鑒別培養基 的英文怎麼說
中文拼音 [jiànbiépéiyǎngjī]
鑒別培養基
英文
differential medium-
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。Based on the constructivism theory, following the suggestions and requests in the fully time compulsory education curriculum standards of chemistry ( experiment draft ), this thesis makes it clear that the experimental inquiry and the investigating inquiry are the main approaches in the inquiry learning activities. after analyzing these two approaches in detail, the author proposes some concrete learning in scientific inquiry activities according to the characteristics of these two inquiry approaches. they are inquiry experiments, observation, designing the experiment, deducing, differentiating and measuring which are based on the experiments ; visiting, reading, collecting materials, asking and investigation which are based on the investigation
本文主要以建構主義理論為依據，以《標準》中對探究學習活動的建議和要求出發，明確了實驗探究和調查探究是開展探究學習活動的主要方式，以及兩種探究學習方式的特點；並深入分析了實驗探究和調查探究，提出了化學教學中具體的探究學習方式，分別為以實驗為基礎的探究實驗、觀察、設計實驗、推斷、鑒別、測定和以調查為基礎的參觀、查閱、收集及調查，使化學教學中對學生進行探究學習培養具有針對性。In this thesis the lbsp identification medium screening techniques was used. only after one cycle, large quantities no - function recombination plamid and enzyme activity increasing 20 times recombination plasmid were obtained
本研究用lbsp鑒別培養基直接篩選技術，僅一個循環就獲得了大量無酶活的重組質粒和酶活提高了20倍的突變基因。Observation on the conidia of 6 isolates belonging to four genera by sem indicated that there were remarkable difference between the genera. it was confirmed that the numbers of appendages and cells of conidia were steady characters for identification in the genera and species level. and the appendage knobbed on the top was also a very important character for the species identification
對以上四屬11個種單孢分離菌株在pda培養基上培養性狀的觀察表明，菌落形態和顏色、菌落背面顏色和輪紋、產孢時間和產孢類型在屬間具有重要的鑒別意義，在種內雖然不穩定，但不同種間確實存在差異，可以作為種間劃分的參考依據。分享友人