金結合蛋白 的英文怎麼說
中文拼音 [jīnjiēgědànbái]
金結合蛋白
英文
metal-binding protein- 金 : Ⅰ名詞1 (金屬) metals 2 (錢) money 3 (古時金屬制的打擊樂器) ancient metal percussion instrum...
- 結 : 結動詞(長出果實或種子) bear (fruit); form (seed)
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 結合 : 1 (發生密切聯系; 聯合) combine; unite; integrate; link; binding; coalition; cohesion; connectio...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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By observing the effect of acidity on the uv difference spectra of ehpg or hbed, the spectral characteristics of apoovotransferrin binding to metals and anions at ph 7. 4 show that the reaction of apoovotransferrin with metals is related to deprotonation of the phenolic group of tyrosine of the metal binding sites, while the binding of apoovotransferrin to anions is associated with the protonation of the phenolic group of tyrosine of the anion binding sites
摘要通過觀察酸度對模擬轉鐵蛋白小分子ehpg和hbed紫外差光譜的影響,結合脫鐵伴清蛋白與金屬離子、陰離子結合的光譜特性,推斷脫鐵伴清蛋白與金屬離子的結合導致金屬離子結合位點處酪氨酸酚羥基的去質子化,而與陰離子的結合導致陰離子結合位點處酪氨酸酚羥基的質子化。In this paper, the ovarian development, oogenesis, vitellogenin molecular characteristic, vitellogenin synthesis and uptake, and vitellin degradation in nasonia vitripennis ( hymenoptera : pteromalidae ), are studied. the detailed results are shown as follows : 83 % of ovaries were found to be composed of eight ovarioles with four on each side and 17 % of ones to be composed of six ovarioles with three on each side. ovarioles appeared on the 10th day after parasitization, which were transpearant and had no egg chambers
為此,本論文就蠅類蛹期外寄生蜂? ?麗蠅蛹集金小蜂nasoniavitripennis (膜翅目:金小蜂科) ,卵巢發育、卵子發生,卵黃蛋白的分子特性,及卵黃原蛋白的合成、卵巢對卵黃原蛋白的攝取及卵子內卵黃磷蛋白的降解動態作了研究,結果如下:麗蠅蛹集金小蜂卵巢83由4對卵巢管組成, 17由3對卵巢管組成。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現,抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應;進而通過免疫金標結合電子顯微鏡觀察發現,大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上;統計結果表明,類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Study on competition reaction and replacement reaction of metallothioneins with lead ion
金屬硫蛋白結合鉛離子的競爭反應和置換反應研究By using circular dichroism spectroscopy method and ultraviolet absorption spectrophotometry, competition reaction and replacement reaction of metallothioneins with lead ion were investigated
Mt - 。採用圓二色性cd光譜法和紫外uv吸收光譜法研究金屬硫蛋白結合鉛離子的競爭反應和置換反應。Competition reaction shows that znpbmt - is formed while lead ion and zinc ion bind competitively to apomt - ; however, apomt - s metal binding sites are all took up by lead ion, which means apomt - is more sensitive to lead ion than apomt -
競爭結合apomt -形成的是金屬混配的金屬硫蛋白而apomt -的金屬結合位點則完全被pbThe results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明:麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白,分子量分別為181kd和136kd ; sds - page電泳分析:存在3條雌特異性帶,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向電泳結果顯示,在120kd附近有兩個特異性點,其等電點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root
首先,構建了ecbp21表達載體,誘導了重組蛋白的表達,並通過膠回收法獲得了大量純化重組ecbp21蛋白,制備了高效價、高特異性抗體;隨后,利用ecbp21抗體,結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究,結果顯示:在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域,而在細胞內未發現或僅有少量金顆粒分佈,表明ecbp21蛋白主要定位於細胞壁區域,這為細胞外cambp ( ecbp21 )的胞外存在提供了直接證據:進一步,利用ecbp21抗體,通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況,結果表明ecbp21在白芷各組織中均有分佈,但在葉、花、花序軸中分佈較多,而在葉柄、根中分佈較少。The study was undertaken to isolate fe ( ii ) - transporter cdna and related binding protein cdna under fe - deficiency stress from a fe - deficiency root cdna expression library of malus xiaojinensis by screening library using maize fe ( ii ) - transporter cdna and wheat tamre - bp cdna as a probe. the main results as follows : 1 out of approximately 120000 plaques, four positive cdna clones encoding fe ( ii ) - transporter proteins, designated pftl -
本試驗以蘋果屬小金海棠( malusxiaojinensischengetjiang )為試材,利用玉米fe ( )轉運蛋白基因片段以及小麥金屬反應元件結合蛋白tamre - bpcdna為探針,篩選小金海棠缺鐵根cdna表達文庫,目的在於克隆蘋果鐵高效基因型小金海棠的fe ( )轉運蛋白基因以及與缺鐵脅迫相關的結合蛋白基因。The results indicated that broom flower is 50 %, 80 % and 100 % higher in protein, fat and carbohydrate content respectively than the ten vegetables, and the amino acid composition is balanced and the content reaches 1875. 55mg / 100g, with 37. 92 % of essential amino acids. the trace element zinc and selenium, and vitamin b2 is significantly higher than the ten common vegetables
