選擇培養基 的英文怎麼說
中文拼音 [xuǎnzháipéiyǎngjī]
選擇培養基
英文
selective culture-medium- 選 : Ⅰ動詞1. (挑選) select; choose; pick 2. (選舉) elect Ⅱ名詞(挑選出來編在一起的作品) selections; anthology
- 擇 : 擇動詞(挑選) select; pick; choose
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 選擇 : select; choose; opt; election; choice; culling; alternative
-
Most grow best and form pycnidia earlier on ma, and pda is slightly not fitful for pycnidia growth of the kind of fungi. none of the species can produce ascocarps in 60 days. esterase isoenzymes of 12 strains of rhytismataceae were studied by polyacrylamide gel electrophoresis and clustering analysis of upgma and 12 strains were classified into 4 groups when correlation coefficient is 3. 1
結果表明大多數種在ma平板上菌落營養生長最好,產生分生抱子器及分生抱子的時間較早、能力較強; oa也是可選擇的培養基之一:而p a較不適宜於該類菌物的營養生長和分生抱子器產生。Pig blie salts is the sodium salt of a conjugated bile acid made from pig bile, often used in bacteriological culture media as a selective inhibitory agent
性狀:本品系由豬膽汁精製而成的一種膽汁酸鈉鹽,是微生物培養基中常用的選擇性抑制劑。The lipase b from candida antarctica ( cal - b ) displays high enantioselectivity on a broad range of substrates, making it an accepted biocatalyst for asymmetric organic chemistry
摘要從南極假絲酵母中提取的脂肪酶b ,具有較高的對應異構體選擇性,廣泛應用在培養基中,作為不對稱有機化學的生物催化劑。The results indicated that : jaj could selectively stimulate the reprduction of bifidobacteria in vivo and inhibit the growth of e. coli which is a main parasitic basterium in human intestinal tract ; moreover, jaj could apprarently improve intestinal tract function. in tested group, the mice excreted smoothly and the faecal particles of mice were big and wet, but in control group, the faecal particles of mice were small and dry. lt was suggested that inulin may be the important effective component in jaj which promoted the reprduction of bifidobacteria in vivo. at last, the effects of ja on the bile salt resis tance of bifidobacteria were studied. the test proved that : deoxycholic acid na - salt ( dca - na ) had intensely toxical action on blm and bbm ; adding glucose and fructose in media could decrease the lexical action on bbm. but inulin and jap had not apparent effect
在通過單菌株檢驗和混菌檢驗確立了一種選擇性雙歧桿菌培養基之後,進一步以健康昆明系小鼠為實驗動物,研究了菊芋在動物腸道內對雙歧桿菌的影響,動物實驗結果表明,菊芋汁在體內對雙歧桿菌有選擇性促進生長作用,而腸道中主要條件致病菌?大腸桿菌的生長受到抑制;菊芋中的菊糖成分可能對菊芋在體內選擇性地促進雙歧桿菌生長起了主要作用;此外,菊芋還具有明顯的整腸作用,同對照組相比,飼喂菊芋汁的小鼠排便順利,糞便顆粒大且濕潤。As a selectable agent, concentration of kanamycin is different with medium of buds induction. concentration of kanamycin is higher in the medium that has higher frequency of buds induction
在不同的出芽培養基上,卡那黴素的選擇濃度也不同,出芽率越高,卡那黴素所需的濃度越高。Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘,然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株;接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增,結果表明其中7個菌株具有nifh基因,這7個菌株的編號依次為: c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。Edible gelatine. detection of coliforms. 30 degrees c culture method on liquid selective medium
食用明膠.大腸桿菌的檢測.在30時液體選擇培養基的培養法Edible gelatine. determination of fecal coliforms. 44, 5 degrees c culture method on liquid selective medium
食用明膠.糞便中大腸桿菌的檢測.在44 . 5時液體選擇培養基的培養法A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate
