酵素化學 的英文怎麼說
中文拼音 [jiàosùhuàxué]
酵素化學
英文
enzyme chemistry-
Dark green leafy vegetables such as broccoli, spinach, collards, turnip greens and yellow vegetables and fruits such as apricots are rich in biochemical substances that protect vision from one of the major factors in age - related macular degeneration, say richard bone, professor of physics, and john landrum, associate professor of chemistry, in the latest issue of the journal methods in enzymology
物理教授理察柏恩及化學副教授約翰蘭卓姆最近在酵素學方法期刊上發表指出，深綠色葉子的蔬菜，像花椰菜菠菜芥藍大頭菜，還有黃色的蔬菜和水果，像是杏桃，都含有一些豐富的生化物質，這些生化物質是對抗中老年視斑性退化的一個主要因素。Fundamental topics include why and when one may choose to use biological systems for chemical conversion, considerations for using free enzymes versus whole cells, and issues related to design and development of bioconversion processes
基本的主題包括:為何及何時可能選擇使用生物系統來進行化學轉化、考量使用分離酵素或完整細胞，以及與設計和開發生物轉化程序相關的議題。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The cytogenetic tests deal with all chromosomal analysis for different diseases. the molecular tests investigate about 40 genetic diseases and the biochemical tests mainly investigate enzymes and proteins products of genetic diseases
細胞染色體分析對不同疾病進行染色體分析，分子遺傳學檢驗對大約40種的遺傳疾病進行測試，而生化遺傳檢驗則主要檢驗病人體內酵素及蛋白質的含量，以測試有關的遺傳疾病。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。To explain the nature of food as a medium for chemical reactions and the effects of electrolytes, non - electrolytes, ph and dispersed phases on the structures and reactivities of food components ; to examine in detail the mechanisms of non - enzymic browning, food additive - food component reactions, and methods of control
介紹食物的化學反應、電解質與非電解質的效果、食物元素的酸堿值發散階段及組織與反應、非酵素褐化的結構、食品添加物成分與反應、與控制方法等。The physical, chemical and biological pretreatment, enzyme saccharification, alcohol fermentation technology and research development were stated in this paper
該文簡述了纖維素物理、化學、生物預處理、酶糖化、發酵酒精生產工藝及研究進展。The technologies for producing the synthetic - carotene chemically and natural product based on c14 - aldehyde and vitamin a are introduced, including the method of extraction from natural plants, silkworm feces, salt alga and the method of microbe ferment
摘要綜述了-胡蘿卜素的生產方法，介紹了以c14醛為原料和以維生素a為原料的化學合成品生產方法和天然品的生產方法，包括從天然植物、蠶糞、鹽藻中提取和微生物發酵法。Characterization of waste - digestion for subsequent determination of aqua regia soluble portion of elements
廢棄物的特徵.化學元素的王水溶解部分的系列測定用發酵法The aim of the software is to optimize and control the operation of fermentation process. the dissertation focuses on : 1. the kinds of parameters measured in fermentation process are analyzed
分析了抗生素發酵過程中參數的類型，如按照性質可以分為：物理參數，化學參數，生物參數等，按照測量方法可以分為：在線測量參數，離線測量參數和軟測量參數。Phosphatase enzymes expedite the breakdown of phosphate monoesters in about 10 milliseconds. without the enzyme, the reaction ' s half - life would be a trillion years
五、磷酸酶這種酵素，可以使磷酸單酯迅速在千分之一秒內完成分解。若沒有這個酵素，這個化學反應的半衰期會是一萬億年。Marine biology research center in woods hole, massachusetts. includes organism index, articles, essays and images
-主題為對分解海洋污染物或油污微生物之研究海洋環境與污染物對海洋環境之影響微生物生態酵素化學“ in the future, research in molecular biology may provide improved techniques for manipulating macromolecules, ” adleman wrote in a seminal 1994 scientific paper describing the dna experiment. “ research in chemistry may allow for the development of synthetic designer enzymes
1994年，艾德曼在這篇說明他啟發性的dna實驗科學論文中寫到：未來，分子生物學的研究成果，將可改良操控巨分子的技術；化學方面的研究將可促成合成設計酵素的發展。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。" green tea contains antioxidants 20 times more powerful than vitamin e, and 500 times more powerful than vitamin c, at protecting the body from cancer, " says mitchell gaynor, m. d. and researchers say it also inhibits an enzyme called cox - 2, which tumors need to grow
米邱蓋挪醫師說：綠茶含有抗氧化物能預防癌癥的產生，功效是維它命的二十倍，是維它命的五百倍。研究學者也指出：綠茶可以抑制一種酵素，叫做cox - 2 ，此一酵素是腫瘤生長所需的物質。分享友人