陽性組分 的英文怎麼說

中文拼音 [yángxìngzǔfēn]
陽性組分 英文
positive component
  • 陽 : Ⅰ名詞1 （太陽; 日光） the sun 2 （山的南面; 水的北面） south of a hill or north of a river 3 （中...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 陽性 : 1. [醫學] positive 2. [語言學] （與陰性相對的） masculine gender
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. P - liquid scintillation counting was used for analysis of erk and immunocytochemistry was used for the analysis of c - jun. all the data were analyzed with statistical treatment. the results were showed as follows : 1

    分別做p液閃計數觀察erk的活性表達，做westernblot觀察jnk的表達量變化，免疫組化觀察cjun陽性信號的表達，並進行統計學處理，結果如下： 1
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  5. Methods the optimal proportion of levant storax oil and dalbergiae odoriferae oil, which were active ingredients of the traditional chinese medicine styrax and dalbergiae odoriferae, was bolted by using uniform - design method ; the myocardial ischemia model of rats was set up through the induction of isoprel and the above rats were divided into 5 groups, which were model control group, high dose group of shuangxiangyou, middle dose group of shuangxiangyou, low dose group of shuangxiangyou, and positive control group of muskone

    方法傳統中藥蘇合香和降香的有效成分蘇合香油和降香油最佳配比採用均勻設計方法篩選；建立由異丙腎上腺素造成的心肌缺血大鼠模型，將實驗分為模型對照組、雙香油高、中、低3個劑量組及冠心蘇合丸陽性藥物對照組后進行藥效學實驗。
  6. Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages

    然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。
  7. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  8. Injected group, 0. 1 % saccharin ( 1. 5 ~ 2ml / rat, in 5min ) intraoral infused group and cta group. the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ), lateral part of mediodorsal thalamic nucleus ( mdl ), ventroposterolateral thalamic nucleus ( vpl ), ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry. in behavioral experiment, 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i. p

    為探討cta形成過程中enk的作用，本實驗用成年雄性sd大鼠35隻，分為空白對照組、生理鹽水（ 2體重）腹腔注射組、 0 . 15mlicl溶液（ 2體重）腹腔注射組、 0 . 1糖精溶液口腔灌流組（ 1 . 5 - 2ml只， 5min ）和cta建立組，採用免疫細胞化學方法，觀察了亮腦啡肽（ lek ）陽性神經元在大鼠腦內的分佈情況，並比較了各組大鼠丘腦外側背核（ ld ） 、丘腦內側背核外側部（ mdl ） 、丘腦腹后外側核（ vpl ） 、丘腦腹后內側核（ vpm ）以及丘腦網狀核（ rt ）等5個腦區內lek表達水平的差異；另外將成年雄性sd大鼠18隻，分為正常cta建立組以及在cta建立前或cta建立后阿片受體拮抗劑納洛酮（ 2mg kg體重）腹腔注射組，對內源性阿片樣物質對于cta建立和保持的影響進行了行為學研究。
  9. Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased

    腦內gfap陽性結構也明顯增多，其分佈與fos陽性細胞分佈基本一致，表現為胞體肥大、突起粗長； ast緊密包繞在神經元周圍形成神經元- ast復合體（ n - asc ） ；在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ； （ 2 ）免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區（ edas ） ，高滲刺激后數量明顯增多： （ 3 ）將們個mp注入大鼠一側n卜、卜卜或孤束核（ ws ） ，分別在延髓內臟帶（ mvz ） 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ； （ 4 ）高滲刺激使培養神經元和ast內鈣水平先升高后降低，最後維持在比高滲刺激前稍高的靜息鈣水平上。
  10. The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos

    本實驗採用免疫組織化學技術，應用13種不同的抗血清，對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較，結果如下：揚子鱷胚胎胰腺中，生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天； p物質免疫陽性細胞出現于第18天； p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。
  11. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  12. Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in

