酵素分析 的英文怎麼說

中文拼音 [jiàosùfēnxī]
酵素分析 英文
enzymatic analysis
  • 酵 : 動詞（發酵） ferment; leaven
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 酵素 : biocatalyst
  1. In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail

    本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌，並對其抗菌活性物質細菌素進行分析，為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。
  2. Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

    採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。
  3. The kinetics of erythromycin fermentationin an air - lift bioreactor

    氣升式生物反應器內紅黴素發酵的動力學分析
  4. Figure 2 : sds - page analysis of purified glucose oxidase

    圖2 :利用sds - page分析純化后的葡萄糖氧化酵素。
  5. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  6. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  7. The combined sampling pcr detection method was found to be fully feasible for the rapid ( approximately 2. 5 h ) and highly specific ( no cross - reactivity ) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms

    使用即時定量聚合酵素反應技術結合個人采樣器採集空氣中病毒，證明於空氣中含有其他微生物之情形下本技術對偵測特定病毒之所需分析時間相當短且特異性相當高。
  8. The cytogenetic tests deal with all chromosomal analysis for different diseases. the molecular tests investigate about 40 genetic diseases and the biochemical tests mainly investigate enzymes and proteins products of genetic diseases

    細胞染色體分析對不同疾病進行染色體分析，分子遺傳學檢驗對大約40種的遺傳疾病進行測試，而生化遺傳檢驗則主要檢驗病人體內酵素及蛋白質的含量，以測試有關的遺傳疾病。
  9. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  10. Results according to the analysis and determination of imperatorin, carbohydrate components, amino - acid, extracts, ash and water content, the results showed that the harvest period of radix giehniae in october was appropriate, the process of being sliced with skin and dried under the sunshine or in the oven at low temperature after being washed quickly was the best, the effect of fermented bacteria fertilizer was the best and the second was k2so4 compound fertilizer and k2so4 fertilizer, the contents of carbohydrate components and extracts of radix glehniae " baiyintiao " were the highest and the content of imperatorin of radix glehniae " dahongpao1 was the highest, the quality of radix glehniae during blooming or seeds setting period was worse, the quality of radix glehniae planted in hucheng laiyang was better than those planted in anguo hebei and inner mongolia

    結果：通過對歐前胡素、糖類成分、氨基酸、浸出物、灰分和水分的分析測定，結果表明：北沙參採收以10月份為宜；藥材加工以趁鮮水洗、帶皮切片曬干或低溫烘乾最佳；追肥以酵素菌高效生物肥效果最好，其次為硫酸鉀復合肥、硫酸鉀；栽培品種「白銀條」的糖類成分和浸出物含量最高，而「大紅袍」的歐前胡素含量最高；當年開花和當年結種的北沙參質量較差；北沙參藥材質量以萊陽胡城產最佳，而河北安國和內蒙古產的則較差。
  11. Nutritional analysis on metal elements in ferment fertilizer

    酵素菌肥的金屬元素營養分析
  12. Super - oxide - dismutase tea contains s. o. d. enzyme and essential minerals. japan food analysis centre did an analysis in this compound and confirmed that it contains valuable minerals and enzymes

    日本食品分析中心化驗分析，證明s . o . d抗酸茶含有s . o . d酵素及大量人體所需的礦物質。
  13. Batch process data can be arranged in a three - way matrix ( batch x variable x time ), and the process of antibiotic fermentation is a typical batch process

    將多元統計分析方法應用於發酵過程性能監控。間歇過程的數據可以用三維數組來表示，抗生素發酵過程是一個間歇過程。
  14. The aim of the software is to optimize and control the operation of fermentation process. the dissertation focuses on : 1. the kinds of parameters measured in fermentation process are analyzed

    分析了抗生素發酵過程中參數的類型，如按照性質可以分為：物理參數，化學參數，生物參數等，按照測量方法可以分為：在線測量參數，離線測量參數和軟測量參數。
  15. Having about 10, 000 sets of advanced manufacturing machines and analytical instruments, 7000 cubic meters fermentation tonnage and brand - new gmp approved workshops for solid and liquor finished dosage forms and apis, producing more than 350 varieties and specifications of all kinds of medical products, adding to complete quality analysis and quality assurance system, nationwide sales net work and fast - growing international business, topfond is playing a more and more important role in chinese pharmaceutical industry and in the world as well

    公司擁有國內外先進生產設備、分析儀器近萬臺（套） ， 7000立方米的抗生素發酵噸位，嶄新的針劑、片劑及原料藥gmp車間，生產350多個不同規格類型的產品，具有完善的質量檢驗和質量保證系統，銷售網路覆蓋全國，並積極向全球拓展，所有這些都表明天方在中國乃至全球醫藥行業正在起著越來越重要的作用。
  16. The advances on bio - pesticide mildiomycin and its analogs were reviewed, and their fermentation technology, mechanism of synthesis and action, separation technology, analysis technology and application based on our research were introduced at the same time

    摘要從發酵技術、合成機理、分離技術以及分析檢測技術等幾個方面結合本實驗室的研究工作詳細介紹了米多黴素及其衍生物的國內外研究進展。
  17. The characteristics of natural cellulose were analyzed, the key techniques ( pretreatment, saccharification and fermentation etc. ) of bio - fuel alcohol by different kinds of cellulose were compared, the present status of bio - fuel alcohol production by cellulose was summed up, and its development trend was discussed

    摘要分析天然纖維素的資源特徵，比較各種纖維素生物燃料酒精生產工藝中的預處理、糖化和發酵等關鍵工藝環節的技術特點，綜述了以纖維素為原料生產纖維素生物燃料酒精技術的現狀，討論了該技術的發展方向。
  18. A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml

    本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。
  19. This project is aimed at : ( 1 ) expressing melittin and megf in e. coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake

    本課題以大腸桿菌（ e . coli ）宿主重組表達melittin和megf ；以melittin為毒性部分、 megf為靶向部分，構建膜毒性免疫毒素；用定量競爭rt - pcr法測定megf體內表達豐度；檢驗所構建的各重組菌株的遺傳穩定性；採用搖瓶分析對各重組菌株的發酵條件進行了初步優化。
  20. To accelerate the time - consuming analytical procedure involving 2 - 5 days of biological testing, we employed a real - time pcr protocol in conjunction with the personal sampler for collection of airborne viruses

    為減少2 5天生化分析所需時間，本研究使用即時定量聚合酵素反應技術結合個人采樣器採集空氣中病毒。
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