隆法特 的英文怎麼說
中文拼音 [lōngfǎtè]
隆法特
英文
johan lonfat-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。Gene cloning provides a means of purifying and propagating specific dna segments.
基因克隆化提供了一種純化和擴增特定DNA片段的方法。The sobriquet of la carconte had been bestowed on madeleine radelle from the fact that she had been born in a village, so called, situated between salon and lambesc ; and as a custom existed among the inhabitants of that part of france where caderousse lived of styling every person by some particular and distinctive appellation, her husband had bestowed on her the name of la carconte in place of her sweet and euphonious name of madeleine, which, in all probability, his rude gutteral language would not have enabled him to pronounce
卡爾貢特娘們這個綽號的由來，是因為她出生的村莊位於薩隆和蘭比克之間，那個村莊就叫這個名字。而據卡德魯斯所住的法國那一帶地方的風俗，人們常常給每一個人一個獨特而鮮明的稱呼，她的丈夫之所以稱她卡爾貢特娘們，或許是因為瑪德蘭這三個字太溫柔，太優雅了，他那粗笨的舌頭說不慣。A man and his hund. in the film, hitler teaches his alsatian blondi to give the " sieg heil " greeting and dresses it in an ss uniform
這是一個男人和他的獵狗！影片中，希特勒教他的「阿爾撒蒂安?蒲隆地」做出德國法西斯式的「勝利」手勢，並給它穿上納粹黨衛軍的制服。However, only a few host factors with clear in vivo function have been identified. by using pcr and 5 " and 3 " race, we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene, tom1, which supports the replication of tobacco mosaic virus
根據從擬南芥中已經克隆的支持煙草花葉病毒復制的基因tom1序列設計一對特異性引物，用rt - pcr的方法獲得番茄同源基因的部分序列，然後利用5 』 race與3 』 race方法從番茄中克隆出全長ttom1基因。Kevin oeffinger of the memorial sloan - kettering cancer center in new york was lead author of the study
位於紐約的斯隆-卡特靈癌癥中心的凱文?奧法格是這項研究的主要發起者。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Has a higher application temperature than teflon ? seated flanged ball valves
比特氟隆閥座法蘭球閥還高的應用溫度。The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。It still remains a question whether the rearrangements of igh come from h / rs cell or the background lymphocytes. in this study, we have detected the igh clonal correlation between the h / rs cells and the background cells, from a new aspect to study the clonality of h / rs cell and its relation with the background cells. the expression of b - cell - specific activator protein ( bsap ) was detected in hl. igh gene rearrangements were analysed by the methods including gene analysis in neoplasms tissue and micropicked cells from paraffin - embedded sections, sequencing to test the pcr product, and in situ pcr
本研究將在以往研究的基礎上，在國內率先把b細胞核反式作用因子? b細胞特異性激活蛋白（ b - cell - specificactivatorprotein ， bsap ）應用於hl的研究，檢測hl的bsap表達，並採用石蠟刮片組織和微切割單細胞的基因分析、測序分析和間接原位pcr等方法，同步觀察分析h rs和背景淋巴細胞的igh基因克隆相關性，從又一個新視角探究chl的腫瘤性h rs細胞克隆性及與背景淋巴細胞的關系。2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。The company did not immediately return a call from the associated press wednesday. the suit alleges that cornett bought the peanuts at an o ' fallon store last august and later was eating them at his home when he bit into a " non - human animal tooth.
起訴書中稱，去年8月份，科內特在美國奧法隆市的一家商店裡購買了一包花生，他回到家吃花生時從中發現了一顆「非人類的動物牙齒」 。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1Jacques doillon, the french director of " " ponette " ", seems to have a special radar into the inner lives of children
雅克?多爾隆是執導《波妮特》的法國導演，看來對小孩子的生活有特別的洞察力。Scientists like ian wilmut at the roslin research institute near edinburgh, the man who cloned dolly the sheep, has warned that british expertise will go to america, where such work is legal, if the government does not give the go - ahead14
一些科學家，如在愛丁堡附近的羅斯林研究所里曾克隆多莉羊的伊恩?維爾姆特，警告說如果英國政府不批準繼續克隆研究，英國的這項專業技術就會流向美國，因為在美國克隆技術是合法的。With applying dsst algorithm, mobile agent can solve the problem of selecting router. synthesizing advantages of centralized search engine and distributed search engine, through it that system obtains the best way that agents mobile does. after statistics analysis, the author gets the mathematic pattern that mobil agent clones right times
利用idl語言定義了agent在多種平臺上移動的介面，設計了一個符合maf規范的插件，實現agent在異種平臺之間的移動；利用動態最小生成樹演算法解決移動agent的路由選擇問題，綜合集中式搜索和分散式搜索的特點，動態尋找出一條agent移動的最佳路線；通過統計分析得到移動agent克隆的數學模型，通過計算可以獲得agent克隆個數的最佳值。Distributing pattern of soil properties, including soil moisture and nutrients beneath the natural shrubs of sabina vulgaris and its ecological adaptability of clonal traits to sandy habitats in mu us sandland were studied using field experiments
應用野外實驗生態學方法研究了毛烏素沙地天然臭柏灌叢地土壤資源的分佈格局及天然臭柏克隆特性對沙地環境的生態適應性。It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his
本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. to clone and identify new genes that control the heart development, by a way of chemical mutagen, ems, we have established 1, 200 balanced - lethal lines on chromosome 2 and 3. with the screening the 330 stocks with immunochemical method using heart - specific antibody, mab. no. 3, we detected 60 lethal lines showing heart mutant phynotype
為了克隆和鑒定控制心臟發育的新基因，本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅，並且建立了1200個第二和第三染色體的平衡致死系，利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選，觀察到有60個致死系表現出心臟突變表型， 20個品系的心臟突變表型有待進一步證實。分享友人