重組活化基因 的英文怎麼說

中文拼音 [zhòngzǔhuóhuàjīyīn]
重組活化基因 英文
recombination activating gene
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 重組 : bpr
  • 活化 : [化學] activation radioactivation; activate活化處理 activating treatment; 活化分析 [核物理] activ...
  1. Epidermal growth factor ( eof ), a single - chain polypeptide of 53 - amino acid, which was firstly isolated from the submaxillary gland of mice in 1962, has wide potent applications

    人表皮生長因子（ hegf ）是從人尿中提取的由53個氨基酸組成的小分子活性多肽，是促進細胞增殖和分化至關重要的生長因子。
  2. By means of historical literature, the author analyzed the achievements made and insufficiencies existed in rural sports practice in china since the establishment of china, and put forward the following opinions based on his analysis : for the development of rural sports practice in china in the future, governmental functions should be intensified, and the mode of governmental functions should be institutionalized, standardized and legalized ; the construction of rural fundamental sports organizations should be strengthened, and rural fundamental sports activists should be cultivated ; local and national sports activity events should be fully explored, coordinated and promoted, so as to enrich rural sports activity contents ; rural sports activities should be properly developed according to the time, location, people and program, so as to add new contents to the celebration of traditional rural holidays ; attention should he paid to the issue of development of sports for disadvantageous groups and migrant laborers in rural society ; attention should also be paid to rural scholastic physical education and full utilization of rural scholastic physical education resources

    摘要通過歷史文獻，分析了建國以來中國農村體育實踐所取得的成就和不足，在此基礎上提出：未來中國農村體育的發展要強化政府的作用，政府的作用方式要制度化、規范化和法制化；要加強農村體育基層組織建設，培育農村基層體育骨幹；要充分挖掘、整理和推廣地方性、民族性的體育活動項目，豐富農村體育活動內容；要因時、因地、因人、因項目制宜地開展農村體育活動，為農村的傳統節日慶典注入新內容；重視農村社會弱勢群體和異地民工的體育發展問題；重視農村學校體育，充分利用農村學校體育資源。
  3. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  4. By applying the theory and method of geological anomaly, it is focused on the analysis of the characters of major geological anomalies and their effect of mineralization in daye - jiurui region. the analysis result shows that the basement fault anomalies control a few major large hidden batholiths and their associated orefields ; that the anomalies of cover structure confine most cu - au deposits and fe - cu deposits within their scope ; that the geochemical anomalies and lithofacies - paleogeographical anomalies are major geological factors leading to trataboundness of some cu - au deposits ; that the anomalies of rock operties provide the favorable chemical and physical conditions for the ore - forming process, including the seepage, circulation, convergence and deposit of ore - bearing hydrothermal solution ; that the anomalous ctive stage of mesozoic magmatite is almost consistent with the major mineralization epoch of the fe, cu deposits, and the structure of multi - levels, and the zoning in magmatic system determine the spatial distribution of the mineralization system. then, based on the above analysis, a comprehensive variate, geological combination entropy, is brought forward for reflecting the complex degree of the combination of geological ore - controlling factors. after circumscribing the anomalies of geological combination entropy, the relations between the anomalies and ore deposits are determined

