野酵母 的英文怎麼說
中文拼音 [yějiàomǔ]
野酵母
英文
wild yeast-
In order to study the mechanism of the effect of low concentration tfp on the proliferation of s. pombe, we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ). the fluorescence intensity reflected the cytosolic free calcium concentration. the result showed that, the cytosolic free ca2 + concentration in s. pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s. pombe cultured in medium containing 10umol / l ca24, while ca2 + concentration in s. pombe treated with 50umol / l tfp was 4 - 5 times higher
本文發現增加胞外鈣濃度以及低濃度（ 20 100 mol l ）三氟拉嗪（ tfp ）不但能促進野生型s . pombe細胞的增殖，而且對mfp7菌株也有同樣的效應，這說明胞外ca ~ （ 2 + ）和低濃度tfp對不同遺傳型裂殖酵母細胞的增殖均有促進作用。One agent is a wild yeast, which imparts a disagreeable bitterness to beer.
有一種致病菌是野酵母，使啤酒會有不適合的苦味。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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