以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫,在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ,插入片段分別為7kb和11kb 。The results show that most isolates produce enough condia. which are similar to those directly received from the hosts, when they grow on the twa + w culture media for about 1 - 2 weeks, under 18 - 22 and alternative nuv light for 12h and in dark for 12h. but we should try several different kinds of culture media to produce the natural condia for some special species
總結如下: ( 1 )適宜培養基及適宜培養條件:使用自來水洋菜麥稈培養基( twa + w ) ,近紫外燈照射下, 18 22下, 12h光照與12h黑暗交替培養時,大部分菌落生長較好;但對個別的種也應使用多種培養基,選擇最接近自然狀態的培養基和培養條件。A few mutants were found on the plate containing nitrobenzene selective medium. their colony appearances have some difference from the wild strain. some evidences show that their growth characteristics on the selective medium are related to the plasmids harbored in their cells
對菌株的自發突變株的研究表明,這些菌株在硝基苯選擇培養基上的生長特性是與它們細胞中所含質粒的特性相關的。To determine the plasmid function, the experiment is done to cure the plasmid from the cell, which will cause some change on the strain physiology. the result show that after curing the plasmid, the strain cell cannot grow on the selective medium containing nitrobenzene
為了考察質粒的功能,進行了質粒消除實驗,結果表明,質粒消除后的菌株細胞難以在硝基苯選擇培養基上生長,表明這個質粒是與硝基苯的降解代謝過程有關的。A total of 168 strains of actinomycetes, of which 23, or 13. 7 %, displaying antitumor activity, was isolated from near - shore marine samples collected at wenchang mangrove, danzhou harbor and yanpu harbor, three isolation methods were employed, which are sds pretreatment, phenol pretreatment, heating pretreatment and potassium dichronate selection culture, and various media were used
先後從文昌紅樹林、儋州港以及洋浦港採集樣品,採用苯酚、 sds 、加熱三種樣品預處理法和添加重鉻酸鉀的選擇培養法,並選用多種培養基對這些樣品進行了具有抗腫瘤活性海洋放線菌的分離篩選。共分離到168株放線菌,其中23株具有較強的抗腫瘤活性。To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 ( b ) ,根據已構建好的含有mbl野生型基因的t載體pgem - mbl ,設計一對引物, pcr擴增mbl基因,凝膠回收,雙酶切pcr產物和pet28 ( b )質粒, t4連接酶連接,轉化大腸桿菌dhsa ,氨芋選擇培養挑取克隆鑒定。Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained
方法:基因工程p24抗原免疫小鼠4次,歷時2個月,取脾細胞與骨髓瘤細胞株sp2 0 ,用peg融合, hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞,三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。After 4 times selected culture by ppt, 347 resistant calli were obtained. the ppt resistant calli were transferred to ms medium with 2. 0 mg / l 6 - ba, 0. 1 mg / l naa for regeneration
0mg l +蔗糖30g l的選擇培養基中進行選擇培養,每10天繼代1次,連續選擇4次,共得到347塊抗性愈傷組織。To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象,選用真核表達質粒pci - neo ,根據已構建好的含54位密碼子突變型mbl基因t載體的結構,設計合成新的引物, pcr擴增54位密碼突變型mbl基因,凝膠回收,雙酶切pcr產物和pci - neo質粒, t4連接酶連接,將前者克隆至後者的sma和sal位點,轉化大腸桿菌xl - 1blue ,氨芐選擇培養。Putatively transformed shoots all attained from torenia fournieri lind
分別得到了在選擇培養基上生長的抗性植株。4. transformation and selection of plants. leaf explants had been pre - cultured for 2 days, then immersed in agroba - - cterium suspension for 5 ~ 6minutes
轉化體篩選及植株再生將預培養2天的外植體與農桿菌菌液浸染5 6分鐘后,共培養2 3天,然後轉化到含km75m旮的選擇培養基上進行分化篩選,再轉入k 。A mutant probably harboring more plasmids can grow better on nitrobenzene selective medium than other strains. the plasmid harbored in this mutant is also a few kb smaller than others. so a probability is supposed that a certain plasmid dna fragment deletion made the number of plasmid copy change, which affected the mutant growth on the selective medium
一株質粒含量較高的突變株在硝基苯選擇培養基中的生長情況要好於其它菌株,其所含的質粒也略小一些,因此我們推測,某個質粒片段的缺失造成了質粒拷貝數的變化,從而影響了它的生長特性。分享友人