鑒定培養基 的英文怎麼說

中文拼音 [jiàndìngpéiyǎngjī]
鑒定培養基 英文
differential medium
  • 鑒 : Ⅰ名詞1 （鏡子 古代用銅製成） ancient bronze mirror2 （可以作為警戒或引為教訓的事） warning; objec...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 鑒定 : 1 （評語） appraisal （of a person s strong and weak points） 2 （評定） appraise; identify; auth...
  1. We selected the most adaptive culture medium, temperature and light to produce abundant and natural conidia. we also studied the formation of distosepta. conidia germination, the characters of the formation of conidia on the conidiophores

    分離鑒定的同時，注意對適宜培養基、培養條件，分生孢子的分隔、萌發、產孢方式等特性進行觀察、探索和總結，最終總結出相對簡便易行的屬下種級分類標準。
  2. Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied

    植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定，通過初篩和復篩，得到一株產植酸酶較高的黑麴黴（ aspergillusniger ） an00101菌株。
  3. The results showed that lamellae were fit tissue for mycelia isolation, and success percentages of mycelia isolation with media pdas, pdaw, bm, pda are 74. 4 %, 35. 5 %, 15. 6 % and 8. 9 % respectively. the results also showed that stipe, mycorrhizae, and soil with the fungi are not fit for tricholoma matsutake isolation

    另外，馬鈴薯葡萄糖麥數濾汁培養基（ pdaw ） 、 bm培養基、 pda培養基也從菌褶2曾東方博士論文:腐生與共生食用菌菌絲體分離、培養及其dna鑒定研究分離到松茸菌絲體，分離成功率依次是35 . 5 % 、巧
  4. Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min

    結論1 、以從自然界中篩選出的菌株為出發株，經誘變、篩選，得一高產葡甘聚糖酶菌株，初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養，該菌株的最適培養條件：培養基ph值7 . 0 ，碳源2 . 5 ，振蕩培養， 200r min ，培養溫度36 ，培養48h 。
  5. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  6. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  7. First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d

    首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。
  8. Isolation, culture and identification of human adipose tissue derived stem cells

    人脂肪組織分離培養基質幹細胞的方法及其表型鑒定
  9. After 30 days. green buds were rooted on 1 / 2 strength mso medium containing lomg / l km. plantlet with normal roots were transferred to field for further analysis. 3

    30天後可分化出綠芽，把分化出的綠芽在1 / 2ms0 + 10mg / lkm培養基上進行生根篩選，把能夠正常生根的苗移栽田間，做進一步的鑒定。
  10. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  11. Enriched culture medium

    微生物鑒定培養基
  12. Its " optimum concentration of trypan blue was from 0. 005 % to 0. 01 % ( w / v ), the optimum volume was around 15ml / per petri dish ( 080mm ). lbsp medium was easy to use and can be sterilized by 121 擄 c for 30 minutes

    比較研究結果表明鑒定培養基的適宜條件是：錐蟲藍的使用濃度是0 . 005 - 0 . 01 （ w v ） ，培養基的用量為15ml皿左右，並可以採用0 . 1mpa高壓蒸汽滅菌。
  13. Their morphological and physiological characteristics were observed through the strains colony morphology, size, color, growth rate, texture, and spores

    用察氏平板培養基分離菌株，根據菌株的菌落形態、大小、顏色、生長速率、質地、生長培養基顏色變化以及菌絲體和抱子的形態特徵進行鑒定。
  14. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  15. 2. the resistence of transformators were selected by g418 after co - culture with agrobactrium tumeflien, which was 40 mg / l in subcultured medium and 50mg / l in differeniated medium. in the process of killing agrobactrium, cef, amp or carb were also useful and the time of inhibition was long too

    經與農桿菌共培養后轉化子的鑒定選用g418 ，在繼代篩選培養基中添加40mg l ，在分化篩選培養基中添加50mg l ；在除菌過程中，頭孢噻肟鈉、羧芐青霉素、氨芐青霉素抑菌效果較好，抑菌時間較長。
  16. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  17. In contrast, there was very little difference in gene expression between calli subcultured for one week and two weeks with only 0. 78 % of the genes showing significant different expression. ( 4 ) the 14 genes with different expression level between the control and the transformed sublines a78 - 3 and a78 - 4 were classified by their function

    88的未鑒定功能基因的表達呈顯著差異；而在愈傷組織培養二周與愈傷組織培養一周之間，則在第大類、第v大類和第大類未鑒定基因中各有1個表達呈顯著差異。
  18. Observation on the conidia of 6 isolates belonging to four genera by sem indicated that there were remarkable difference between the genera. it was confirmed that the numbers of appendages and cells of conidia were steady characters for identification in the genera and species level. and the appendage knobbed on the top was also a very important character for the species identification

    對以上四屬11個種單孢分離菌株在pda培養基上培養性狀的觀察表明，菌落形態和顏色、菌落背面顏色和輪紋、產孢時間和產孢類型在屬間具有重要的鑒別意義，在種內雖然不穩定，但不同種間確實存在差異，可以作為種間劃分的參考依據。
  19. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  20. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
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