達亞克 的英文怎麼說

中文拼音 [dáyǎkè]
達亞克 英文
daillac
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  1. Kerouac does manage now and then to convey a whitmanesque ecstasy.

    凱魯亞克有時確能傳達那種惠特曼式神韻。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  4. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  5. Plant expression vector pespcema was constructed after subcloning in puc121 and other vectors

    經puc121等中間載體的亞克隆，構建了pespcema植物表達載體。
  6. So two widely separated colonies were established in 1607 : one at sagadahoc, near the mouth of the kennebec river, in maine ; the other in modern virginia

    於是兩個相距甚遠的殖民地於1607年建立起來了：一個在靠近緬因州肯納貝克河口的薩加達霍克，另一個位於今天的弗吉尼亞洲。
  7. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  8. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  9. But this delay, as it was foreseen, did not affect phileas fogg s programme ; besides, the mongolia, instead of reaching aden on the morning of the 15th, when she was due, arrived there on the evening of the 14th, a gain of fifteen hours

    但是，這四小時的耽擱，對于福克先生的旅行計劃毫無妨礙，因為這早已在他意料之中。再說，蒙古號本來應在10月15日早晨到達亞丁，而現在才是14號晚上。這就是說，富裕了十五小時。
  10. ( 6 ) pdgf may initiate neuronal differentiation in nsc. nsc induced by pdgf, transcription and translation level of different thrs genes were up - regulated compared with the nsc induced by serum. the results made a great foundation for the further research of the important role of thrs during the cns development, and have much clinical significance on therapy of cretinism ^ subcretinism a

    Thr各亞型在不同分化細胞中表達不一致： （ 0l ；能誘導nsc向少突膠質細胞分化，並通過thr各亞型的時空表達來發揮其重要生理作用門印叩樸能引發vc向神經幾分化，與10血清誘導分化相比能在轉錄與翻譯水平上提高nsc分化后thr各亞型的表達本研究結果將為深入研究t在腦發育中的作用提供寶貴的實驗依據，並對克汀病、亞克汀病以及ms脫髓鞘病等的臨床治療提供新途徑，具有重要指導意義。
  11. Lasorda said chrysler picked chery after looking at potential partners in europe and asia

    萊索達說，克萊斯勒是在研究歐洲和亞洲的潛在合作夥伴后才選定奇瑞的。
  12. Secretary of world packaging organization carl olsmats and president of apf ( asiatic packaging federation ) dharma ratnayake attended the ceremony

    世界包裝組織秘書長卡爾?奧斯邁茨，亞洲包裝聯盟主席達爾馬?拉特納亞克等參加了昨天的奠基儀式。
  13. Robert dallek is a professor of history at boston university and the writer of political biographies. he has also taught at columbia, ucla, and oxford universities

    羅伯特.達萊克，波士頓大學歷史學教授，曾任教於哥倫比亞大學，美國加州大學洛杉磯分校以及牛津大學，著名政治傳記作家，專門研究美國總統歷史。
  14. Famous guest stars on the show have included er s george clooney and noah wyle, jean - claude van damme, chris isaak and julia roberts

    劇中有著名演員喬治.可蘭尼、諾亞.威利、吉恩.克勞德.范-達姆、克里斯.伊莎克和朱莉亞.羅伯茨。
  15. If they win by just a goal, they also have to hope werder bremen do not win at olympiacos cfp in the other group c game

    如果他們只贏一個球，他們也得寄希望于雲達不萊梅在c組另一場比賽中對陣奧林匹亞克斯不勝。
  16. Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed

    菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。
  17. The two genes were subcloned into pet30 vector, and recombinant proteins with predicted molecular weight were achieved. in this paper, we constructed the recombinant e. coli highly expressing pil - 10 in order to study the pleiotropic immunological regulations in various diseases

    然後將p35基因和p40基因分別亞克隆到原核表達載體pet30c 、 pet30b中，用iptg誘導培養，分別表達出了預期分子量大小的重組蛋白。
  18. Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ), then subcloned into the eucaryote expression vector pcdna3. 1 ( + ) after sequence analysis

    將halr基因克隆到原核融合表達載體pet28a （ + ）上，序列分析后亞克隆到真核表達載體pcdan3 . 1 （ + ）上。
  19. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  20. The vp6 gene was subcloned from recombinant plasmid pmd18 - t - vp6 into expression plasmid pet - 30a. the recombinant plasmid pet - 30a - vp6 was transformed into e. coli bl21 ( de3 ) and induced with iptg. not only a fusion protein about 45ku as we expected was found but also several smaller polypeptides were observed

    從重組質粒pmd18 - t ? vp6上將vp6基因亞克隆到表達載體質粒pet - 30a ，轉化大腸桿菌bl21 （ de _ 3 ） ， iptg誘導以後表達出預計的45ku的融合蛋白，同時還有一些小的蛋白表達出來，光密度掃描對表達產物進行初步定量表明， 45ku融合蛋白占菌體總蛋白的26 . 5 。
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