隆守 的英文怎麼說

中文拼音 [lōngshǒu]
隆守 英文
takamori
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 守 : Ⅰ動詞1 （防守; 看守） guard; defend 2 （守候; 看護） keep watch 3 （遵守; 遵循） observe; abide b...
  1. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。
  2. In kuala lumpur in malaysia nahmen rund 300 menschen an einer mahnwache teil

    在馬來西亞的吉隆坡，大約300人參加了守夜活動。
  3. By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells

    將得到的序列在genbank和embl數據庫中進行了同源比較，結果顯示：本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異，編碼的氨基酸有3個氨基酸殘基的差異，同源性為98 ，影響蛋白質高級結構的保守二硫鍵為2個。
  4. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  5. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  6. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  7. Honouring the contract and keeping one's word are the basis of good business.

    重合同，守信用是生意興隆的基礎。
  8. Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in

    從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明，該基因雖然有著細微的種族特異性，但高度保守並獲得了它的截斷型重組蛋白，根據蛋白一級結構和同源比較方法，模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬，預測了它們之間相互作用的特徵。
  9. We designed degenerate primers of consensus amino acid sequences of ent - kaurene oxdase, and use them to amply the middle segments of apple, strawberry and peach, and then we designed specific primers insides these middle segments to amplify 3 " termini, and 5 " termini. finally we got the full length of strawberry and 3 ' teminal of peach

    利用保守區域的兼并引物克隆了平邑甜茶，豐香草莓和碧桃貝殼杉烯氧化酶基因的長為410bp的中間片段，以中間片段序列為線索又克隆到萆莓貝殼杉烯氧化酶的全長，和碧桃此酶的3 』端。
  10. This article discusses the appearance of " the study of confucian classics " in northern song dynasty among jianlong and qingli periods emphatically and finds that ii shows a distinctive duality of conservation and innovation

    摘要北宋建隆至慶歷間的經學在謹守註疏之學的同時，也開始進行反思與突破。
  11. Our corporate brand is highly respected, with the people of hong kong clearly believing in and relying upon hongkong post. we do all we can to honour our pledges and we certainly treasure our brand

    香港郵政這個金漆招牌，備受市民信賴，而我們亦竭盡所能，恪守服務承諾，確保商譽日隆。
  12. E yang xiu, a great scholar of the song dynasty, built the pingshan pavilion when he was the mayor of the city. the royal gardens of emperor kantqxi and emperor qianlong are now well stocked with a wealth of cultural relics

    瘦西湖終點是蜀崗大明寺，唐鑒真和尚由此受邀東渡日本宋歐陽修為揚州郡守時建有平山堂康熙乾隆的御花園藏珍聚寶。
  13. At the end of the shouxi lake on shugang hill stands the daming temple where monk jianzhan of the tang dynasty started his journey eastward to japan. e yang xiu, a great scholar of the song dynasty, built the pingshan pavilion when he was the mayor of the city

    瘦西湖終點是蜀崗大明寺，唐鑒真和尚由此受邀東渡日本宋歐陽修為揚州郡守時建有平山堂康熙乾隆的御花園藏珍聚寶。
  14. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  15. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，並在序列保守區域設計簡並引物，然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 ， 17 - hsd3的中間片段。
  16. A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron

    根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。
  17. To elucidate further the presense and function of integrin - like protein, we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits

    而要進一步從分子水平上證實類整合素的存在以及研究它的功能，必須克隆其基因。根據動物整合素、亞基的胞質域保守序列設計簡並引物，利用3 race的方法擴增、亞基的3末端序列。
  18. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  19. In the part i, the sequence of the cloned promoter osg6b " was first analyzed. osg6b " had a whole length of 1688 bp and 98 % homology to known sequence of promoter osg6b. its transcriptional start point, tata boxes and the consensus sequences " tgtgg " conserved usually in anther - specific promoters were identical to those of reported osg6b

    第一部分：對已克隆的啟動子osg6b 』進行的序列分析表明， osg6b 』全長1688bp ，與報道的osg6b有98的同源性，兩者在轉錄起始位點、 tatabox及其它花藥特異性啟動子共有的保守序列tgtgg完全相同。
  20. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
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