醇酵母 的英文怎麼說
中文拼音 [chúnjiàomǔ]
醇酵母
英文
alcohol yeast-
The effects of pressure fermentation on barm propagation, the formation and reduction of diacetyl, the formation of higher alcohols and esters, and beer taste were studied in the experiments
摘要實驗研究了壓力發酵對酵母繁殖、雙乙酰形成及還原、高級醇和酯的形成,以及啤酒口味等方面的影響。The study on optimal control of yeast fed - batch fermentation for ergosterol production
酵母生產麥角固醇流加培養過程的優化Effect of over - expression of sterol c - 22 desaturase on ergosterol production in yeast strains
22去飽和酶高表達對酵母細胞麥角甾醇合成的影響Construction of intergeneric yeast fusant for production sorbitol from jerusalem artichoke
利用菊粉產山梨醇酵母融合菌株的構建Screening of low higher - alcohols and diacetyl producing saccharomyces cerevisiae swain with ultraviolet mutagenesis
紫外誘變篩選低高級醇和雙乙酰含量的啤酒酵母Effect of buffer capacity of saccharified wort on the yield of higher alcohols in beer brewing
麥汁緩沖性能對啤酒酵母高級醇生成量的影響The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體,然後導入畢赤酵母( p8chianastoris )菌株gslls細胞中,在甲醇的誘導下,經過酵母高密度發酵進行pap的表達,經sds page分析,結果表明,在培養基上清液中含有一明顯的特異性蛋臼條帶,大小為34ku ,經western blotting分析,該蛋白與法國pap抗血清有特異性反應,體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性,說明該pap基因在畢赤酵母gs中也得到了正確表達。It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上,經過甲醇誘導之後,該基因得到了分泌表達。This study gives the prime reference for the new technology of alcoholic fermentation with immobilized yeast coupling with extractive solvent
為固定化酒精酵母乙醇發酵與溶劑萃取耦合新工藝的開發研究提供初步資料。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。The main research contents and results are as follows : ( 1 ) disc papers and oxford cups were used to ascertain bacteriostatic effect of hongqu. using 70 % ethanol and water as solvent, the extraction inhibited bacillus subtilis, bacillus cereus and staphylococcus aureus and had no effect on e. coli, salmonella. typhi, yeasts and algae
主要研究內容和結果如下: ( 1 )利用濾紙片法和管碟法確定了紅曲的抑菌譜:紅曲70乙醇和水溶液浸提物對蠟樣芽孢桿菌、枯草芽孢桿菌等革蘭氏陽性菌具有較強的抑制作用,對革蘭氏陰性菌、黴菌和酵母菌沒有抑制效果。The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中,經電激轉化至畢氏酵母菌株gs15中,再經甲醇誘導,在150ml搖瓶畔1獲得細胞外分泌表達產物。Gap is a homogenic microencapsule consisting of a consolidated nutrient and is not a mixture. its ingredient include ; whole egg, shrimp head extract, casein, yeast extract, cholesterol, lecithin, enzyme, polyunstaurated fatty acid, trace minerals, vitamins
酪蛋白鮮蛋液魚肉蛋白分解物酵母抽取物幾丁質膽固醇磷酯脂酵素高級不飽和脂肪酸epa微量礦物質綜合維他命及其他未知生長因素u . g . f 。Mutation and screening of fuel ethanol producer through 96 - well plantes high through put screening
96孔板高通量選育產乙醇酵母Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page電泳分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的條帶。Zymosn in the kangsheng multi - nutriment is consist of gluconsan and mannan. zymosan is the most active glucosan in life - bodies, which has the function of human immunity, anti - cancer, anti - inflammation, anti - radiation and reduce the cholesterol
康聖天元多價營養素中酵母多糖由葡聚糖和甘露聚糖組成,酵母葡聚糖是生物活性最強的葡聚湯,具有人體免疫、抗腫瘤、抗炎、抗輻射和降低膽固醇的作用。Many recombinant hydroxynitrile lyases have been expressed in escherichia coli, saccharoinyces cerevisiae and pichia pastoris
許多重組醇腈酶業已在大腸桿菌,啤酒酵母和甲醇酵母中成功表達。Expression of gene coding mature peptide of sag1 of toxoplasma gondii in yeast pichia methanolica
1成熟蛋白編碼區基因在甲醇酵母中的初步表達The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine. multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol. the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified
轉化嗜甲醇酵母獲得耐受4mg mlg418的菌株,經sds - page電泳和westernblotting檢測表明其上清培養液中的蛋白可與猴抗pzp3抗體和兔抗hcg抗體發生特異性的反應。For the pure ple has the better activity, a procedure is designed in this research, that is to construct the engineering microorganism to express the ple efficiently. methylotrophic yeast expression system has been developed quickly recently. many proteins are expressed by it in foreign countries
甲醇酵母表達系統是最近迅速發展起來的一種真核表達系統,它能以甲醇為唯一碳源大量合成蛋白質,已用於表達多種外源蛋白,被認為是最具發展前景的蛋白質生產工具之一。分享友人