限菌培養 的英文怎麼說
中文拼音 [xiànjūnpéiyǎng]
限菌培養
英文
gnotobiotic culture-
The biological characteristics of mycelia from phellinus igniarius and culture media were studied. two kinds of culture media were suitable for the growth of mycelia. the result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia, and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation
為了最大限度地保存菌種的活力，以提高菌絲體的質量及菌絲體內活性成分的累積，本文通過對比研究，進一步對其生長基質進行篩選，明確了兩種適于桑黃菌絲生長的固體培養基：以馬鈴薯為氮源、蔗糖為碳源的培養基較適用於菌絲收集，以蛋白腖為氮源、可溶性澱粉為碳源的培養基較適用於菌種的保藏。The results suggested that l. acidophilus pblgrew well with enterococcus strains ml, pb2, a30, a31. and had effective inhibition on the pathogens, the best combination were l. acidophilus pb1 with enterococcus m1, a30. l. acidophilus a878 grew not well with enterococcus strains ml, pb2, a30, a31. the growth inhibition of enterpathogens was lower than l. acidophilus pb1
在pb1與各球菌混合培養的基礎上，再和inf和pba混合培養，結果發酵液中inf和pba的生長受到限制，結果表明，在體外pba和inf很難與需氧菌共生。Briefly, the method includes four steps : culturing a sample to the maximum growth of autochthonous microorganism, filtering to remove the original biomass, inoculating the filtrate with certain kind of nitrogen fixing bacterium and determining the bacterial growth potential
該方法主要包括4個步驟：將樣本在合適的條件下培養，讓土著微生物得到最大限度的生長，然後過濾或離心去除初級生物量，在濾液中接入固氮細菌，並測定固氮細菌的生長潛能。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。The predicted protein contained 441 amino acids. the activity of the epsp synthase encoded by these two subclones was shown by complementation of the aroa mutation in e. coli strain er2799, indicating two subclones possess a intact activity of the enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase
採用互補試驗驗證分離的與草甘膦耐受相關的dna片段的生物學功能，結果顯示r3h1和r7h1均能使大腸桿菌epsp缺陷型菌株er2799恢復在限制性培養基上的生長能力，證明這兩個亞克隆的插入片段具有完整的epsp合成酶功能。For revealing the mechanism of low bacterial biomass in the lake, limiting factors for bacterial growth were studied with pure cultural and natural cultural tests
為了探明西湖水體中細菌生物量低的原因，通過添加不同營養鹽類的純種和自然培養試驗，對異養細菌生長的限制因子進行了研究。There is no acrystalliferous derivative was screened after treating 4. 5 g / ml ethidium bromide, but after elevating growth temperature of ybt - 1463 and other 8 bacillus thuringiensis parental strains to 42, 9 acrystalliferous derivative were obtained. a series of partially plasmid - cured derivatives were further obtained from 3 acrystalliferous derivatives originated from wild - type strain ybt - 1463 by elevating temperature to 44
用限量培養基和42培養9株野生菌株，結果篩選到9株cry ~ -突變株；繼續升溫至44來培養其中的三個cry ~ -突變株，得到內生質粒被進一步消除的突變株。However, the content of coq10 in wild strains of psb is limited after all. through construction of recombinant strain producing coq10 and optimization of the culture medium and fermentation conditions of a selected strain, the content of coq10 in psb will be increased markedly. it can not only remarkably strengthen the effect of psb feed additive, but also lay foundation for the exploitation of coq10
但野生型psb菌株coq _ （ 10 ）含量終究有限，如能選擇合適菌株進行培養優化，並通過構建基因工程菌，使菌體中coq _ （ 10 ）的含量成倍提高，則不僅可顯著增強psb飼料添加劑的應用效果，更重要的是，為coq _ （ 10 ）相關產品的研發奠定良好的基礎。分享友人