酶命名法 的英文怎麼說
中文拼音 [mìngmíngfǎ]
酶命名法
英文
enzyme nomenclature-
The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。Huwentoxin xi ( hwtx ~ xi ), a serine protease ! inhibitor, consists of 55 amino acid residues with three disulfide bridges. the toxin was isolated from the venom of the chinese spider ornithochoctonus huwena by ion - exchange chromatogram - phy and reverse phase high performance liquid chromatography
本文報道從虎紋捕鳥蛛（ ornithochoctonushuwena ）粗毒中，應用陽離子交換和反相高效液相色譜的方法分離到一種胰蛋白酶抑制劑，命名為huwentoxin - （ hwtx - ） 。The construction of pci - il - 2 : primers were designed by the software oligo, xhol and sail were added to the primers, the cdna of il - 2 was obtained by pcr. the cdna of il - 2 was ligated with pci - neo cleaved by xhol and sail. the recombinant was evaluated by pcr
Pcr法擴增幾億片段，在t4連接酶的作用下與xhol和sal工酶切的pci neo載體連接， pcr法鑒定重組體，用xho工， xhol sal ， ban工工酶切進一步驗證重組體，命名為pclll 2 。
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