鏈黴菌素 的英文怎麼說

中文拼音 [liànméijūnsù]
鏈黴菌素 英文
streptin
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 黴菌 : [微生物學] mould; mycete; mucedine
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. Avermectins are a series of 16 - membered macrocyclic lactones produced by streptomyces avermitilis with potent anthelmintic and insecticidal activity. they are the most effective agricultural pesticides and antiparasitic agents, and used widely in medical, veterinary and agricultural fields

    阿維鏈黴菌（ streptomycesavermitilis ）產生的阿維菌素（ avermectins ）是一組高效、低毒、廣譜的殺蟲抗生素，在醫藥、農業和畜牧業生產中具有良好的應用價值和廣闊的市場前景。
  3. Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted

    因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。
  4. All of our products is exported likelactobacillus acidophilus, bidifobacterium longum, lactobacillus paracasei, lactobacillus rhamnosus, tagatose, lnulin, nisin, natamycin, - polylysine, nucleotides mix, adenosine monophosphate, cytidine monophosphate, guanosine monophosphate disodium, uridine monophosphate disodium, calcium inosinate, calcium guanylate, calcium 5 - ribonucleotide, damp, dcmp, dgmp, tmp, deoxyadenosine, 2, 6 - diaminopurine nucleoside, 2, 6 - diaminopurine deoxynucleoside, adenine arabinoside, adenosine triphosphate, uridine triphosphate, guanosine triphosphate, cytidine triphosphate, cytidine diphosphate choline （ citicoline ） to all over the world, if you are interested in our products, please do visit our website, hope there will fullfill your requirement

    主營產品有益生菌（嗜酸乳桿菌、長雙歧桿菌、乾酪乳桿菌、鼠李糖乳桿菌） 、塔格糖、菊粉（菊糖） 、乳酸鏈球菌素、納他黴素、 -聚賴氨酸、核苷酸，腺苷酸，胞苷酸，鳥苷酸二鈉，尿苷酸二鈉，肌苷酸，鳥苷酸鈣，核糖核苷酸鈣，脫氧腺苷酸，脫氧胞苷酸，胸苷酸二鈉，脫氧鳥苷酸二鈉，脫氧腺苷， 2 -氨基脫氧腺苷， 2 -氨基腺苷，阿糖腺苷，胞二磷膽堿，腺苷三磷酸，三磷酸尿苷，三磷酸胞苷，三磷酸鳥苷等
  5. Effects of cultivation conditions on natamycin production by streptomyces gilvosporeus

    培養條件對褐黃孢鏈黴菌發酵合成納他黴素的影響
  6. So two of them maybe belong to a new genus. according to the results of polyphasic taxonomy, the phylogenetic relationship of thirteen strains were defied that seven strains such as 40001 belong to streptomyces, strain 40009 belong to nocardiopsis, strain 40010 and 40011 belong to micromonospora, strain 40012 belong to streptosporangium ; strain 40007 and 40008 have similar relationship with actinomadura, but their sequence similarity are lower than 97 %, may belong to a new genus

    實驗獲得了13株放線菌16srdna的近乎全序列，將之與genbank中已知序列進行比較，也可以將這13株放線菌劃分為5個類群，菌株40001等7株菌與鏈黴菌屬（ streptomyces ）的菌株進化關系較近，其中菌株40004和波賽鏈黴菌（ streptomycespeucetius ）的序列相似性為98 . 03 ； 40013與拒黴素鏈黴菌（ streptomycesresistomycificus ）的序列相似性為99 . 02 。
  7. Fr - 008 is a strain producing a complex of heptaene macrolide antifungal antibiotics, one of which, previously named fr - 008 was regarded to bis similar to candicidin, but different in sugar moiety

    Hplc分析研究表明，它與灰色鏈黴菌imru3570產生的殺假絲菌素是同一種物質，均含有相同的幾種主要組分。
  8. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  9. First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d

    首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。
  10. But in s. mycarofaciens, there is no distinctive propionate kinase, so that the utilization rate of propionic acid is lower than that of acetic acid. for this reason, it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s. mycarofaciens and s. erythreus, which would transfer propionate kinase of s. erythreus to s. mycarofaciens. therefore, the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ), which would reduce the production costs

