酵母菌素 的英文怎麼說

中文拼音 [jiàomǔjūnsù]
酵母菌素 英文
saccharomycetin
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 酵母菌 : saccharomycetes
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  1. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  2. Conclusions : monilia albicans bacteria is the most important source of vvc infection, itraconazole and fluconazol were the most resistant drug for candida mycoderma bacteria, but amphotericin and 5 - flucytosine were the most sensitive

    結論：外陰陰道假絲酵母菌病主要由白假絲酵母菌感染所致，假絲酵母菌對伊曲康唑、氟康唑耐藥率最高，對兩性黴素及5 -氟胞嘧啶最敏感。
  3. There is statistical significance between the control group and the observation group ^ 2 = 137. 18, ( p < 0. 01 ) drug resistance rate from high to low of the frequently used antimycotic drug for candida mycnderma bacteria are itraconazole, fluconazol, econazole, amphotericin in turns

    常用抗真菌藥物對假絲酵母菌耐藥率由高到低依次是伊曲康哇、氟康唑、益康唑，最低為兩性黴素。
  4. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  5. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  6. Although yeast cannot digest cellulose or lignin, the molecules that form a plant ' s skeleton, some bacteria and other species of fungi are able to do the job

    雖然那些組成植物莖干纖維素和木質素的分子不能為酵母所消化，但是某些細菌和其他種類的真菌可以完成這項任務。
  7. Effect of yeast cultures on fibrolytic bacterial population and activities of fiber hydrolytic enzymes in the rumen

    添加不同酵母培養物對瘤胃纖維分解菌群和纖維素酶活的影響
  8. In the bioassay using saccharomyces cerevisiae as indicator, bl10 and bl19 were found unable to produce antibiotic fr - 008 while the other four had no detectable changes in productivity. obviously, the gene replacements happened in the 5. 2kb + 7

    在以啤酒酵母為指示菌的生物測定中，未觀察到基因中斷菌株bl14 、 bl15 、 bl16和bl17的產素表型的變化，而bl10 、 bl19以及所有的雙交換菌株都喪失了產生fr - 008的能力。
  9. The elements affecting the formation of high alcohols include raw material, composition of malt liquid, sorts and quantity of yeast, fermentation condition, concentration of oxygen dissolution, fermentation degree and storage period

    影響高級醇形成的主要因素包括原料、麥汁的組成、酵母菌種類和接種量、發酵條件、溶解氧濃度、發酵度和貯存期。
  10. Common signs of allergies are itchy skin, red itchy paws, chewing paws, yeast infections, ear infections, and skin infections that may respond to antibiotics but reappear as soon as the antibiotics are discontinued

    常見的過敏癥狀有皮膚瘙癢、爪子紅癢、啃爪子、酵母菌感染、耳部感染，以及可以由抗生素治愈，但一經停用抗生素又復發的皮膚感染等。
  11. Introduced beer brewing in the process the diacetyl formationmech anism and the influence factor and the control method, from theyeast mold mushroom spawn, the wheat juice component, pass craft and so on oxygen quantity carries on the adjustment, may in the ac tivecontrol beer the diacetyl content, the improvement beer maturity

    摘要介紹了啤酒釀造過程中雙乙酰的形成機理及影響因素和控制方法，從酵母菌種、麥汁組分、通氧量等工藝進行調節，可以有效控制啤酒中雙乙酰的含量，改善啤酒的成熟度。
  12. A study of yeasts during the delignification and fungal transformation of wood into cattle feed in chilean rain forest

    中譯：酵母在智利原生平原雨林牛隻飼料中進行木質的去木質素作用及真菌類轉換等的相關研究。
  13. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  14. Then, tlc was used to purify them, and antibiotic experiments were made to define which was the main antibiotic substance. results showed, orange pigment was the major antibiotic substance, and it could inhibit bacteria, but had no effect on yeasts and algae. the diameter of the inhibition zone was directly proportional to the value of absorption of orange pigment

    （ 2 ）利用吸附柱分離三種色素，並用tlc法純化，刮取相應的色素點， 70乙醇溶解，濃縮，進行抑菌實驗，證實橙色素是主要的抑菌物質，對細菌具有較強的抑制效果，其抑菌性與其吸光度呈正比變化，橙色素對酵母菌和黴菌無抑制效果。
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