野生型基因 的英文怎麼說

中文拼音 [yěshēngxíngjīyīn]
野生型基因 英文
wild type gene
  • 野 : Ⅰ名詞1 （野外） open country; the open:曠野 open spaces [country]; wilderness; 田野 open fields; ...
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 野生 : wild; uncultivated; feral野生動植物 wilding; 野生蜂 wild bee; 野生生物資源保護 wildlife conservat...
  1. In contrast, transgenic mice overexpressing gat1 displayed ethanol tolerance, as shown by the righting reflex test. mi ce overexpressing gat1 survived a lethal dose of ethanol ( 9g / kg, i. p. ) longer, maintained locomotor activity longer after a sub - lethal dose ( 1. 75g / kg, i. p. )

    相反，在翻正實驗中過量表達gat1的轉基因小鼠表現出顯著的酒精耐受；與同胎所生的野生型小鼠相比，轉基因小鼠在酒精緻死劑量（ 9g / kg ， i . p . ）下存活較長時間；在酒精低劑量（ 1 . 75g / kg ， i . p . ）下維持更長時間的運動活力，相應的ld50也較高。
  2. Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines

    實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。
  3. Ddrt - pcr method was exploited to study the differential gene expression between immature siliques of arabidopsis wide - type and ast mutant

    採用ddrt - pcr的策略，分析野生型與突變型植株未成熟角果中基因表達的差異。
  4. Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

    含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。
  5. Wild type gene

    野生型基因
  6. Transgenic a rabidopsis plants overexpressioning gmdrebb display different phenotypes when compared to wild type

    轉gmdrebb基因的擬南芥在表型上與野生型有所不同。
  7. Hau3r gene of s. lividans 1326 is directly related to its resistance to actinophage hau3

    野生型變鉛青鏈黴菌1326中的hau3 ~ r基因與1326對噬菌體hau3的抗性直接相關。
  8. Accordingly, ers2 - 1 is still able to confer ethylene insensitivity via a single receptor gene ers1 in the quadruple mutant, but at a highly reduced level compared to its function in the triple mutants. the major difference between the quadruple and triple mutants is the absence of a wild - type ers2 gene in the quadruple mutant, and we propose that the dominance conferred by ers2 - 1 can be mediated and amplified via the wild - type ers2 to the subfamily i receptor ers1

    三突變體的遺傳背景與四突變體相比，只是在三突變體中保留了ers2的野生型基因，而當這個野生型的ers2基因突變后，對乙烯不敏感的ers2 - 1基因的功能便減弱了，說明顯性基因ers2 - 1在etr1 - 7 ； err2 - 3 ； ein4 - 4三突變體中的功能可以經由活化ers2后再傳給ers1 ，而不僅僅是直接傳給ers1 。
  9. The aim of this research is to establish a transgenic system for flower plants and investigate the functions of mapkk in transgenic plants in the future

    本研究構建了野生型、突變型mapkk基因植物表達載體，並用凍溶法將其導入了根癌農桿菌lba4404 。
  10. The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management

    重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。
  11. In the present study, an omanental flower sinningia speciosa was transformed with tmek2 and tmek2mut :, the wild type and mutant type of mapkk from tomato, by agrobacterium tumefaciens

    本研究用野生型、突變型mapkk基因轉化觀賞花卉大巖桐，為進一步研究mapkk基因的功能奠定基礎，也為花卉植物的轉基因提供實驗系統。
  12. The future characterization and genetic analysis for candidate mutant were carried out and find that some candidate mutant ( such as roi30 doil - 1 doi0311131 ) have good phenotype by drought h2o2 aba - stressed treatment. at the same time we also observe the development of candidate mutant at different growth stages carefully. many modal difference between mutant an d wild type at the same period were found, such as more rosette layering fatty and big in leaves, advancment or delay for the flower period, rosettes living in the main stem, shorten in figure, the amount of seed little, sterilization etc. these physiological and modal changes may reflect with maladjustment in expressions of some gene and confusion on their inner control, . we will futher study concrete and detailed function mechanism