    根據文獻報道，紅黴素鏈黴菌中的丙酸激酶對丙酸的利用率是對乙酸的利用率的13倍；而在生米卡鏈黴菌中，無特異的丙酸激酶，菌體對丙酸的利用低於對乙酸的利用，因此希望利用原生質體種間融合的方法將紅黴素鏈黴菌的丙酸激酶基因轉移到生米卡鏈黴菌中，從而使融合子能夠利用丙酸鹽作為合成mdma _ 1的前體，提高mdma _ 1的產量，降低生產成本。
  11. 464 and erythromycin producer s. erythreus as original strains, studies were carried out for researching higher producers and enhancing content of midecamycin ( mdm ) ai, which could use propionate as precursor to reduce production costs. according to the literature, the utilization rate of propionic acid is thirteen times to that of acetic acid by propionate kinase in s. erythreus

    本論文主要研究了生米卡鏈黴菌與紅黴素鏈黴菌原生質體種間融合育種，以提高麥迪黴素a _ 1 （ mdma _ 1 ）的組分，增加其對丙酸鹽的利用度，降低生產成本。
  12. In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts

    我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。
  13. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  14. The system of ion beam implantation had been established and the biological effects were primarily studied

    壯觀鏈黴菌1043為一壯觀黴素生產菌種，通過實驗建立了離子注入選育壯觀鏈黴菌。
  15. S. lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation. it was speculated that dnda together with its downstream dna ( 2. 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64, while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s. lividans 1326

    另外通過比較這幾個突變株及野生型菌株在產孢和黑色素基因（ mel ）表達方面的差異，推測dnda及其下游區域與變鉛青鏈黴菌的產孢和刺激外源黑色素基因的表達有關，而dndb和dndc則與之無關，因為la1和la2在這兩種表型上與野生型菌株無明顯差異。
  16. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  17. To study the biosynthetics genes of apramycin in streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly

    為了研究黑暗鏈黴菌安普黴素生物合成基因，首先通過鳥槍克隆的方法克隆其安普黴素抗性基因。
  18. Also the ci represser was expressed, presumably from its own phage promoter and prevented transcription from pr at low temperature. moreover, s. lividans strains expressing the c. 100 kda pks were different in sporulation and antibiotic production compared to strains without the 2. 7 kb pks gene, suggesting that the pks protein was active in an unknown manner in streptomyces

    表達pks的變鉛青鏈黴菌菌株在孢子形成和抗生素產生方面與沒有2 . 7kbpks基因的菌株相比較是有區別的，因而推測在鏈黴菌中表達的雙功能結構域pks蛋白具有活性。
  19. The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4. 0 and 11. 5. the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state. when streptomyces griseus atcc14811 was cultured in 10. 3 % sucrose yeme liquid medium, production of extracellular cholesterol oxidase increased for 5 days before decrease

    利用硫酸銨鹽析及deae -纖維素離子交換柱層析提取純化灰色鏈黴菌atcc14811發酵上清液中的膽固醇氧化酶，理化性質研究表明酶作用晟適ph為8 . 0 ，最適溫度為45 ， ph穩定范圍在ph4 . 0 - 11 . 5之間，在50條件下保溫4h ，仍保留54酶活力。
  20. Using colophony - paraffin ( cp ) embedding tissue section technique and immunohistochemical streptalidin - peroxidase ( sp ) method, we have investigated the distribution of 5 - ht neurons in the brain of camponotus japonicus ( big worker ant ), compared the immunoreactivity with gaba, and primarily discussed the character of 5 - ht distribution. the results show that 5 - ht immunoreactive processes originate from a relatively small number of cell bodies but each neuron has processes over a large volume of the neuropil of the brain

    本實驗採用樹脂石蠟（ cp ）組織包埋切片技術和鏈黴菌抗生物素蛋白-過氧化物酶（ sp ）免疫組織化學方法，研究了5 - ht能神經元在日本弓背蟻（ camponotusjaponicus ）大工蟻腦中的分佈情況，並與gaba的分佈進行了比較，初步探討了5 - ht在各個腦區的分佈規律和特點。
分享友人
分享到 Line

分享到臉書