酶解物 的英文怎麼說
中文拼音 [jiěwù]
酶解物
英文
substrate-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先,用~ ( 35 ) s標記的氨基酸混合物喂養工程菌成功地制備了~ ( 35 ) s標記的擬南芥鈣調素( ~ ( 35 ) s - cam ) , ~ ( 35 ) s - cam純度高、放射活度高、 ca ~ ( 2 + )與egta存在時的電泳行為與未標記cam相同,可作為一種高靈敏性的探針用於進行受體學分析實驗;用擬南芥種子誘導愈傷,通過酶解制備了大量原生質體。Study on technology for hydrolysis of chrysalis ' s protein and its products ' analysis
酶解蠶蛹蛋白工藝的研究及產物分析Hplc determination of inulin zymohydrolysis products
高效液相色譜法測定菊粉酶解產物Influences of feeding lyophilized royal jelly on immune status in mice
酶解雞蛋清小肽混合物對小鼠免疫功能的影響Casein of dairy is the important sources of those peptides and can release them during gastronintestinal digestion, or enzyme proteolysis
摘要酪蛋白是生物活性肽的重要來源,可以通過體內的胃腸消化和食品加工過程中的酶解將其釋放出來。For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development
從中隨機選擇一些差異蛋白質點,進行基質輔助激光解吸-電離飛行時間質譜( maldi - tof - ms )測定其膠內酶解后的肽質指紋圖譜,用mascot軟體查詢swiss - port數據庫,初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質,如erp60 、 trypsinogen 、 proelastase 、 lipase等。Particularly advantageous appears the ability of the present method to fine - tune the separation of smallsize fragments and tryptic digests, where conventional sds - page usually fails
該方法在傳統sds - page通常不適用的小的碎片和胰酶解物的分離表現出特別的優勢。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Study on functionality of controlled enzymolysis hydrolysate from harengula zunasi by complicated enzymes
青鱗魚蛋白復合酶控制水解物功能特性的研究The experimental results suggest that the cell disruption ratio can reach 97. 88 % using enzymolysis and nanometer - crushing machine, and the contents of amino acid in the extractives are also considerably raised
試驗結果表明,酵母細胞先經酶解再結合納米破碎機破碎,可使酵母細胞壁的破碎率達到97 . 88 % ,大幅度提高了抽提物中氨基酸的含量。There are six systems of biohydrogen production which include biophotolysis, organic degradation of light, hydrogen synthesis via the water - gas shift reaction of photoheterotrophic bacteria, hybrid system of photo - fermentation, anaerobic fermentation and in vitro hydrogen production by hydrogenase
討論了光合成生物制氫系統、光分解生物制氫系統、水氣交換反應生物制氫系統、光合發酵雜交生物制氫系統和厭氧發酵生物制氫系統、離體氫酶生物制氮系統等6個生物制氫系統。In this paper, it was summarized that effectiveness of an ultrafiltration device, different sizes of an ultrafiltration device, the concentration of dietary enzyme - hydrolysed casein, animal trial process and results of using enzymehydrolysed casein, respectively, on enzyme - hydrolysed casein method for determining endogenous ileal amino acid excretion in the pig
摘要分別從超濾管用於酶解酪蛋白測定的可行性、不同規格超濾管對酶解酪蛋白測定的影響、酶解酪蛋白的適宜添加量、酶解酪蛋白應用的動物試驗步驟以及酶解酪蛋白測定效果等方面,對酶解酪蛋白方法測定豬回腸內源性氨基酸排泄量進行了綜述。Studies on angiotensin i - converting enzyme inhibitory peptides from acetes chinensis protein hydrolysate
中國毛蝦蛋白水解物中血管緊張素轉移酶抑制肽的研究The results demonstrated that they can increase the organic immune function and are good nonspecific immunity activators
結果提示:玉米胚芽蛋白酶解物能提高機體的免疫功能,是一種很好的非特異性免疫激活劑。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體形成和再生的主要因素,確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶菌酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生菌落;後者採用二級菌絲培養,用1mg ml的溶菌酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生菌。The seed dipping and foliage test on some vegetable with liquid seaweed manure prepared by acid - degradation and enzyme degradation showed that the liquid seaweed manure could enhance seed germination rate, promote crop growth, increase production and improve quality
摘要採用由酶解法和酸解法制得的海藻液體肥,在蔬菜作物上進行浸種和葉面噴灑試驗,結果表明:海藻液體肥能顯著提高種子發芽率,促進作物的生長發育,提高作物產量,改善作物品質。Separation and purification of baicalein from enzyme reaction
酶解產物黃芩素的分離提純Study on angiotensin converting enzyme inhibitory peptides from digestion of soy protein in vitro
大豆蛋白體外酶解物中血管緊張素轉化酶抑制劑活性肽研究Corn germ protein was hydrolyzed by trypsin and the effects of hydrolysates on mouse immunity were investigated
用胰蛋白酶水解玉米胚芽蛋白,研究了玉米胚芽蛋白酶解物對小鼠體內的免疫調節作用。The results of the experiments showed that hydrolysates can remarkably enhance the thymus and spleen index, the phagocytsis index and phagocytsis percent of macrophage, and the lymphocyte transformation for normal mouse while also promote the form of hemolysin in mouse body
研究結果表明,玉米胚芽蛋白酶解物能顯著提高正常小鼠的免疫臟器指數、腹腔巨噬細胞的吞噬百分率、吞噬指數和淋巴細胞的轉化功能活性,促進溶血素的形成。分享友人