阻遏基因 的英文怎麼說
中文拼音 [zǔèjīyīn]
阻遏基因
英文
repressor gene-
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。With the rise and fall of the hegemon based on un - equilibrium development in its power, the hegemonic system also developed cyclically. because the hegemonic prestige can offer great profits, it is the most important national interest for hegemon, and therefore, to keep and preserve its hegemonic prestige through averting the adversaries or potential adversaries will be the key of hegemon ' s foreign policy
霸權體系可以為霸權國家帶來巨大的國際收益，維護霸權地位因而成為霸權國家的最高國家利益，霸權體系中的大國及區域性大國因其對霸權國家霸權地位的潛在威脅而成為霸權國家防範和遏制的重點對象，削弱這些國家的權力基礎、延阻它們的權力增長是霸權國家霸權護持戰略的核心。As both the recombinant plasmids and the host dh5a had no laciq gene which encode lac represser, the trc promoter was not repressed by lac represser and always active. therefore the afpga could be constitutively expressed without iptg induction in the host dh5. in the shaking flask, the optimum ph and temperature for afpga expression was 7. 0 and 30
兩重組質粒再轉化宿主菌dh5 ，由於重組質粒和宿主菌dh5均不含laci ~ q調控基因，重組菌不產生阻遏蛋白，控製表達的trc啟動子始終處于「開」狀態，因而所得重組菌dh5 pkkfpga和dh5 psmlfpga不需誘導便能組成型表達pga 。
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