限速酶 的英文怎麼說
中文拼音 [xiànsù]
限速酶
英文
rate-limiting enzyme-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step
Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin, related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied. for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work
利用現代分子生物學和基因工程技術手段，克隆青蒿素生成途徑的關鍵酶基因，研究關鍵酶基因對青蒿素生物合成的調控規律，是打破青蒿素生物合成的限速步驟，大幅度提高青蒿素含量，最終達到利用植物生物技術工業化生產青蒿素的目的必須解決的關鍵問題。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Sinensis and e. j. hepuensis has been found in the sequences of the portions of 16s rdna and pcr / rflp studies of 110 samples, from six river valleys in eastern mainland of china. these subspecies - specific restriction sites allow rapid discrimination with the endonuclease dra i, and therefore can be used as a diagnostic genetic marker for identification of the two subspecies
通過對中國大陸東部6個水系110個絨螯蟹個體16srdna部分序列的測定和pcr rflp分析，發現在合浦絨螯蟹與中華絨螯蟹之間存在3 4個固定的堿基替代，這種亞摘要種特異性的限制性位點可以通過限制性內切酶dra進行快速檢測，成為2個亞種的分子鑒定標記。Acc synthase is the rate - limiting enzyme of ethylene biosynthesis, and the rate of ethylene biosynthesis is tightly controlled by the activeness of acc synthase in plants
Acc合酶（ acs ）是高等植物乙烯生物合成途徑中的限速酶，它在植物組織內的活性大小往往決定著乙烯產生的速率。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環， n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶，也是整個反應體系的限速酶。Prostaglandin e synthase ( pges ) is the terminal rate - limiting enzyme for pge2 synthesis, which catalyzes the conversion of pgh2 into pge2. there are two isoforms of pges, microsomal pges ( mpges ) and cytosolic pges ( cpges )
前列腺素e合成酶（ pges ）是pge _ 2生物合成過程中的終端限速酶，催化環氧合酶的產物pgh _ 2轉化為pge _ 2 。Prostaglandin e synthase ( pges ) is the terminal rate - limiting enzyme for pge2 synthesis, which catalyzes the conversion of pgh2 into pge2
前列腺素e合成酶（ prostaglandinesynthase ， pges ）是pge2生物合成過程的末端限速酶，催化環氧合酶的產物pgh2轉化成pge2 。Packaging pills with their own bodyguards ( in this case, molecules called protease inhibitors ) could enable protein - based drugs to survive, but it would not aid them in crossing the gut lining ; they are too big to slip into the blood as easily as more typical drugs, which generally consist of small molecules
為藥物配備保鏢（上述例子的保鏢分子稱為蛋白酶抑制劑） ，雖然可以讓蛋白質藥物保存下來，但卻無法幫助藥物穿過腸壁，因為這些分子太大，無法像一般小分子藥物那般輕易進入血液，而且包覆層對藥物動力學（藥物進入血液的速率以及停留在身體組織與器官的時間）的控制能力也相當有限。Effects of conjugated linoleic acid on the expression of critical enzymes of linoleic acid metabolism in tumor cell
共軛亞油酸對腫瘤細胞亞油酸代謝途徑中限速酶的影響分享友人