適性酶 的英文怎麼說
中文拼音 [shìxìng]
適性酶
英文
adaptative enzyme-
It was very slow at 5 but become higher above 25. the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin. through the gel filtration chromatography and ion exchange chromatogra
羔羊胃蛋白酶最適凝乳溫度為45 』 c ；在45處理30inin ，酶活性開始下降， 60c處理30lliln ，酶活性完全喪失；酶的最適ph為1It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition
對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。The results were as follow : in the process of its male organ development, these are many abnormalities, such as : premature tapetal degradation, giant tapetal cells, the great vocalization of the tapetal cells and inadaptable function of callose enzyme, which lead abnormal and premature degradation microspores and male gametophytes
實驗結果表明： 1蝟實的雄性生殖器官在發育過程中，出現絨氈層細胞的液泡化、肥大、提前降解及不適時釋放胼胝質酶等異常現象，造成蝟實花粉發育過程中出現畸形、解體。Second, the activities of protective enzymes i. e. superoxide dismutase ( sod ), peroxidase ( pod ), and ascrobate peroxidase ( apx ) exhibited down - up - down trends when treated with 2 @ and 8 @, and these activities sustained at a relatively high level. this indicated euonymus japonicus " zhuzi " had relatively high adaptability to cold stress
（ 2 ） 『粗枝』大葉黃楊在零上低溫時，細胞保護酶超氧化物歧化酶（ sod ） 、過氧化物酶（ pod ） 、抗壞血酸過氧化物酶（ apx ）的活性先降低後上升，又下降並維持較高的水平，表明『粗枝』大葉黃楊對低溫脅迫有一定的適應性。It has been 40 years since dobereiner and ruschel isolated the nitrogen - fixing bacteria from the rhizosphere of sugarcance plants and demonstrated the potential of diazotrophs to associate with graminaceous plants. more recent evidence of significant biological nitrogen fixation in economical important graminous species, particularly sugar cane, rice and forage grasses, has induced tremendous interest in ni fixation by non - legumes
本研究分離、篩選得到一株固氮酶活性高且穩定，生長勢強的聯合固氮菌株，並對其分類地位、形態及生理特徵、對環境的適應性及其對植物的促生效果和作用機理作了系統研究，得結果如下。The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label
酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。Adaptive cytoprotection of pepsin and its possible mechanism
胃蛋白酶的適應性細胞保護作用及其機制The effort of manganese removal was studied and the kinetics of manganese removal was tried to establish. the factors of dissolved oxygen concentration, fe2 + concentration, ph, p concentration and closing of the filter were studied to evaluate their effort for biological manganese removal, and the correlation of residual manganese and oxidation - reduction potential was also discussed. as the iron content of water was high, experiment results showed that the reaction was zero order, as the iron content of water was low, the reaction was first order. the time needed for the cultivation of biological manganese removal was 60 70 days. the filter operated at the filtration rate of 8 10m / h, silica sand of effective size 0. 95 1. 25mm filled the filter to a depth of 1200mm
試驗結果表明，成熟后濾砂表面濾膜的x射線衍射圖譜與mno _ x ? 5h _ 2o （ x = 1 . 86 ）的x射線衍射圖譜一樣，濾膜成熟后的結構在進水物質不發生變化的情況下不發生變化;合適的碳磷比對生物除錳有明顯的促進作用，試驗條件下的投磷量不會對出水造成二次污染;生物除錳需要亞鐵的參與，亞鐵的存在除了能夠促進微生物分泌胞外酶並刺激其活性外，還通過鐵離子的變價傳遞電子，催化錳離子的氧化反應，從而促進對二價錳的降解。( 3 ) uv - b radiation increased the phenylalanina ammonia - lyase ( pal ) activity in leafy thallus of duckweeds, and as a consequence, flavonoids were accumulated. flavonoids synthesis induced by supplementary uv - b is an adaptive response of duckweeks under enhanced uv - b radition
（ 3 ）紫萍可以通過提高uv - b吸收物質如類黃酮合成酶苯丙氨酸氨裂解酶（ pal ）活性，促進類黃酮的積累來適應uv - b輻射的脅迫。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time
石蠟切片進行原位pcr檢，預處理分別滴加loom歲血的蛋白酶k20閃消化，以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準，確定最適消化時間『 ， ，研究不同固定時間與蛋白酶消化的相互影響和關系，同時檢測石蠟切片的mn基因型。The purified enzyme was a monomeric protein whose optimum ph was 8. 0. inhibitor study demonstrated that it was a metalloprotease and contained sulfhydryl groups essential for catalytic activity. its activity was inhibited by divalent cations such as zn2 +, mn2 +, and the kj values of zn2 +, mn2 + were imm
這種酶溫度適應范圍較廣，在較大溫度變化區間內都具有明顯的活性，但是對緩沖溶液ph值的變化卻很敏感，體外以明膠為底物時最適溫度為38oc ，最適ph值為8It is a combination high quality natural or synthesized fabrication like linen, tencel, elastic fibers, cotton and compound processes like knitting, weaving, jacquarding. the fabric is treated with green high tech processes as biological enzyme softening and non - formaldehyde crinkle killing. the product is featuring natural health - care function, body suitability as well as graceful and fashionable design
採用羅布麻tencel彈力纖維和高支棉等多種優質天然合成保健功能纖維，並應用梭織針織提印花結合等多種復合生產工藝和生物酶柔軟無甲醛抗皺等綠色高新整理技術，具有多種顯著的天然衛生保健功能和良好的人體形態舒適性，而且具有新穎別致和高檔華貴的特殊品質外觀Then the nucleuses were splitted by sds, dna was precipitated by iso - propanol or alcohol. the average dna yields were 527 u g / g with fresh base
基因組dna平均得率為527 g g鮮重，經鑒定適合進行限制性酶切和rapd擴增。Pcr products were inserted into pbluescriptsk using psti and xhoi. the element bearing resistance to streptomycin ( sm ) and spectinomycin ( sp ) was excised from php45 and inserted into gene coding region. the disrupted gene was isolated as a pstl and xhol fragment and cloned into prl271
首先將pcr擴增片段（ 1 . 5kb ）利用pst和xho插入常規克隆載體pbluescriptsk ，利用基因內部合適的酶切位點插入sp sm抗性片段，再利用pst和xho位點將已插入抗性基因的pcr片段，轉入整合載體prl271中獲得體外基因中斷，最後利用三親本雜交獲得體內突變。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。But the results of restriction enzyme reaction are not satisfied. there may be two reasons which lead to these results : one is that the restriction enzymes are not suitable for this plasmid, that is to say there are no appropriate cut sites on the plasmid for these restriction enzymes ; and the other is that the purification of the plasmid is not enough for restriction enzyme reaction, and some impurity affects the last results
為了建立質粒的物理圖譜，我們對所提取的質粒進行了限制性酶切消化，摘要但是未能取得滿意的結果，原因可能有兩個：一，所選擇的酶不合適，在質粒上沒有合適的酶切位點；二，質粒的純度不夠，影響了酶切反應的結果。分享友人