結果表明,金雀花的蛋白質、脂肪和碳水化合物的含量比10種常見蔬菜平均值分別高5濺, 8既和100 % ;氨基酸種類齊全,含量達1875 . 55mg / 1009 ,人體必需氨基酸占氨基酸總量的37 . 92 % ;微量元素鋅和硒,維生素b2等營養成分,明顯高於10種常見蔬菜。The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser ctcf, and kruppel - like zinc finger protein ; two members of the adams ( a disintegrin and metalloprotease domain ) family ; two members of integrin family ; several proteins involved in the signal transduction, cell - cycle control, chromatin remodeling and transcription repression ; and also some proteins of cell skeleton and some with unknown functions
鑒定出的蛋白包括多個與轉錄調控相關的鋅指蛋白,如鋅指蛋白198 、 263 、 14 、 224 、 zf6 、轉錄抑制因子ctcf樣蛋白、幻肚pple樣鋅指蛋白等:兩個含金屬蛋白酶結構域和整合素結合結構域的家族成員adam28和adam17 ;兩個整合素家族成員pz整合素和含十個egf樣結構域的整合素( tied ) ;與細胞信號轉導通路有關的蛋白;與細胞周期調控有關的蛋白;與染色體重塑和基因轉錄抑制有關的蛋白;細胞骨架蛋白以及其他功能未明的蛋白等。The very plasmid pbmb0474 was constructed. when the recombinant plasmid pbmb0474 was transferred into crystal negative bt strain bmb171 through electroporation, the expression of the fusion genes about 150kda - 160kda can be detected by sds - page. 4
將pbmb0474電轉化到bmb171中得到蘇雲金芽胞桿菌重組菌, sds - page結果顯示重組菌可以表達大小約150kda - 160kda的gfp融合蛋白。Part ii this paper has minutely studied the interaction between au ( iii ) and serum albumin. the binding of au ( iii ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 7. 43 + 0. 02 ). the scatchard analysis indicates that there exists several strong binding sites of au ( iii ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of au ( iii ) with hsa or bsa using uv - visible spectrometry at ph ( 7. 43 + 0. 02 ), which shows that the binding between au ( iii ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for au ( iii ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has project supported by the national science foundation of china ( no. 299621001 ), and the foundation for talents of the ten hunred thousand in guangxi
第二部分:生理ph ( 7 . 43 0 . 02 )條件下,用平衡透析法和紫外光譜,熒光光譜研究了金( )與人血清白蛋白( humanserumalbumin ,簡稱hsa )或牛血清白蛋白( bovineserumalbumin ,簡稱bsa )的結合。 scatchard圖分析表明,金( )在hsa或bsa中有強弱兩類結合部位,通過計算機擬合獲得結合的逐級穩定常數值。利用熒光猝滅法計算出金( ) - hsa和金( ) - bsa體系的stem - volmer常數和雙分子猝滅速率常數。The dynamics of cellular copper this study reealed also hae broad implications on the regulation of the metal ion content in metal - binding proteins
該研究所揭示的細胞內銅的動力學在金屬結合蛋白的金屬離子的調節上還具有更為廣泛的意義。The dynamics of cellular copper this study revealed also have broad implications on the regulation of the metal ion content in metal - binding proteins
該研究所揭示的細胞內銅的動力學在金屬結合蛋白的金屬離子的調節上還具有更為廣泛的意義。In this paper, rutilt oxide film, anatase oxide film on titanium, titanium surfaces containing calcium and containing phosphorus, and apatite coating - ti were surface characterized. based on investigation on apatite precipitation, protein adsorption and osteoblast culture in vitro on the titanium surfaces, the relationship between the bioactivity and the surface characterization of titanium was studied
在本論文中,通過金紅石二氧化鈦膜、銳鈦礦二氧化鈦膜、含鈣的鈦表面、含磷的鈦表面和ti -羥基磷灰石塗層的表徵,結合羥基磷灰石沉積、蛋白質吸附和細胞體外培養,研究了鈦的表面表徵與生物活性的關系。These mechanisms involve in the binding of heavy metals to cell wall, immobilization exclusion of the plasma membrane, efflux of these toxic metal ions, reduction of heavy metal transport, compartmentalization and metal chelation by tonoplast - located transporters, expression of more general stress response mechanisms such as stress proteins
這些機制包括重金屬離子與細胞壁的結合,限制重金屬離子透過細胞膜,重金屬離子或化合物的排出,重金屬在植物體內的轉移,重金屬的絡合與區隔化以及植物對脅迫的基本的反應機制如脅迫蛋白含量的變化等。The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future
為研究ndrgz的結構與功能,構建了ndrgz的酵母融合表達質粒,並在比赤酵母中得到可溶形式的表達;利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。In nature, the majority of metalloporphyrins are conjugated to proteins to form a number of very important molecules, such as the cytochromes
在自然界中,大多數金屬卟啉與蛋白質結合形成許多重要的分子,如細胞色素。A young trader in australian opals was very interested in master s design called " the hidden force connection ". he studied it repeatedly and praised master for her daring and elegant design. this delicate piece that accounted for the perfect combination of gold and opals really impressed him
和天飾同一館的廠家大部分是從事珠寶買賣,一位買賣澳大利亞蛋白石的年輕人,看到內邊力量溝通時,仔細欣賞多次,並表示師父大膽優雅的設計精細的手工,將蛋白石黃金,做出這么完美的結合,真令人激賞。分享友人