酶消化 的英文怎麼說

中文拼音 [xiāohuà]
酶消化 英文
enzymatic digestion
  • : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
  • : 動詞1 (消失) disappear; vanish 2 (使消失; 消除) eliminate; dispel; remove 3 (度過; 消遣) pa...
  • 消化 : digestion; digest
  1. This charge is reduced by digestion with neuraminidase.

    這一電荷被唾液酸苷酶消化而降低。
  2. In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test

    在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析,證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列,在iptg誘導下兩種嵌合分子都獲得了分泌表達,表達產物主要集中在細胞周質空間。
  4. Soya peptone is an enzyme digest of soya, this product contains carbohydrate, widely used in culture media and often used for the cultvation of many fastidious organisms and where rapid, luxuriant growth is required

    性狀:本品系由大豆為原料,經酶消化而成,富含碳水合物,廣泛應用於微生物培養基的配製並常用於營養要求高的微生物培養及滿足微生物快速大量生長的需要。
  5. To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau

    摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法,採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞,取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度;臺盼藍排斥試驗檢測活細胞數。
  6. The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6

    方法無菌條件下,從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織,採用組織塊培養法和酶消化法分離滑膜細胞。
  7. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法:胰島的分離參照王玲等的方法,每次實驗取新生3 - 5天sd大鼠,無菌條件下剖腹取出胰腺,剪切為0 . 5mm ~ 3的組織塊, v型膠原酶消化30min后,離心洗滌,懸浮於完全培養基,接種入50ml培養瓶,於5 co _ 2 、 95空氣條件下培養20h ,轉板純,接種於96孔培養板培養24h ,按實驗要求進行實驗。
  8. The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time

    石蠟切片進行原位pcr檢,預處理分別滴加loom歲血的蛋白k20閃,以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準,確定最適時間『 , ,研究不同固定時間與蛋白酶消化的相互影響和關系,同時檢測石蠟切片的mn基因型。
  9. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上,構建了4個全長基因的分子克隆,分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ,其中兩個轉移到低拷貝載體plg338上,命名為plgd30343 、 plgd70344 。
  10. At last, ctab - dna and sds - dna methods are used in this experiment. on the basis of optimizing experiment procedure and pcr system of the materials, the initial reversion transcription system and orthologus gene cloning technique are established

    對這3種方法比較后,確定本實驗採用ctab - dna酶消化法和sds - dna酶消化法提取白樺雄花芽組織的總rna 。
  11. Since there are more things in the bud tissue of the male flower, such as phenolic compounds, polysacchrides, proteins and some other secondary metabolites, three methods used to isolate rna are tested in this assay, which are the enzyme digesting methods of ctab, ctab - dna and sds - dna

    克服了常規方法和試劑盒無法提取出富含酚類合物、多糖和一些尚無法確定的次級代謝產物的白樺花芽組織rna的障礙,提出了3種白樺雄花芽組織rna的提取方法: ctab 、 ctab - dna酶消化法和sds - dna酶消化法。
  12. The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum

    應用胰酶消化法進行頜下腺細胞的分離、純及原代培養、傳代培養。
  13. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 ( 7一36 ) nhz ,實驗組培養液中含有20nmol / lglp一1 ( 7一36 ) nhz ,培養24h后,用0 . 25 %胰蛋白酶消化胰島分散細胞,塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交, dab顯色,高清晰度病理圖文分析系統( highpathologiealimageanalysissystem , hp認s )對細胞著色的平均光密度( mean即tiealdensity , mod )量分析,觀察實驗組和對照組胰島素mrna的表達情況。
  14. Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum

    含胎牛血清膠原酶消化法培養兔成骨細胞
  15. Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes

    結論:酶消化法原代培養牛視網膜周細胞是一種穩定、可靠的方法。
  16. Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared

    方法採用不同的膠原酶消化法及不連續密度梯度純法。
  17. In this experiment, the method of trypsin digestion was improved, and that several kinds of mouse fibroblasts and porcine fibroblasts were successfully dissociated and cultured

    實驗中改進了胰蛋白酶消化法,成功地分離和培養了多種鼠成纖維細胞和多種豬成纖維細胞。
  18. This research use high sensitive, special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time, by detecting the genotype of mn blood group of the organizes slices. at the same time, we study the research of main influence factors, such as protease digestion, etc. we hope to set up a kind of steady, practical methods to detect the genotype of the material treated by paraffin wax and formlin, offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence

    本研究應用靈敏度高、特異性好的原位pcr技術,測定福爾馬林固定不同時間的石蠟切片組織mn血型的基因型,並對蛋白酶消化時間等主要影響因素進行了初步的研究,以建立一種穩定、實用的檢測石蠟組織切片dna遺傳標記的方法,為法醫物證鑒定和個人識別提供一種新的檢測手段。
  19. As a result, more than 97 % of the testicular cells remained their viability after enzymatic digestion

    結果組合酶消化后獲得的睪丸細胞懸液中, 97以上的細胞仍然存活。
  20. 2 ) hanks " buffer of trypsin not being added edta could obtain better the growth potential and the subsequent attachment of harvested skin cells t then it being added edta have better digested potent

    與添加edta相比,用只含胰蛋白的hanks '液處理貼壁細胞,所回收的傳代細胞存活能力較好;而添加edta的胰蛋白酶消化能力較強。
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