隨機引物標記 的英文怎麼說

中文拼音 [suíjīyǐnwùbiāojì]
隨機引物標記 英文
random primer labelling
  • 隨 : Ⅰ動詞1 （跟; 跟隨） follow 2 （順從） comply with; adapt to 3 （任憑; 由著） let （sb do as he li...
  • 機 : machineengine
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
  • 記 : Ⅰ動詞1 （把印象保持在腦子里） remember; bear in mind; commit to memory 2 （記錄; 記載;登記） writ...
  • 隨機 : random stochasticrandom
  • 標記 : （標志; 記號; 貨物標記） tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
  1. Two hundred and seventy - four sites were detected by 15 10 - mer primers, out of which 262 were polymorphic ( 95. 6 % of the total loci detected ). all of the materials were clearly distinguished, of which fourteen samples have forty - five typical rapd bands. before the molecular taxonomy was introduced, people never doubted subgenus yulania ' s disrelation with subgenus magnolia and a close relation between subgenus yulania and michelia, as there is in both genera a sameness seen in their prolepsis and powder chambers dehiscing on the side

    3 、通過對玉蘭亞屬rapd擴增譜帶的分析，發現該類群具有很高的多態性：多態位點百分率高達95 . 6 ，產生的多態性帶數與所用引物數的比例為17 . 6 ， 15條隨機引物清楚地分開了31個種類，其中的14個種共有45條特徵性帶，在利用rapd標記已研究過的類群中，本類群多態性較高。
  2. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  3. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  4. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  5. The double chains of chil - 1 cdna were labeled with dig - 11 - dutp to produce probe. and the chil - 1 mrna secreation was studied in the tissues of chickens " brains infected with aev and that of normal chickens ". the results showed that the content of chil - 1 secreation in sick chickens " brain was higher than that of the normal chickens "

    用地高辛隨機引物法標記il - 1 cdna制備探針，原位雜交檢測禽腦脊髓炎病雞與正常雞腦組織中il - 1分泌情況，結果表明腦炎病雞腦中il - 1分泌量高於正常雞。
  6. The main difficulty in development of microsatellite ( or simple sequence repeats, ssr ) is to screen the specific sequences flanking ssr, a method to screen microsatellite based on sampl was tested in the chinese shrimp. randomly selective bands ( s1k s13 > s14 > s22 > s24 ) were cloned and sequenced, 61bp, i57bp 147bp, 152bp, 298bp sequences were obtained respectively. no ssr were found inslk s s s22 apart from the sampl primers sequence, on the other hand, a ssr sequence of ( ga ) 39 were found in s24, which comprises aflp primers only

    微衛星的發展主要受限於微衛星兩端旁側特異引物的篩選，本研究在中國對蝦中初步嘗試了基於sampl技術的微衛星標記開發，隨機選取了5個s枷pl條帶（ 511 、 513 、 514 、 522 、 524 ）進行了克隆測序，大小分別為161bp 、 157bp 、 147bp 、 152bp 、 298bp ，但在511 、 513 、 514 、 522四個片段中未找到引物序列外的微衛星序列重復; 524兩端引物均為傳統的aflp引物，在此測序片段中有一（ ga ） 39重復序列。
  7. The analysis of genetic diversity of 10 families in p. taiwanensis hayata. was carried out by rapd molecular markers, which may provide the theoretical base for breeding. 17 primers were screened from 206 random oligonucleotide primers and 38 polymorphic loci were obtained from pcr of 17 primers

    利用rapd分子標記分析了黃山松10個家系的遺傳多樣性，為育種提供依據，從206個隨機引物中篩選出17個引物，獲得38個多態位點。
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