酵母目 的英文怎麼說

中文拼音 [jiàomǔmù]
酵母目 英文
saccharomycetes
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 目 : Ⅰ名詞1 （眼睛） eye 2 （大項中再分的小項） item 3 [生物學] （把同一綱的生物按彼此相似的特徵分為幾...
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  1. Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome

    為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。
  2. At present, most of lactases used in industry production come from kluyveromyces, aspergillus niger and aspergillus oryzae

    目前，工業生產中使用的乳糖酶主要來源於乳酸克魯維斯酵母菌、黑麴黴和米麴黴。
  3. Now our main products include ; calcium propionate, sodium propionate, sodiumacetate, animal and vegetable hydrolyzed protein. yeast extracts. activated carbon for protein. etc

    目前我所生產的產品有：丙酸鈣、丙酸鈉、乙酸鈉、動植物水解蛋白、酵母抽提物，蛋白專用活性炭等。
  4. Research into potentially protective roles for magnesium found in legumes, nuts, seeds and green leafy vegetables ; chromium found in liver, brewers yeast, wholegrains, nuts and cheeses ; and vitamin e found in many foods but especially rich sources include fortified margarine, vegetable oils, soybeans and some nuts is still ongoing

    關于鎂存在於豆類堅果種籽和綠葉蔬菜中鉻存在於肝臟啤酒酵母全穀食品堅果和乳酪中和維生素e存在於許多食物中，在強化黃油植物油大豆和某些堅果中尤為豐富對糖尿病的潛在預防作用，目前仍處于研究之中。
  5. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  6. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  7. Shows how fibroblasts from human dermis are isolated by using enzymes and then cultured in nutrient medium

    示?如何使用酵素分離人類皮膚的纖維組織母細胞在營養培養基培養，如何計算數目及液體氮冷凍儲存法。
  8. Objective : to investigate pathogenic bacterium and drug resistance of the vulvovaginal candidiasis ( vvc )

    摘要目的：了解外陰陰道假絲酵母菌病的致病菌種及耐藥情況。
  9. Protein and nucleic acid in yeast cells were degraded, then the product was refined into nutritional seasoning, which was in step with current tide of nutrition and nature

    摘要酵母抽提物亦稱酵母味素，是將酵母細胞內蛋白質、核酸等進行生物降解，經加工而成的營養型天然調味料，符合目前「天然、營養、回歸自然」之潮流。
  10. This paper summarizes the commonly used methods for the research of gene function of filamentous fungi, such as transposon tagging, gene knockout, rna interference, over - expression and yeast hybrid system, and provides a discussion on the advantages and disadvantages of those methods

    本文總結了目前絲狀真菌基因功能研究中常用的方法，如轉座子標簽法、基因敲除技術、 rna干擾、超表達及酵母雜交系統等，並對各方法在絲狀真菌研究中的優缺點進行了闡述。
  11. Ozone can oxidize ethane and kills fungus and yeast. in addition, ozone can prevent fruits from metabolism and slow down the life process

    臭氧可氧化乙烯，殺死黴菌與酵母菌；另外，臭氧可抑制果蔬的新陳代謝，減緩它的生理老化過程，從而達到保鮮的目的。
  12. The expression experiment is carried out with the methods introduced by expression guideline. according to the result of sds - page, the target protein is expressed successfully in the pichia

    按表達手冊進行初步表達實驗，經sds - page檢測表明，酵母重組子分泌出了目的蛋白。
  13. But gene targeting was mainly applied to such field as bacterium, yeast and some epiphyte at present, seldom used in higher plants because of the lower frequency of homologous recombination

    但基因打靶技術目前主要應用於細菌，酵母和一些絲狀真菌，在高等植物中應用極少。原因是高等真核生物中同源重組的發生頻率極低。
  14. Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide, which was identified by sds - page and western blotting

    培養多拷貝插入的酵母細胞，用甲醇誘導目的蛋白的分泌， sds - page和westernblotting鑒定表達蛋白。
  15. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  16. Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169

    將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
  17. The positive transformants with the integrates mn - sod gene and cuzn - sod gene were identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein and cuzn - sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native - page and enzymatic activity test

    Pcr鑒定進一步說明，目標基因已經重組到宿主基因組染色體上； 0 . 5甲醇誘導表達后，活性電泳出現明顯活性條帶，重組酵母發酵液中mn - sod的活性約是對照菌株sod活性的2 . 6倍； cuzn - sod酶活力約是對照菌株sod活性的1 . 3倍。
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