    從地質異常的角度，重點分析了大冶九瑞地區主要地質異常的特徵及其對成礦的影響.由分析可知：基底斷裂異常控制了本區若干重要規模較大的隱伏巖基及伴生礦田，而蓋層構造異常控制銅金礦床和鐵銅礦床的空間位置；地層的地球化學異常和巖相古地理異常是造成一些銅金礦床層控性的主要地質因素；蓋層的巖性異常為含礦熱液的滲流、循環、聚集和礦質沉澱等一系列成礦作用提供了有利的物理和化學條件；中生代巖漿巖的異常活動期也是本區主要的鐵銅成礦期，巖漿巖系統的多層分枝和分帶性結構控制了本區成礦系統的三維空間分佈.在上述分析的基礎上，構置了地質組合熵作為反映控礦地質因素組合系統結構復雜程度的綜合變量，並圈定出組合熵異常，由此確定了綜合地質異常與礦床的關系
  5. The main issues of the research are put as following : firstly, with the international comparing of market sharing rate and trading competitive index, it was revealed that wheat in china has inferior international competitiveness with a bit rising during current years, however, which is still behind that of the main wheat export countries. secondly, after the international comparing of the main factors that affect the international competitiveness of wheat, it was discovered that chinese wheat has the obvious cost advantage on unit product, while because of the high circulation fee, it results in inferior advantage on the price ; low and unstable quality is another factor which leads to inferior wheat competitiveness ; the input of fertilizer and labor makes little impact on the productivity of chinese wheat, while the input of seeds, irrigation and machine makes a strong impact, so it should be more invested in seeds, irrigation and machine to reduce wheat ' s unit cost. the assistant industries of the wheat, such as breed, production materials and processing industries, have inferior international competitiveness and lagged development

    其次，通過對影響小麥國際競爭力的主要因素的國際比較發現：中國小麥單位產品生產成本具有明顯優勢，但由於較高的流通費用，導致在價格上不具有優勢；小麥質量較差、品質不穩定是導致中國小麥國際競爭力較低的主要因素；生產要素中化肥和勞動力投入對中國小麥生產力水平的影響程度較小，而種子、灌溉和機械投入對小麥生產力水平的的影響程度較大，因此小麥生產投入要以增加種子、灌溉和機械的投入為主，代替大量的化肥和勞動力投入，進一步降低小麥單位產品成本，增強中國小麥國際競爭力；中國小麥的上下游輔助產業（包括品種資源、生產資料和加工業）的國際競爭力較弱，發展較為滯后；中國小麥生產者的組織化程度較低嚴重製約了中國小麥質量的提高、流通費用的降低和加工業的發展；小麥生產經營活動本身的特點決定了在充分發揮市場機製作用的基礎上，必須通過政府的宏觀調控來克服其市場機制的失靈，保障市場機制有效運行，但通過國際比較研究發現：中國政府在生產者支持、市場體系建設和國際貿易政策上對小麥的支持水平較低，與提高中國小麥國際競爭力的要求有較大差距，尤其是較低的生產者支持水平和市場體系建設程度制約了中國小麥國際競爭力的提高。
  6. Chemical industry information not merely embodyed the ordinary chemistry information substance, chemistry manufacture technological process and representative installation introduction are still have, in case contacing legal institutions sulphuric acid ? nitric acid industry system law ( ammonia oxidate law ), synthetic ammonia industry, chlorine and caustic soda are manufacture to electrolysis salt water, along with electrogilding, refine aluminium, iron - smelting and steel - smelting, cement and glass manufacture summarized account, the mineral oil is refined, hence, the chemical industry information education is contrastd against the ordinary chemistry information education, proper such representative means, be living, the means interpreted is in speech oridinarily wholly adoptd in the chemical industry information education, now most of tutors also is adopt the means interpreted in speech, cause that the chemical industry information education is insipid like this, student lack interest to chemical industry information study, but as a result of value the pair foundation, light practice, student may say the become a mere formality to chemical industry information study o since multi - med

