國飲食行業的特點，實踐了獨特的經營策略，使企業連鎖發展，最終成為我國快餐業異軍突起的新軍。本文的後半部分，從公司得失因素分析入手，闡述了企業戰略管理的重要作用，並著重論述了市場戰略的選擇，以及產業鏈開發等相關問題，意在美國加州牛肉麵大王有限公司，在今後的發展思路上，有所遵循的步入良性循環的軌道，展現出更加生機活力的前景。
  3. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  4. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  5. ( 3 ) the researches about the sparse distribution of wavelength converters, the contents include : the heuristic algorithm to decide the number and location of wavelength converters ; the performance comparison of the two different oxcs ( optical cross - connect ) : share - per - link oxc and share - per - node oxc

    （ 3 ）基於稀疏節點波長變換器的研究，具體內容包括： wdm網路中確定波長變換器的位置與數量的啟發式演算法；波長變換器鏈路共享型和波長變換器節點共享型的交叉連接設備的性能研究。
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