鏈式反應聚合 的英文怎麼說

中文拼音 [liànshìfǎnyīngjùgě]
鏈式反應聚合 英文
chain reaction polymerization
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 式 : 名詞1 （樣式） type; style 2 （格式） pattern; form 3 （儀式; 典禮） ceremony; ritual 4 （自然科...
  • 反 : Ⅰ名詞1 （方向相背） reverse side 2 （造反） rebellion 3 （指反革命、反動派） counterrevolutionari...
  • 應 : 應動詞1 （回答） answer; respond to; echo 2 （滿足要求） comply with; grant 3 （順應; 適應） suit...
  • 聚 : 動詞（聚集; 聚積） assemble; gather; get together
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 聚合 : 1 （聚集到一起） get together2 [化學] （單體結合成高分子化合物） polymerization; polymerize 3 [生...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Even minute amounts of dna can now be amplified, using the polymerase chain reaction, to provide sufficient material for genetic fingerprinting

    即使僅獲得很少量的dna也可使用聚合酶鏈式反應來擴增以提供足夠的材料進行遺傳指紋分析。
  3. Application of fluorescence assay method on rapidly detection of food

    套式逆轉錄聚合酶鏈反應在黃熱病毒快速檢測中的應用
  4. A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene

    以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。
  5. Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus

    傳染性造血器官壞死病毒逆轉錄聚合酶鏈式反應操作規程
  6. Polymers are most commonly formed by two main types of growth reaction traditionally referred to us condensation (step reaction) and addition (chain reaction) processes.

    形成聚合物的最普通方式是靠兩種主要的增長反應，習慣上分別稱做縮合（逐步反應）過程和加成（鏈式反應）過程。
  7. Detection of botulinum in food by pcr

    聚合酶鏈式反應對食物中肉毒的檢測
  8. Effects of process conditions on degradation of xanthan gum by - mannanase

    適合進行反轉錄聚合酶鏈式反應
  9. Founder mice will be examined by polymerase chain reaction ( pcr ) and southern blot

    採用聚合酶鏈式反應（ pcr ）和southern印跡對陽性鼠進行檢測。
  10. The series include : hcg test kit, afp test kit, cea test kit, em antibody test kit, as antibody test kit, etc. the series of pcr kits the series of pcr kits has very high sensitivity, excel distinctiveness and accurate results

    基因擴增檢測試劑系列是採用聚合酶鏈式反應技術研製而成，具有配套試劑全標本處理簡便靈敏度極高特異性強結果準確等優點，適用於醫院臨床檢測和科研。
  11. Standard guide for detection of nucleic acids of the mycobacterium tuberculosis complex and other pathogenic mycobacteria by the polymerase chain reaction technique

    用聚合酶鏈式反應方法探測結核分支桿菌復合物和其它病原分支桿菌的核酸的標準指南
  12. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  13. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。
  14. Polymerase chain reaction is a rapidly developing and widely used dna amplification technique, which is widely applied in life science and other related fields

    聚合酶鏈式反應（ polymerasechainreaction ，簡稱pcr ）技術是發展很快、應用很廣的體外擴增基因片斷技術，在生命科學研究及諸多相關領域已經得到了廣泛應用。
  15. The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template

    應用逆轉錄-聚合酶鏈式反應（ rt - pcr ）技術克隆得到編碼草魚生長激素（ cgh ）的基因cdna ，並定向克隆到puc18載體上。
  16. In this study, the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced. the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues

    研究採用反轉錄?聚合酶鏈式反應（ rt ? pcr ）技術對兩株分別從柳州（ gxlz ）和南寧（ gxnn ）分離的廣西流行豬瘟病毒（ classicalswinefevervirus ， csfv ）進行e _ 2全基因的擴增、克隆和測序。擴增片段長度為1090bp ，編碼364個氨基酸殘基。
  17. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  18. A pair of primers containing sph i and hind iii restriction sites were designed, according to the poifn - a gene in ddbj / genbank. then poifn - a gene was cloned from porcine genomic dna by pcr

    根據ddbj genbank基因庫中已登錄的豬干擾素基因序列，設計了含sph和hind酶切位點的一對引物，採取聚合酶鏈式反應（ pcr ）法，以豬基因組dna為模板進行了poifn克隆。
  19. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法（ pcr - rflp ）對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型，該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換，使蘇氨酸（ thr ， acc ）變為絲氨酸（ ser ， agc ） 。
  20. Strain pseudomonas psuedoalcaligenese ys1 was capable of producing phas containing monomer of hb and mcl has in certain medium. phacl and phac2, two key polyhydroxyalkanoates polymerase genes of pha biosynthesis were amplified and cloned from chromosomal dna of pseudomonas psuedoalcaligenese ys1 using pcr

    本研究利用聚合酶鏈式反應（ pcr ）技術，從p . psuedoalcaligeneseys1染色體dna中擴增並克隆了調控短鏈與中鏈pha生物合成的兩個關鍵酶基因： phac1 、 phac2基因。
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