選株 的英文怎麼說
中文拼音 [xuǎnzhū]
選株
英文
roguing-
High frequency of bud induction was found on ms medium supplemented with 6 - ba the optimum medium for adventitious bud induction was ms + l. 0mg / 1 naa + 0. 5mg / l 6 - ba, and ms + 1. 5mg / l naa + 0. 5mg / l iaa for bud rooting
門)誘導毛狀根體細胞胚再生植株。通過正交實驗篩選出了毛狀根體細胞胚誘導的最佳培養基為: ms 05mg lnaa 0 sing kt 30gil蔗糖。Agronomic testing of the selections begins in line tests established from the seedling nurseries, usually single row 3-6 ft long.
選系的農藝測定從在苗圃建立的品系測驗開始,一般單株得長36英尺。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Screening of hypo virulence of colletotrichum gloeosporioides and its control effect on apple anthracnose
蘋果炭疽菌低毒性菌株的篩選及控病效果The strain 2009 and the pure culture were used as experimental materials to study the relationship between the pregermination methods of auricularia auricula and the emergence rate, the contamination rate and the yield by six methods
摘要以黑木耳菌株2009作試驗菌株,選用木屑培養基作試臉材料,在同一黑木耳栽培菌培養室採用6種不同的催芽方法,研究了催芽處理方法與耳基分化率、污染率和產量之間的關系。Wild arabidopsis thaliana seeds were mutagenized with y - ray, and m2 populations were screened in the medium containing 200mmol / l nacl. 352 potential high - salt - tolerance mutants were selected from 80, 000 m2 seedlings, and 4 of this m2 mutants showed high - salt - tolerance in their m3 generation. this high - salt - tolerance mutants were backcrossed with the wild type arabidopsis thaliana
在含200mmol lnacl的改良ms培養基上,通過對約80 , 000株m _ 2代幼苗的篩選,共獲得352株根仍表現向地性生長或真葉不變為褐色的植株作為可能的突變體。In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail
本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌,並對其抗菌活性物質細菌素進行分析,為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302
選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜,並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 : 88 : 22等培養再生植株進行基因表達的微陣列檢測,結果表明: ( 1 )三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。Construction of bivalent vaccine candidate expressing hspa and ureb subunit from helicobacter pylori
雙價侯選疫苗株的構建One cadmium resistance strain of fungi designated as f2 which could survive 2000mg / l of cadmium was isolated from blende soil with high content of cadmium. it showed high ability of cadmium accumulation. it belongs to phoma sp
從高鎘含量的閃鋅礦土樣中經分離、篩選,得到一株可在2000mg l鎘濃度下存活並對鎘具有較強的富集能力的絲狀真菌,編號為f2 ,經形態學鑒定歸入莖點霉屬( phoma ) 。The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes
試驗表明,在農桿菌介導高羊茅遺傳轉化中,抑菌劑宜選用梭節青霉素;以潮黴素作為選擇劑時,採用低濃度( 50一15om留l )連續篩選的方式比較合適,在該方式下,獲得的轉基因植株較多。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物,以增殖的兩株iltv的dna為模板,分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上,轉化感受態大腸桿菌,通過篩選對iltvtk基因的陽性克隆進行擴增培養。Through a series of mutagen tests, a mutant strain of monascus spp ( m 12 - 69 ) from ml2 was acquired, which can produce much higher monacolin k than m12, and almost same citrinin level as m12
以之為出發菌株,通過誘變篩選,獲得一株monacolink產率有明顯提高而桔黴素產率僅為0 . 13ng g ( elisa )的突變株,編號為m12 - 69 。Two strains of lab, one coccus and one bacillus which are the normal residents in intestinal tract and which could be the host bacteria, were respectively isolated from lab sanitarian products
從乳酸菌保健晶中分離篩選到兩株可以作為受體菌株的乳酸菌,一株球菌和一株桿菌。Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.
關于花色結構基因查爾酮異構酶chi ( chalconeisomerase )基因對花色改變的直接報道很少,因此,本論文選用了chi基因為目的基因,以純深紅色和純白色矮牽牛( petuniahybidavilm . )為材料,研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用,並在花色改變植株中首次觀察到花器官變異。Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied
植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定,通過初篩和復篩,得到一株產植酸酶較高的黑麴黴( aspergillusniger ) an00101菌株。In this paper, a strain was selected with high esterase activity. its characteristics of production and enzymology and separation of l - lactic acid were investigated. through primary screening and rescreening, a fungus, f - 16, with high esterase activity was selected
本文選育出了高活性酯酶產生菌株並對該菌體的產酶性質、酶學特性及酶對乳酸乙酯的手性拆分后產物分離作了一定的研究。Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa. applying the x2 test, optimal concentration of antigen was determined, and the optimum dilution of enzyme hrp - spa was 1 : 10. the high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis, pasteurellosis, chlamydiosis, hcv, ppv, brucellosis, erysipelas suis, colibacillosis, prv
試驗中對多種方法提取的診斷抗原進行了比較、篩選;確定了dot - ppa - elisa的最佳工作條件;測定了st - 171株的免疫豬血清抗體及c群、 2型人工感染豬血清抗體效價,並初步確定了該方法的陽性標準。This has allowed the development of a balanced - lethal system for the expression of heterologous antigens in vaccine strains using vectors containing the wild - type asd gene from s. typhimurium and asd mutant salmonella hosts
Sds一page和westem一blot檢測表明,菌苗侯選株能夠分別表達出相對分子質量為64kda的尿素酶b蛋白與77kda的尿素酶b和熱休克蛋白a的融合蛋白。分享友人