隆內高 的英文怎麼說
中文拼音 [lōngnèigāo]
隆內高
英文
lugnegard-
Cloning and expression of a new acyl - homoserine lactone hydrolase gene
一種新的乙酰高絲氨酸內酯水解酶基因的克隆和表達The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Kuantien is easily accessible via provincial highway 1, lungtien railway station or the sun yat - sen freeway, making travel to and from the town hassle - free. local tourist attractions include wushantou reservoir, the national tainan institute for the arts, artifacts from the prehistoric black pottery culture, the lungtien distillery, and the wildlife preserve for the endangered pheasant - tailed jacana. but the most indelible image of a visit is the sight of people rafting through the water caltrop ponds in tungchuang and hsichuang villages
四通八達的官田鄉有臺一省道鐵路縱貫縣隆田站中山高速公路貫穿其間,往返順暢,鄉內更有烏山頭水庫國立臺南藝術學院黑陶文化遺址隆田酒廠葫蘆埤水雉保護區等聞名的風景區,其中尤以東西莊地區的菱香舟影,名列新南瀛勝景之一。Lungtien s visitor center offers an introduction to distilling. local products flavored with lung feng liquor such as popsicles and luwei, yushan sausages made with kaoliang, and eggs hard - boiled in shaoxing rice wine, are also available
隆田酒廠的展示中心內展示了制酒過程,現場並可選購鳳雞爪酒冰棒以高粱酒製作的玉山臘味香腸和紹興不老蛋等。It was dark, but at eleven o clock the moon rose in the midst of the ocean, whose every wave she silvered, and then, " ascending high, " played in floods of pale light on the rocky hills of this second pelion. the island was familiar to the crew of the young amelia, - it was one of her regular haunts. as to dant
天很黑,但到了十一點鐘,月亮從海上升了起來,把海面上染成了一片銀色,然後,又一步步上升,把蒼白色的光瀉滿了這座堪稱皮隆此山為希臘東北境內的高山,山中林木茂盛,景色秀麗,在希臘神話詩等文學記載中十分著名。But today, because of the factors of open marketing prime cost, transparent technology, similar or “ cloning ” marketing approaches, the domestic construction machinery producing enterprises not only can ’ t attain their goals solely depending on the price war, but also suffered a great impact on that. under this circumstance, what kinds of factors determining the success of one enterprise ’ s marketing strategy is the common focus from various enterprises
但由於當前企業的營銷成本幾乎都是公開的,技術也都是透明的,促銷手段也是高度「克隆」而來的,因此僅僅依靠傳統的競爭方式(包括價格競爭)不僅難以成功,同時也對國內工程機械行業帶來較大的沖擊,所以企業營銷的成敗到底取決于什麼就成了各企業關注的焦點。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面,用於定向固定htk單克隆抗體,成功研製了檢測htk的壓電石英晶體傳感器,並基於標準scatchard繪圖法,計算出免疫反應的親和常數為1 . 85 106l / mol ,證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術,設計了的檢測htk的質量放大壓電石英晶體傳感器,該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測,應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定,實驗結果為癌癥的臨床診斷與治療提供了參考。Plants are thought to remove na + from the cytoplasm by transporting it into the vacuolar or out of the cell using na + / h + exchangers localized in the vacuolar and plasma membranes, respectively. sos1 encoding a plasma membrane na + / h + antiporter and atnhxl encoding a vacuolar na + / h + antiporter were isolated from glycophytic arabidopsis thaliana, and overexpression of atnhxl and sos1 in arabidopsis thaliana increased the salt tolerance of transgenic plants significantly
目前,擬南芥細胞內控制na ~ +外排的基因sos1及離子區隔化基因atnhx1均已克隆, sos1及atnhx1在擬南芥中的過量表達顯著提高了轉基因植株的耐鹽性,開創了降低na ~ +毒害的基因操作新途徑。Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes. but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo
目前胚胎工程技術研究和開發(如體外生產胚胎和體細胞克隆等)需要大量高質量的成熟卵母細胞,但利用屠宰動物卵巢卵母細胞經過體外成熟培養而獲取的卵母細胞質量還遠不如體內成熟卵母細胞,其原因一般認為是由於缺乏體內主卵泡階段的獲能作用。There are five - star and three - star standards zhanglongjiudian the hong hotel and the world ' s largest and well - known enterprises such as golf driving ranges
