This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和
牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的
雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
Beecheyana is one of the gynodiecious ficus spp ; the female syconium and male syconiu are simular outside syconium, but obviously different inside the syconium which showed the dimorphoism
牛奶榕為
雌雄異株的榕屬植物,
雌雄榕果在榕果外部形態相似,在榕果內部有明顯差異,呈現兩型性。
As said before he ate with relish the inner organs, nutty gizzards, fried cods roes while richie goulding, collis, ward ate steak and kidney, steak then kidney, bite by bite of pie he ate bloom ate they ate
正如前文118所說的,他吃起下水有嚼頭的胗和炸
雌鱈卵來真是津津有味。考立斯-沃德律師事務所的里奇古爾丁則吃著
牛排配腰子餅。他先吃
牛排,然後吃腰子。
In order to improve the cuture system of mammal embryo and advance the quality of embryo in vitro, the effects of taurine and ethylenediaminetetraacetic acid ( edta ), estrogen ( e2 ) and progesterone ( p ) on preimpiantation development of mouse embryos in vitro were examined and the quality difference of blastocysts from in vitro and in vivo by blastocyst staining and embryo transfer was evaluated
為完善體外培養體系,提高體外發育的胚胎質量,本實驗研究了
牛磺酸( tau ) 、 edta 、
雌激素( e2 )和孕激素( p )對昆明白小鼠胚胎體外發育的影響。在此基礎上,通過胚胎移植技術觀察了不同來源的囊胚在移入子宮后的發育情況,以比較體外培養和體內發育的囊胚質量。
For the first olympian sacrifice, the taboos surrounding the sacred kine of apollo had to be transgressed, and the trickster god of boundaries was the one to do it
在首次奧林匹克運動會的獻祭上,圍繞在阿波羅的神聖
雌牛周圍的禁忌已經被違反了,魔術師般的邊界之神是其中一位始作俑者。
Effect of activation way on the parthenogenetic development of buffalo oocytes
激活方法對水
牛卵母細胞孤
雌發育的影響
Effects of 6 - dimethylaminopurine on the parthenogenetic development of bovine oocytes
二甲氨基嘌呤對
牛卵母細胞孤
雌發育的影響
The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用
牛精子免疫
雌性四周齡balb c小鼠,從其脾臟組織分離總rna ,應用重組噬菌體抗體庫技術,構建了一個針對
牛精子的噬菌體抗體文庫。
Compared with the cytoplasts matured in the maturation medium directly for 16 ~ 18h, the fusion rate, cleavage rate and blastocyst development of reconstructed embryos from oocytes cultured in 2mmol / l 6 - dmap for 3 - 5h prior to ivm did not differ significantly from that of reconstructed embryos from oocytes matured in the maturation medium directly for 16 ~ 18h
水
牛卵母細胞先經水
牛卵母細胞孤
雌激活與體細胞核移植的研究6 dmap抑製成熟培養3 sh ,再在正常成熟培養液中成熟培養16 18h 。結果發現,與直接在正常成熟培養液中成熟培養16 18h的卵母細胞相比,其融合率,分裂率和囊胚發育率均無顯著影響。
She is a 5 - year old female asian water buffalo and wwf introduced her into a 1. 4 hectare freshwater marshland home, giving her 18 - months to show how she can help to increase wildlife diversity inside the reserve, such as attracting cattle egrets, snipe and dragonflies
今年五月,世界自然基金會展開一項嶄新的研究項目,並由一頭5歲的
雌性亞洲水
牛負責,往後18個月它將以米埔保護區內一塊面積1 . 4公頃的淡水沼澤地為家。
Parthenogenetic activation of buffalo oocytes and compare embryo development after chemical activation and in vitro fertilized
水
牛卵母細胞孤
雌激活及孤
雌胚與體外受精胚發育比較
Parthenogenetic activation of buffalo oocytes and compare embryo development after chemical activation and in vitro fertilization
水牛卵母細胞孤雌激活及孤雌胚與體外受精胚發育的比較