達克隆 的英文怎麼說
中文拼音 [dákèlōng]
達克隆
英文
daclon-
Cloning and expression of a new acyl - homoserine lactone hydrolase gene
一種新的乙酰高絲氨酸內酯水解酶基因的克隆和表達Canine adenovirus type 2, cav
表達盒克隆入犬2型腺病毒Stable suppression of afp gel expression by rnai in smmc - 7721 cells
表達質粒穩定轉染肝癌細胞克隆的構建A kind of new - style portable zxc air pump
基因的克隆與表達分析The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Cloning and expression of antibacterial peptides pexiganan and ib
的基因克隆及表達Dacron polyester fibers
達克隆聚脂纖維Use 3 - 5 adjectives to describe aladar ， kron ， neera and bruton. then tell the reason
用3 - 5個形容詞來描述一下艾力達、克隆、妮娜和布魯頓，然後說明理由。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。Of the differentially expressed clones, 104 clones were highly expressed in the ovary at stage iii, the remaining clones were highly expressed in the ovary at stage ii
結果共獲得2倍以上差異表達克隆167個。其中期高表達克隆104個，期高表達克隆63個。Conclusion constructed the high - level expression clone of echistatin in e. coli. the expression of recombinant protein is higher, it make the further study of echistatin feasible
結論成功構建了echistatin的原核高效表達克隆，表達量高於現有國內外研究水平，為echistatin功能及相關疾病的研究奠定了基礎。Chapter 3 sequencing cdna fragments of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) partial clones were sequenced which were selected from the differentially expressed clones screened by cdna macroarray analysis
3中華絨螯蟹卵巢發育相關基因cdna片段的序列測定從cdna微陣列篩選的差異表達克隆中選取部分克隆進行測序。Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %
結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。Differential hybridization of human testis cdna micorarrays human testis cdna microarrays were constructed by spotted the pcr product amplified from human testis library. then membranes were hybridized using the probes of embryonic testis ( 6 months ) and adult testis
人睪丸cdna微陣列雜交從人睪丸文庫中pcr擴增插入片段，將pcr產物點膜制備成cdna微陣列，然後提取胚胎和成人睪丸的mrna ，制備成探針，分別與微陣列進行雜交，獲取差異表達克隆。In the first part, the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative, to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above
方法： （ ）以融合蛋白prpap通過瞬時表達克隆法篩選兩個cdna文庫，或者通過與胚胎腦的結合實驗篩選有豐富結合蛋白的腦區，以圖構建cdna文庫並進行表達克隆的篩選。After sds - page and densitometric scan analysis, the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 %. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %
今士考個二目卜乙成功構建了ptxbi一hng及ptxbi一m一insulin原核表達克隆，並獲得了高效表達，經過純化得到純度人於80 %的融合蛋白，並對人胰島素突變體融合蛋白進行了初步活性測定。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody. our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4. after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4. our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我們構建的rhpn原核高效表達系統經m page及凝膠密度掃描分析結果表明， rhpf4表達量占菌體總蛋白量的25 30 ，較原表達克隆pt7 7 rhpf4提高了近80倍，經快速高效的包涵體分高純化工藝和復性工藝， rhpf4具有野生蛋白活性。The present investigation is favorable for screening the new generation of anti - tumor peptides. [ objective ] ( 1 ) to clone and express fasl - ecd in e. coli
[目的] （ 1 ）分析人黑色素瘤細胞（ a375 ）中fasl基因的表達，克隆人fasl胞外區的編碼基因，並在大腸桿菌bl21中進行表達。Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。分享友人