還有五星級標準的長隆酒店和三星級的香江大酒店以及世界最大的高爾夫練習場等國內外知名企業。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。The control of the deep - seated structural - magmatic process over the metallogenic system around the middle - lower yangtze river reaches is shown as : ( 1 ) the mantle uplift belt is closely related to the general geological background of the metallogenic system ; ( 2 ) the primitive magma originated from different parts of the mantle uplift belt ( mantle ridge or mantle slope ) shows differences in style of formation and composition and results in different magmatic series and metallogenic subzones, respectively ; ( 3 ) the varying depths of structural - magmatic chambers may form a " three - layered structure " ( central type, network - like and ring - like ) and this is the primary factor that controls the different magmatic series and the concerned cluster of ore deposits ; ( 4 ) the alkaline basaltic magma derived from the mantle ridge forms high - potassium and calcium alkaline magma and shoshonitic magma due to its altering intensity of afc process with the lower crustal material, which has something to do with the copper - gold and iron - sulphur metallogenic subsystems, respectively
深部構造巖漿作用對長江中下游成礦帶安徽沿江地區成礦系統的控製表現為: ( 1 )地幔隆起帶與成礦系統的總體地質背景的演變密切相關; ( 2 )源於地幔隆起帶不同部位(幔脊與幔坡)的原始巖漿,其生成方式和物質組成不盡相同,它們分別產生相應的不同巖漿巖系列和成礦亞帶; ( 3 )不同深度的構造巖漿房組成中心式網格式環帶式「三層結構」 ,是控制區內不同巖漿巖系列及有關礦床集中分佈的主要因素; ( 4 )源於地幔隆起帶脊部的堿性玄武巖漿,由於與下地殼物質發生的afc作用強度不同,形成了高鉀鈣堿性巖漿和橄欖安粗巖漿,分別與銅、金成礦亞系統和鐵、硫成礦亞系統有關。The authors hold that the joint actions of numerous factors such as the upper mantle uplifted zone ( the mantle thinned zone of lithosphere ), the upper mantle anomalous area ( relatively low - velocity area ), the uplifted zone of intracrustal high - conductivity layer, the deep fault ( shear zone of lithosphere ), the margin of the crust - upper mantle uneven masses, the basement uplifted area reflected by gravity high, the magmatic belt and tectonic intersection reflected by skipped magnetic field, control the distribution of auriferous skarn deposits and copper ( associated with gold ) skarn deposits
作者認為,上地幔隆起帶(巖石圈地幔減薄帶) 、上地幔異常區(相對低速區) 、殼內高導層隆起帶、深斷裂(巖石圈剪切帶) 、地殼上地幔不均勻性塊體的邊緣、重力高反映的基底隆起區、跳躍磁場反映的巖漿巖帶和構造交匯處等諸多因素的共同作用控制著含金夕卡巖礦床和銅伴生金夕卡巖礦床的分佈。The fractal dimensions of elevation isolines in 6 studying sections from 3 different types of geomorphological zones, including high mountain, low - moderate mountain and catchment basin, are computed by using successive coarsening method. the results show that all the studied isolines are statistical fractals, and the fractal dimensions take on different characters for different geomorphological types and scale ranges
在0 . 1 - 10km標度范圍內,等高線分維值總體表現為最新隆起區(中低山區) >強烈隆起區(高山區)沉積凹陷區(盆地區) ;在5 - 200km標度范圍內則表現出強烈隆起區最新隆起區沉積凹陷區。Inside the hall hangs a plaque inscribed and bestowed by emperor qianlong of the qing dynasty
殿內正中上方高懸清朝乾隆皇帝所賜「涌Himalayan orogen is by far the youngest and largest active continent - continent collision belt in the world and is the ideal place for studying the plate dynamics of intracontinental convergence and investigating the mechanism responsible for uplift of the plateau
喜馬拉雅造山帶是世界上目前仍在進行的、最年輕的、規模最大的陸-陸碰撞造山帶,是研究陸內匯集過程板塊動力學以及探討高原隆升機制的理想場所。The toxicity of 5 % chlorfluazuron, 4. 5 % beta - cypemethrin and the different mixtures of 5 % chlorfluazuron and 4. 5 % beta - cypemethrin to plutella xylostella larvae was determined by leaf immersion method under laboratory condition
摘要採用浸葉法在室內條件下測定5 %定蟲隆、 4 . 5 %高效氯氰菊酯單劑以及不同配比混劑對小菜蛾幼蟲的毒力。Conclusion constructed the high - level expression clone of echistatin in e. coli. the expression of recombinant protein is higher, it make the further study of echistatin feasible
結論成功構建了echistatin的原核高效表達克隆,表達量高於現有國內外研究水平,為echistatin功能及相關疾病的研究奠定了基礎。It can be futher verified by the test result that the pstvd found in the northeast region of china may be introduced into china by following the introduction of the potato cultivar irish cobbler to various countries and irish cobbler was imported in heilongjiang province prior to the 1960s
在國內,首次對中國分離株系進行cdna基因克隆和序列分析,進一步驗證了中國株系的由來及其結構特點,對于中國在pstvd檢測水平的提高、檢測試劑盒制備、 pstvd的防治及其致病機理、轉基因育種等提供重要依據。Request prices within the range ladder, language and additionally on projection, evaluation, increase as well as further on interactive can be enquired with changsha xicheng electronic co., ltd. by email
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