    我們對這些擬南芥侯選突變體進行進一步的鑒定和遺傳學分析，發現ro口口、 doil 、 doi口jlll3i等潛在突變株對aba 、過氧化氫及早脅迫有明顯表型，同時對潛在突變體的生長發育進行了詳細的觀察，發現多數潛在突變株與同條件下野生型比出現了許多明顯的形態改變，如:蓮座基葉增多、分層、肥大，花期提前或延遲，主莖生輪座，株型矮化，產籽量少，不育，敗育等，這些生理和形態上的差異很可能反映了它們內部某些基因的表達受到了影響、代謝調控發生了紊亂，具體和詳細的作用機制還需要進一步的研究。
  13. When compared with kds301 ( lexa + ), xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc, indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans. deinococcus radiodurans " lexa had no revelance with its extremely radioresistance. the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter

    對lexa基因突變體xe的研究表明，其輻射抗性與lexa基因野生型的抗輻射菌kd8301相近，存活曲線上均顯示有一個寬大的肩區，結果發現lexa基因的突變並沒有改變抗輻射菌的獨特抗性，說明lexa基因與輻射抗性無關。
  14. The photoabsorption properties of three kinds of br molecule films ( the wild - type br, the chemical enhanced br, the gene - variant br ) are investigated by measuring their absorption spectrum. using the gene - variant br film as a light - modulator, by the experiment of write - readout image and the analysis of image contrast, the light - modulated property of the modulator is studied. the relationship between wavelength of the modulated light and response time of the modulator is discussed emphatically

    本論文概述了細菌視紫紅質分子結構、功能特性及研究發展的歷史與現狀，介紹了目前已有的和潛在的一些重要應用，通過對吸收光譜響應特性的測試研究了三類br分子薄膜（野生型，化學修飾型，基因修飾型）的光吸收特性，通過圖像的記錄/讀取實驗及圖像的對比度分析探討了基因改性型br分子薄膜的光調制特性，著重研究作為一個光調制器件對調制波長和調制時間的響應特性。
  15. The coding regions of wild bacillus thuringiensis cry1ie and cry1ac gene were modified according to plant preferring codons. the modified crylle and crylac gene were artificially synthesized

    本研究根據植物所偏愛密碼子原則，改造蘇雲金芽孢桿菌crylac ， crylie野生型基因的編碼區序列，人工合成crylac ， crylie基因。
  16. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  17. Pllz1112 was transformed into m145 to disrupt sc6a9. 34. a mutant named hxy1 was obtained and confirmed to be disrupted at correct site by southern blotting

    將phz1112導入野生型菌株m145中獲得基因中斷菌株hxy1並通過southern雜交驗證hxy1在正確位置發生了基因中斷。
  18. The dnd cluster seemed to express when it was carried by the high copy - number plasmid because complementation of individual dnda, dndb or dndc mutation could be observed and such expression did not seem to influence the normal dnd expression in wild type s. lividans 1326

    而在高拷貝質粒上的dnd基因似乎是李愛英變鉛青鏈黴菌dna異常修飾系統的分子生物學研究能表達的，因為它能紅補分別在dn劇、 dndb和咖jc發生單個基因突變的菌株，而且這種表達似乎也不會影響野生型菌株1326中原有的dnd基因簇的表達。
  19. S. lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation. it was speculated that dnda together with its downstream dna ( 2. 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64, while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s. lividans 1326

    另外通過比較這幾個突變株及野生型菌株在產孢和黑色素基因（ mel ）表達方面的差異，推測dnda及其下游區域與變鉛青鏈黴菌的產孢和刺激外源黑色素基因的表達有關，而dndb和dndc則與之無關，因為la1和la2在這兩種表型上與野生型菌株無明顯差異。
  20. Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation, development of the organisms, cell transformation and oxidative stress. till now, the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated, nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported. this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed, thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively. in this way, we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly, which will contribute a lot to further analysis

    鑒于plc - 1發揮上述作用的機制尚未完全闡明， plc - 1通路與其他信號通路間的交聯和代償尚無系統報道，又因為以往的研究方法不夠全面，本研究以野生型小鼠胚胎成纖維細胞（ plc - 1 ~ （ + / + ） ）和缺失磷脂酶c - 1的小鼠胚胎成纖維細胞（ plc - 1 ~ （ - / - ） ）為研究模型，在眾多蛋白組學策略中選擇了雙向電泳+質譜（ 2 - de + ms ）作為研究手段，通過對比表皮生長因子（ egf ）刺激24小時後上述兩種細胞的總蛋白質表達差異，全面地探討敲除plc - 1對生長因子誘導的信號傳遞的影響，從而間接研究plc - 1生物學作用、信號傳遞機制及其代償情況，為后續的深入研究打下基礎。
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