    以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。
  13. Methods : the methods of the increase of blood capillary permeability of the abdominal in mice induced by injection of acetic acid and the swelling of the ear in mice caused by crotin were used for anti - inflammatory experiments, aspirin and hydrocortison were respectively used as the positive control ; the methods of abdominal pain in mice induced by acetic acid and woolfe - macdonald were used for analgesic experiments, morphine hydrochloride as the positive control

    方法：小鼠隨機分組，抗炎實驗用醋酸誘發小鼠腹腔毛細血管通透性增高和用巴豆油誘發小鼠耳腫脹，陽性對照分別為阿斯匹林組和氫化可的松組；鎮痛實驗採用醋酸誘發小鼠腹痛和用熱板誘發小鼠足痛，陽性對照為鹽酸嗎啡組。
  14. Results in the lung cancer group, the positive rate of telomerase activity in surgically resected lung cancer tissues was 91. 7 ( 33 out of 36 samples ), and 85. 7 ( 6 out of 7 samples ) and 71. 4 ( 5 out of 7 samples ) in fiberobronchoscopically collected tissues or cells and pleural effusion cells, respectively

    結果肺癌組： 36例手術切除肺癌組織中端粒酶陽性率為91 . 7 ， 7例纖維支氣管鏡活檢肺癌組織和7例癌性胸水細胞中端粒酶陽性例數分別為6例和5例；總檢出率為88 （ 44 50 ） 。
  15. Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high

    結果表明： （ 1 ）脂質體包裹外源基因轉染精子的方法，可將外源基因導入受精卵中，能夠獲得轉基因動物，並得到了較高的轉基因陽性率； （ 2 ）精子攜帶的外源dna的整合過程是隨機的，在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。
  16. Quorum - sensing systems can be divided into two paradigmatic classes : luxi / luxr - type quorum - sensing systems in gram - negative bacteria which produce acyl - homoserine lactone ( ahl ) autoinducers, and oligopeptide / two - component - type quorum - sensing ciruits in gram - positive bacteria which produce autoinducing peptide ( aip ) autoinducers. in contrast to the two paradigmatic quorum - sensing systems, vibrio harveyi produces and responds to an ahl - type autoinducer termed ai - 1 and a novel signal molecule named ai - 2

    Qs系統可分為兩種典型的類型，即革蘭氏陰性細菌的luxi luxr型qs系統，革蘭氏陽性細菌的寡肽雙組分型qs系統，分別以酰基高絲氨酸內酯（ acyl - homoserinelactones ， ahl ） 、寡肽（ autoinducingpeptide ， aip ）為信號分子。
  17. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  18. Four hyg - resistant plants, which were chosen randomly from the plants which grown in the field, were detected through gus and pcr. the results confirmed that the npr1 gene have been induced into the genome of eustoma russellianum

    隨機選取4株移栽成活的具有hyg抗性的洋桔梗植株進行gus染色和pcr分子檢測，均為陽性，初步證明外源npr1基因已整合到洋桔梗基因組中。
  19. Methods impedance pneumoplethysmogram ( ipg ) and impedance respirogram ( irg ) were detected in 64 cases of patients with phd, and investigate dmf rheogram positive rate, also analysed various parameters of ipg in positive and negative groups

    方法對64例肺心病患者進行呼吸阻抗圖和肺阻抗圖檢測，分組統計膈肌疲勞圖形陽性率；並對陽性組和陰性組進行肺阻抗圖各參數比較。
  20. Results ( 1 ) the positive rate of dmf. rheogram in phd with and without respiratory failure are 75 and 21. 9 respectively. ( 2 ) the positve rate in phd with and without heart failue are 80 and 46. 3 respectively. ( 3 ) the mean values of various parameters of ipg in positive and negative dmf rheogram have no significant difference. conclusion respiratory failure and heart falure in phd are closely correlated with dmf

    結果（ 1 ）肺心病伴與不伴呼衰組膈肌疲勞圖形陽性率分別為75與21 . 9 ； （ 2 ）肺心病伴與不伴心衰組膈肌疲勞圖形陽性率分別為80與46 . 3 ； （ 3 ）膈肌疲勞陽性組與陰性組肺阻抗圖各參數均值無顯著性差異。
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