鏈篩 的英文怎麼說

中文拼音 [liànshāi]
鏈篩 英文
chain grizzly
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 篩 : 名詞[書面語] （植物名） sedge
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. It can used to separate normal paraffin from branched hydrocarbon and cricoid hydrocarbons through selective adsorption

    5a分子篩吸附劑可通過選擇性吸附過程而從支鏈烴及環狀烴中分離出正構烷烴。
  5. Four c - ch3, six aromatic carbons, six carbons of mycosamine were the characteristics of candicidin d, one ketal corresponding to mycosamine and four ketons indicated that no hemiketal formed between c - 15 and c - 19 in fr - g08b or candicidin d. however, such hemiketal was usually thought having been formed

    用這個探針，對鏈黴菌fr - 008的基因文庫進行雜交篩選，獲得了3個陽性克隆，經southern雜交分析，發現它們均含有6 . 4kb共同的陽性片斷。
  6. Contrast between this machine and traditional rolling screen : adopt lower driving, the carrying ability is great, running is stable which settle the problem, such as great torsional moment of central drive, startup loading is great with shock of chain driving

    本機與老式滾筒篩相比，因為採用下傳動有承載能力大運轉平穩，特別克服了中心傳動扭矩巨大，起動負荷大，鏈傳動沖擊等缺點。
  7. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  8. Those mentioned above has already been proved either in and outside our country. infact, when the machine works on the track which is mudding and hardening, especially on hardening line, rm - 80 can not give full play to it ' s productivity, and sometime the machine can not go forward with excavating chain turnning, which results in a low productivity of not more than 100 meters per hour

    在我們的生產實際中，尤其在板結地段， rm80型全斷面道碴清篩機的作業效率就不能得以充分的發揮，經常出現挖掘鏈轉不動、清篩機不前進，或者是作業效率很低，以致於每小時作業速度不足百米的情況。
  9. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  10. Severity of the diseaseis closely related to temperaure, rh and nutrition of soils. copper - hydroxide, junduqing and streptomycin were the best controling efficacy in the tested bactericides screening in laboratory

    室內藥劑篩選試驗表明：可殺得、菌毒清、農用鏈黴素均有較好的抑制效果。
  11. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  12. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  13. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  14. Construction and screening of phage display single chain antibody library against bursaphelenchus xylophilus cellulase

    抗松材線蟲纖維素酶單鏈抗體庫的構建及篩選
  15. After the primary and secondary selection, 7 strains were tested to have higher productivity than the starting strain. of the 7 strains, strains s1 - 007, s1 - 123 and s1 - 018 were proved to produce more maduramicin than the starting strain. compared with 6236ug / ml of the starting strain, the productivity of the above three strains reached 8020 g / ml, 8254 g / mland9175 g / ml respectively

    出發菌株尤馬馬杜拉放線菌s - 1 ，經過鏈黴素抗性篩選，挑取223個菌株，經過初篩和復篩得到7株菌株馬杜黴素的產量高於出發菌株，其中3個菌株s1 - 007 、 s1 - 123和s1 - 018的產素量與出發菌株的6236ug ml相比有較大提高，分別為8020ug ml ， 8254ug ml和9175ug / ml ，尤其是突變菌株s1 - 018的產量比出發菌株高47 。
  16. Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies

    小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選
  17. The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications

    此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
  18. This result proved that uv treatment combined with tolerating apramycin or with tolerating streptomycin could be effective way to obtain high yield apramycin - producing strains

    證明了uv處理復合耐受自身產物、 uv處理復合耐受鏈黴素能獲得較好的篩選結果。
  19. It included low amylose and protein material ' s selection in early generation, low protein rice culture, purchase according to quality, paddy and brown rice choiceness and super low temperature store

    包括低直鏈澱粉及蛋白質含量材料的早期篩選，低蛋白米栽培，分品質收購，稻穀精選，糙米色選，超低溫貯藏技術等。
  20. The research work presented a large quantity of debris characteristics parameters, and especially made a thorough study on the characteristic description of sediment chain graph ; meanwhile, the sensibility, differentiation and information redundance analyses of the characteristic parameters also supplies the quantitative indexes for the filtration and optimization of the debris characteristic parameters ; in addition, the debris fusion decision recognition method based on the proof fusion theory and the comprehensive debris recognition flow provide reliable recognition arithmetic for debris recognition ; and lastly, the fault fusion diagnosis judging method based on ferro - graph and spectral analysis provides the basic diagnosis method in theory for multi - fault premonitory diagnosis system of aero - engine

    本文研究工作提出了大量磨粒特徵參數，尤其深入研究了沉積鏈譜片的特徵描述問題；同時，特徵參數的敏感性、區分度與冗餘度分析為磨粒特徵參數的篩選優化提供了量化指標；另外，基於證據融合理論的磨粒統計融合決策識別方法以及磨粒綜合識別流程為磨粒識別提供了可靠的識別演算法；最後，基於光譜和鐵譜信息的磨損故障融合診斷決策方法為發動機多故障徵兆綜合診斷系統提供了基本的診斷理論手段。
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