    化工知識不僅包含了一般化學知識的內容，還帶有化學生產工藝流程及典型設備的介紹，如接觸法制硫酸，硝酸的工業製法（氨氧化法） ，合成氨工業，電解食鹽水製造氯氣和燒堿，以及電鍍、煉鋁、煉鐵和煉鋼，水泥和玻璃的生產簡介，石油的煉制等，因此，化工知識教學與一般化學知識教學相比，應有其典型的方法。在過去，化工知識的教學一般都採用口頭講解的方法，現在大多數教師也是採用口頭講解的方法，這樣使得化工知識的教學乏味，學生對化工知識的學習缺乏興趣，又因重視雙基，輕實踐，學生對化工知識的學習可以說流於形式。由於多媒體的輔助教學的高效性、形象直觀性、新穎性和多樣性、人工模擬等特點，很適合化工知識的教學，應用多媒體教學化工知識可以解決目前存在的諸多問題，如：模型短缺、組織參觀活動困難、教育經費不足、學生不易觀察等。
  7. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  8. The chinese university of hong kong has entered into a research agreement with genius international holdings limited to develop a technique to produce bone morphogenetic proteins ( bmps ) as a therapeutic device for bone fractures and defects. the new technique will involve the utilization of recombinant dna and protein engineering technology

    香港中文大學和雅智國際集團有限公司（ geniusinternationalholdingslimited ）已達成合作協議，應用基因工程、蛋白工程及生化科學等多學科技術，開發及生產具有成骨生物活性的重組骨蛋白- 2 （ bonemorphogeneticprotein - 2 ， bmp - 2 ） ，以應用於治療骨折及骨損傷。
  9. The article intend to analysis the precess, character and motivion of development in traditional street space in which contain abundant cultural heritage. through investigation the writer should analysis the characte r, form and the relationship with each other, and furthermore find the pattern of the space in the fulture. what the most important is to summarize the spitits in traditional street space, such as activition, fuction, cultural elements, social customs and quatity of space. in order to put these elements to the exploit of new street space and find how these elements should be used, the writer regards some streets as the examples such as wenming street in kunming, xinhua street in lijiang jianshe rode in luxi. through research the writer find problems which exist in not only traditional but also in new street and express individual viewpoint about how to solve the problems. there are several ways to keep the new street exploition success. in the first place, we shoule know about all kinds of influences and synthesized elements. secondly mastering the mechanism of the development by oneself is indispensable. in the last place, it is necessory not to imitate some certain model which is plausible in other place. in the l ast of the article, the writer uses a new project to further illustrate the way to put the theory what have mentioned above into the practice

    並從自然，社會，經濟及社會文化方面分析產生的根源，發展動力，其次通過實際調查，對現有傳統商住的物質形態要素進行分析，從商空間及住空間的基本特徵，型制入手，探索傳統商住街區的空間形態，結構組成，商與住空間關系，空間性質及空間如何相互轉化。以此來尋找傳統商住街區物質形態特徵，同時結合具體實例強調在傳統商住街區改造、新區的規劃設計中應當重視非物質要素內涵的提取、如活動者及社會結構、功能意義、空間品質、文化氣息，保持歷史傳統的延續性的必要性與可行性。在文章的後半部分，有針對性的對遺留下來的傳統商住街區-昆明文明街、麗江新華街、瀘西建設路的物質形態特徵和非物質形態特徵的分析，找出現存的問題，並針對問題發表個人的觀點，指出引導現代商住街區的開發與改造中，保持形態合理發展的正確途徑是了解影響形態發展與演變的各種因素及其綜合作用，把握好形態演交的內在機制，而不應刻意追求某種特定的模式。
  10. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  11. The complicated life cycle of streptomyccs is one of the most important reason that attracts researchers on its genetics. a great deal of valuable information on the morphological, physiological differentiation of streptomyccs coclicolor will emerge after genome sequencing is completed. based on the s. coelicolor dna sequences that have been finished and focusing on three ftsk homologous, which may be closely related with cell division, this study tried to find out the functions of three ftsk homologues genes by gene replacement or disruption

    鏈黴菌復雜的生活史是研究其遺傳學的一個重要原因，天藍色鏈黴菌全基因組測序將會為其形態及生理分化發育的研究提供大量有用的信息，本研究基於天藍色鏈黴菌已測出的98序列，對一組可能與細胞分裂有關的基因? ftdk同源基因? ?進行基因置換或中斷並對其功能進行初步研究。
  12. The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination

    為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。
  13. The management innovation is the key factor of improving the ability of competition and plays an essential role. the management innovation benefits the profits, the costs, the management level, the innovations and the corporation cultures of enterprises. it forms efficient effect mechanism and improves and enforces the ability of competition of enterprises by the management of strategy, organizational structures, human resources and the technologies and methods of management control and the corporation cultures

    創新性地提出了：管理創新與管理是企業同一經營活動中，既相互聯系又相互區別的活動，也是企業經營成功的根本保證；管理創新是提升企業競爭力的關鍵因素，起著基礎和根本的保證作用；管理創新對企業的收益、成本、管理水平、企業家創新和企業文化改進等方面有著重要效用，它可以通過戰略管理、組織結構管理、人力資源管理、管理控制技術和方法以及企業文化等方面的創新，形成有效的作用機制，不斷提升和增強企業的競爭力。
  14. Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a

    我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。
  15. The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +

    主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。
  16. Under the induction by methanol, the expressed monellin was secreted into medium at the level of 0. 25g / l. the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris. shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen - poor conditions

    同時，採用畢赤酵母偏愛密碼子，人工合成了透明顫菌血紅蛋白基因（ vgb ） ；並構建了vgb基因的酵母胞內表達載體ppic3 . 5kv ，通過電擊轉化獲得了能在胞內表達有生物活性vhb的重組畢赤酵母gs115 / ppic3 . 5kv 。
  17. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
  18. The gene was subcloned into the pproextm htb vector, then was transformed the vector into e. coli dh5. a biologically active recombinant pil - 10 was obtained after induction by iptg

    然後將pil - 10編碼基因亞克隆到表達載體pproextmhtb中，轉化dh5后，用iptg誘導培養，表達了具有活性的重組pil - 10 。
  19. The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation

    Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
  20. From the ordinary developing rules of the outwarding enterprises, this thesis dissertates the relations between international trade and international division, the development tend of global economy unitizing and the multinational corporation, the reasons of why multinational corporation has developed so fast. associated with the theory of " gradual developing " of outwarding enterprisese, it points out : the main direction of our china outwarding enterprises is to build into powerful multinational corporation. from the analysis of the distributing structure of china outwarding enterprises, the main problems in these enterprise, especially for the function of foreign trading enterprises in development of our outwarding enterprises, it raises the main strategies of outwarding development of our china enterprises : foreign trading enterprises and productive enterprises could reach the goal of advantage - combination through incorporation and rebuilding which are based on capital management ; our china productive and foreign trading enterprises both should take brand strategy as orientation, and set up their brand competitive power ; foreign trading enterprises should strengthen the construction in their international marketing net and their role of enlarged services function ; it has also strengthened the importance of china outwarding enterprises to expand their scale, and foreign trading enterprises to expedite their way to go on stock market ; it also dissertates that international subdivision market is very large and complicated, it is the right place for our small outwarding enterprises to carry forward their energy

    本文從外向型企業發展的一般規律出發，闡述了國際分工與國際貿易的關系，全球經濟一體化的發展趨勢和跨國公司的巨大發展，分析了跨國公司發展的原因，結合外向型企業發展的「漸進性」理論，提出我國外向型企業發展的目標是組建有實力的跨國公司；同時根據我國外向型企業的分佈格局及目前存在的主要問題，特別關注了外貿企業在我國外向型企業發展進程中的作用，提出了我國企業外向型發展的策略：外貿企業與生產企業在資本運營的基礎上，通過兼并重組，達到優勢互補的目的；具有一定實力的大型生產企業和大型外貿企業要以品牌策略為導向，建立品牌競爭力；外貿企業應加強在建立國際營銷網，和擴展服務性功能等方面的建設；盡快擴大我國外向型企業的規模，加快外貿企業上市融資的步伐，組建新型企業集團；同時，本文還闡述了在廣闊的國際細分市場充分發揮我國外向型小企業的活力。
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